Cysteine sulfinate desulfinase (Q46925)

Identification
Name:Cysteine sulfinate desulfinase
Synonyms:
  • CSD
Gene Name:csdA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in metabolic process
Specific Function:Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine. L-cysteine sulfinic acid is the best substrate. Functions as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0Thumb+2.0Thumb → 1.0Thumb+1.0Thumb
1.03-Sulfinoalanine + 2.0Hydrogen ion → 1.0L-Alanine + 1.0Sulfur dioxide
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB009963-SulfinoalanineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00161L-AlanineMetaboCard
ECMDB21359Sulfur dioxideMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
cysteine desulfurase activity
pyridoxal phosphate binding
sulfurtransferase activity
transferase activity
transferase activity, transferring sulfur-containing groups
Process
biosynthetic process
cellular biosynthetic process
cofactor biosynthetic process
iron-sulfur cluster assembly
metabolic process
metallo-sulfur cluster assembly
Gene Properties
Blattner:b2810
Gene OrientationClockwise
Centisome Percentage:63.40
Left Sequence End2941359
Right Sequence End2942564
Gene Sequence:
>1206 bp
ATGCGTGAAGCCTTTATTTGTGACGGAATTCGTACGCCAATTGGTCGCTACGGCGGGGCA
TTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGA
AACCCGCGTCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCG
GGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGT
GTTTCCGGCACAACCATTAACCGCTTGTGTGGTTCCGGGCTGGACGCACTGGGGTTTGCC
GCACGGGCGATTAAAGCGGGCGATGGCGATTTGCTGATCGCCGGTGGCGTGGAGTCAATG
TCACGGGCACCGTTTGTTATGGGCAAGGCAGCCAGTGCATTTTCTCGTCAGGCTGAGATG
TTCGATACCACTATTGGCTGGCGATTTGTGAACCCGCTCATGGCTCAGCAATTTGGAACT
GACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGAT
CAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATT
CTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATA
CAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCA
CCATTTCGTGCCAATGGGGTGATTACCGCAGGCAATGCTTCCGGGGTGAATGACGGAGCC
GCTGCGTTGATTATTGCCAGTGAACAGATGGCAGCAGCGCAAGGACTGACACCGCGGGCG
CGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTG
CCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATT
GAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCT
GATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGA
ATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGT
TACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGT
GTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:401
Protein Molecular Weight:43234
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Cysteine sulfinate desulfinase
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYSLSAGNVHRSQFAEAQR
LTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHH
ANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPRSRILALGQMSNVTGGCPDLA
RAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKSELLE
AMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESW
SRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQ
PLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLVD
References
External Links:
ResourceLink
Uniprot ID:Q46925
Uniprot Name:CSDA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742272
Ecogene ID:EG13082
Ecocyc:EG13082
ColiBase:b2810
Kegg Gene:b2810
EchoBASE ID:EB2891
CCDB:CSDA_ECOLI
BacMap:16130717
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lacourciere, G. M., Mihara, H., Kurihara, T., Esaki, N., Stadtman, T. C. (2000). "Escherichia coli NifS-like proteins provide selenium in the pathway for the biosynthesis of selenophosphate." J Biol Chem 275:23769-23773. Pubmed: 10829016
  • Mihara, H., Kurihara, T., Yoshimura, T., Esaki, N. (2000). "Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions." J Biochem 127:559-567. Pubmed: 10739946
  • Mihara, H., Kurihara, T., Yoshimura, T., Soda, K., Esaki, N. (1997). "Cysteine sulfinate desulfinase, a NIFS-like protein of Escherichia coli with selenocysteine lyase and cysteine desulfurase activities. Gene cloning, purification, and characterization of a novel pyridoxal enzyme." J Biol Chem 272:22417-22424. Pubmed: 9278392