3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component (P77650)

Identification
Name:3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component
Synonyms:
  • Digoxigenin system ferredoxin--NAD(+) reductase component
Gene Name:hcaD
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Part of the multicomponent 3-phenylpropionate dioxygenase, that converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP- dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0NADH + 1.0Oxygen → 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard
2.0Reduced rubredoxin+1.0Thumb ↔ 2.0Oxidized rubredoxin+1.0Thumb+1.0Thumb
2.0Reduced rubredoxin + 1.0NAD ↔ 2.0Oxidized rubredoxin + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.04-Chlorobiphenyl+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxy-4'-chlorobiphenyl+1.0Thumb
1.04-Chlorobiphenyl + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxy-4'-chlorobiphenyl + 1.0NAD
ReactionCard
1.04-Chlorobiphenyl+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxy-4'-chlorobiphenyl+1.0Thumb
1.04-Chlorobiphenyl + 1.0Oxygen + 1.0NADPH + 1.0Hydrogen ion ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxy-4'-chlorobiphenyl + 1.0NADP
ReactionCard
1.0Biphenyl+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxybiphenyl+1.0Thumb
1.0Biphenyl + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxybiphenyl + 1.0NAD
ReactionCard
1.0Biphenyl+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxybiphenyl+1.0Thumb
1.0Biphenyl + 1.0Oxygen + 1.0NADPH + 1.0Hydrogen ion ↔ 1.0cis-2,3-Dihydro-2,3-dihydroxybiphenyl + 1.0NADP
ReactionCard
1.0Ethylbenzene+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0cis-1,2-Dihydro-3-ethylcatechol+1.0Thumb
1.0Ethylbenzene + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-1,2-Dihydro-3-ethylcatechol + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrocinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0trans-Cinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrocinnamic acid + 1.0NADH + 1.0Oxygen → 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0NAD + 1.0Hydrogen ion ↔ 1.0NADH
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0Oxygen + 1.0NADH → 1.0Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0trans-Cinnamic acid + 1.0Hydrogen ion + 1.0NADH + 1.0Oxygen → 1.0cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrocinnamic acid + 1.0Oxygen + 1.0NADH + 1.0Hydrogen ion ↔ 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrocinnamic acid + 1.0NADH + 1.0Oxygen → 1.0cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diol + 1.0NAD
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0NADH + 1.0Oxygen + 1.0Hydrocinnamic acid → 1.0Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diol + 1.0NAD
ReactionCard
1.0Reduced ferredoxin+1.0Thumb → 1.0oxidized ferredoxin+1.0Thumb
1.0Reduced ferredoxin + 1.0NAD → 1.0oxidized ferredoxin + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20129cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diolMetaboCard
ECMDB21320Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diolMetaboCard
ECMDB20130cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diolMetaboCard
ECMDB00764Hydrocinnamic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB00930trans-Cinnamic acidMetaboCard
GO Classification:
Function
adenyl nucleotide binding
binding
catalytic activity
FAD or FADH2 binding
nucleoside binding
oxidoreductase activity
purine nucleoside binding
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2542
Gene OrientationClockwise
Centisome Percentage:57.55
Left Sequence End2670069
Right Sequence End2671271
Gene Sequence:
>1203 bp
ATGACGCAATACTCCTCTCTCCTTCGTGGTCTTGCAGCGGGTTCTGCATTTTTATTCCTT
TTTGCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGT
GCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAG
AAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTT
AAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACG
GGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCG
GTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTG
GCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATGGTTATGCCAAA
AAACTGGGTCTGACCAACACTACCTTCCAGACGGTGCACGGCCTGGATGCGCCGGGGCAG
TTCAGCACCGCGCGCGATATGGCATTGCTGGGTAAAGCATTGATCCACGATGTGCCGGAA
GAGTACGCCATTCATAAAGAGAAAGAGTTCACCTTCAACAAAATTCGTCAGCCTAACCGT
AACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCA
GGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTA
GTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGG
GGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAG
CGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTG
ACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATACGTTAACGGAACCGCAG
CTTACCGCACCGCTGAAAAAAGGTCAGGTTGTCGGGACCATTGATTTCCAGCTTAACGGT
AAATCCATTGAGCAGCGTCCGCTGATCGTGATGGAAAATGTGGAAGAGGGCGGATTCTTT
GGTCGGGTGTGGGATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCT
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:400
Protein Molecular Weight:43978
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD(+) reductase component
MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYERPPLSKSMLLEDSPQLQ
QVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWDQLFIATGAAARPLPLLDA
LGERCFTLRHAGDAARLREVLQPERSVVIIGAGTIGLELAASATQRRCKVTVIELAATVM
GRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKVELTLQSGETLQADVVIYGIGIS
ANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDVAITRLDNGALHRCESWENANNQAQ
IAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDMRGDDWLCRGNPETQKAIWFNLQNGVL
IGAVTLNQGREIRPIRKWIQSGKTFDAKLLIDENIALKSL
References
External Links:
ResourceLink
Uniprot ID:P77650
Uniprot Name:HCAD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651381
Ecogene ID:EG13460
Ecocyc:EG13460
ColiBase:b2542
Kegg Gene:b2542
EchoBASE ID:EB3233
CCDB:HCAD_ECOLI
BacMap:16130467
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Diaz, E., Ferrandez, A., Garcia, J. L. (1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." J Bacteriol 180:2915-2923. Pubmed: 9603882
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837