7-cyano-7-deazaguanine synthase (P77756)

Identification
Name:7-cyano-7-deazaguanine synthase
Synonyms:
  • 7-cyano-7-carbaguanine synthase
  • PreQ(0) synthase
  • Queuosine biosynthesis protein queC
Gene Name:queC
Enzyme Class:
Biological Properties
General Function:Involved in argininosuccinate synthase activity
Specific Function:Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.07-Deaza-7-carboxyguanine + 1.0Ammonia + 1.0Adenosine triphosphate ↔ 1.07-Cyano-7-carbaguanine + 1.0ADP + 1.0Phosphate + 1.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.07-Deaza-7-carboxyguanine + 1.0Adenosine triphosphate + 1.0Ammonium → 1.0Water + 1.0Phosphate + 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.07-Cyano-7-carbaguanine + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.07-carboxy-7-deazaguanine+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.07-carboxy-7-deazaguanine + 1.0Ammonia + 1.0Adenosine triphosphate → 1.07-Cyano-7-carbaguanine + 1.0ADP + 1.0Phosphate + 1.0Water
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.07-Deaza-7-carboxyguanine + 1.0Ammonium → 1.0ADP + 1.0Hydrogen ion + 1.0Water + 1.0Phosphate + 1.07-Cyano-7-carbaguanine
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.07-Deaza-7-carboxyguanine + 1.0Ammonia + 1.0Adenosine triphosphate → 1.07-Cyano-7-carbaguanine + 1.0ADP + 1.0Inorganic phosphate + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB202947-Cyano-7-carbaguanineMetaboCard
ECMDB211837-Deaza-7-carboxyguanineMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00051AmmoniaMetaboCard
ECMDB21186AmmoniumMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
argininosuccinate synthase activity
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
nucleoside binding
purine nucleoside binding
Process
arginine biosynthetic process
arginine metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
metabolic process
Gene Properties
Blattner:b0444
Gene OrientationCounterclockwise
Centisome Percentage:10.00
Left Sequence End464076
Right Sequence End464771
Gene Sequence:
>696 bp
ATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGA
CTTGAACCGCAAGCAGAAGATGCGCCGCTTAACAACTATGCTAACGAGCGGCTTACCCAA
CTTAACCAACAGCAGCGCTGTGACGCGTTTATTCAGTTTGCCAGCGATGTTATGTTGACG
CGCTGTGAGCTGACCAGCGAGGCGAAGGCGGCAGAAGCTGCAATACGTCTGTGTAAAGAG
CTGGGAGATCAGTCGGTCGTGATTAGCGGTGACACGAGGCTGGAGGAATTGGGGATTAGC
GAACGTTTGCAGCAGGAATGCAATGCCGTTGTTTGGGATCCGGCGAAAGGTGCCGAGAAT
ATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAA
TCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCG
GAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCA
GAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATT
AGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGGCCCG
GTGAAAGCGGTGTATCTGATTATTGAGGATTGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:231
Protein Molecular Weight:25514
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>7-cyano-7-deazaguanine synthase
MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV
LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI
TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL
VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQKTGLR
References
External Links:
ResourceLink
Uniprot ID:P77756
Uniprot Name:QUEC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674452
Ecogene ID:EG13252
Ecocyc:EG13252
ColiBase:b0444
Kegg Gene:b0444
EchoBASE ID:EB3041
CCDB:QUEC_ECOLI
BacMap:16128429
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gaur, R., Varshney, U. (2005). "Genetic analysis identifies a function for the queC (ybaX) gene product at an initial step in the queuosine biosynthetic pathway in Escherichia coli." J Bacteriol 187:6893-6901. Pubmed: 16199558
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553