ECMDB: Probable acetyl-CoA acetyltransferase (Q46939)

Identification

Name:

Probable acetyl-CoA acetyltransferase

Synonyms:

Acetoacetyl-CoA thiolase

Gene Name:

yqeF

Enzyme Class:

2.3.1.9

Biological Properties

General Function:

Involved in transferase activity, transferring acyl groups other than amino-acyl groups

Specific Function:

2 acetyl-CoA = CoA + acetoacetyl-CoA

Cellular Location:

Cytoplasm (Potential)

SMPDB Pathways:

Fatty acid metabolism PW000796
Propanoate metabolism PW000940
Tryptophan metabolism PW000815

KEGG Pathways:

Benzoate degradation via CoA ligation ec00632
Benzoate degradation via hydroxylation ec00362
Butanoate metabolism ec00650
Fatty acid metabolism ec00071
Glyoxylate and dicarboxylate metabolism ec00630
Lysine degradation ec00310
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Propanoate metabolism ec00640
Pyruvate metabolism ec00620
Reductive carboxylate cycle (CO2 fixation) ec00720
Synthesis and degradation of ketone bodies ec00072
Terpenoid backbone biosynthesis ec00900
Tryptophan metabolism ec00380
Valine, leucine and isoleucine degradation ec00280

KEGG Reactions:

2.0

↔

1.0

2.0Acetyl-CoA ↔ 1.0Acetoacetyl-CoA + 1.0Coenzyme A
ReactionCard

1.0

1.0Butanoyl-CoA

↔

1.0

1.0Acetyl-CoA + 1.0Butanoyl-CoA ↔ 1.0Coenzyme A + 1.03-Oxohexanoyl-CoA
ReactionCard

EcoCyc Reactions:

2.0

↔

1.0

2.0Acetyl-CoA ↔ 1.0Acetoacetyl-CoA + 1.0Coenzyme A
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB03943	3-Oxohexanoyl-CoA	MetaboCard
ECMDB01484	Acetoacetyl-CoA	MetaboCard
ECMDB01206	Acetyl-CoA	MetaboCard
ECMDB01088	Butyryl-CoA	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard

GO Classification:

Function
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
metabolic process

Gene Properties

Blattner:

b2844

Gene Orientation

Counterclockwise

Centisome Percentage:

64.28

Left Sequence End

2982433

Right Sequence End

2983614

Gene Sequence:

>1182 bp
ATGACCTGTTCAACTTCATTAAGCGGCAAAAACAGGATTGTCCTTATCGCTGGCATTCTG
ATGATTGCCACAACATTACGCGTCACCTTTACCGGCGCAGCACCGTTACTGGATACGATT
CGTTCCGCTTACTCGCTGACGACAGCGCAAACCGGCTTATTGACCACCCTGCCATTATTG
GCCTTTGCGCTAATCTCACCTTTGGCTGCCCCGGTAGCGCGACGTTTTGGTATGGAACGT
AGCCTGTTTGCCGCGTTACTTTTGATCTGTGCTGGTATCGCAATTCGCTCTCTCCCTTCG
CCTTACTTATTATTTGGCGGTACAGCGGTCATTGGCGGTGGGATTGCATTAGGCAATGTC
TTACTGCCAGGATTAATTAAACGCGATTTCCCTCATTCCGTCGCCAGACTTACCGGCGCA
TATTCCCTGACAATGGGAGCTGCAGCGGCACTGGGATCGGCTATGGTCGTGCCGCTGGCT
TTGAACGGTTTTGGCTGGCAAGGCGCGTTGCTCATGCTGATGTGTTTTCCTCTGCTGGCT
CTTTTTTTATGGCTGCCACAGTGGCGAAGTCAACAACATGCAAATTTGAGTACCTCGCGC
GCCTTACATACTCGGGGTATCTGGCGTTCACCGCTTGCCTGGCAGGTCACATTGTTTCTT
GGGATCAACTCACTGGTCTATTACGTGATTATTGGCTGGCTTCCGGCGATCCTCATCAGT
CACGGCTATAGCGAAGCACAGGCGGGTTCACTGCATGGTTTGCTGCAACTAGCCACAGCA
GCACCCGGTTTGCTGATCCCACTTTTCTTACATCATGTGAAAGATCAGCGTGGTATTGCA
GCGTTCGTTGCCTTGATGTGCGCAGTGGGCGCGGTTGGGCTCTGCTTTATGCCAGCGCAC
GCGATCACCTGGACTCTGCTTTTCGGTTTTGGTTCCGGCGCAACAATGATACTGGGGTTG
ACGTTCATTGGTCTGCGGGCTAGTTCTGCGCATCAGGCGGCGGCACTCTCGGGGATGGCA
CAATCCGTCGGGTATTTGTTGGCAGCCTGTGGGCCGCCGCTGATGGGTAAAATACACGAT
GCTAACGGTAACTGGTCTGTACCACTTATGGGTGTTGCCATACTTTCACTACTGATGGCG
ATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAA

Protein Properties

Pfam Domain Function:

Thiolase_C (PF02803 )
Thiolase_N (PF00108 )

Protein Residues:

393

Protein Molecular Weight:

41018

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Probable acetyl-CoA acetyltransferase
MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVPAYAVDEVILGQVLTAG
AGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAIQCGEADIVIAGGQENMSR
APHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVTAENLAREYGISRQLQDAYAL
SSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQPRTDASAEGLARLNPSFDSLGS
VTAGNASSINDGAAAVMMMSEAKARALNLPVLARIRAFASVGVDPALMGIAPVYATRRCL
ERVGWQLAEVDLIEANEAFAAQALSVGKMLEWDERRVNVNGGAIALGHPIGASGCRILVS
LVHEMVKRNARKGLATLCIGGGQGVALTIERDE

References

External Links:

Resource	Link
Uniprot ID:	Q46939
Uniprot Name:	YQEF_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1736416
Ecogene ID:	EG13097
Ecocyc:	EG13097
ColiBase:	b2844
Kegg Gene:	b2844
EchoBASE ID:	EB2900
CCDB:	YQEF_ECOLI
BacMap:	90111494

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553