ECMDB

Identification

Name:

Probable enoyl-CoA hydratase paaG

Synonyms:

Not Available

Gene Name:

paaG

Enzyme Class:

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Could possibly oxidize fatty acids using specific components

Cellular Location:

Not Available

SMPDB Pathways:

Fatty acid metabolism PW000796
Phenylalanine metabolism PW000921
Phenylethylamine metabolism PW002027
Propanoate metabolism PW000940
Tryptophan metabolism PW000815
beta-Alanine metabolism PW000896

KEGG Pathways:

1,4-Dichlorobenzene degradation ec00627
Benzoate degradation via CoA ligation ec00632
Benzoate degradation via hydroxylation ec00362
Biosynthesis of unsaturated fatty acids ec01040
Butanoate metabolism ec00650
Caprolactam degradation ec00930
Fatty acid elongation in mitochondria ec00062
Fatty acid metabolism ec00071
Geraniol degradation ec00281
Limonene and pinene degradation ec00903
Lysine degradation ec00310
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Phenylalanine metabolism ec00360
Propanoate metabolism ec00640
Reductive carboxylate cycle (CO2 fixation) ec00720
Tryptophan metabolism ec00380
Valine, leucine and isoleucine degradation ec00280
alpha-Linolenic acid metabolism ec00592
beta-Alanine metabolism ec00410

KEGG Reactions:

1.0

↔

1.0

1.03-Oxo-5,6-dehydrosuberyl-CoA + 1.0Coenzyme A ↔ 1.05-Carboxy-2-pentenoyl-CoA + 1.0Acetyl-CoA
ReactionCard

1.02-(1,2-Epoxy-1,2-dihydrophenyl)acetyl-CoA

1.0

↔

1.0

1.02-(1,2-Epoxy-1,2-dihydrophenyl)acetyl-CoA + 1.02-(1,2-Epoxy-1,2-dihydrophenyl)acetyl-CoA ↔ 1.02-Oxepin-2(3H)-ylideneacetyl-CoA
ReactionCard

1.0(3S)-3-Hydroxyacyl-CoA

↔

1.0

1.0trans-3-Enoyl-CoA

1.0

1.0(3S)-3-Hydroxyacyl-CoA ↔ 1.0trans-2,3-Dehydroacyl-CoA + 1.0trans-3-Enoyl-CoA + 1.0Water
ReactionCard

SMPDB Reactions:

1.02,3-didehydroadipyl-CoA

1.0

→

1.0

1.02,3-didehydroadipyl-CoA + 1.0Water → 1.03-Hydroxyadipyl-CoA
ReactionCard

EcoCyc Reactions:

1.02-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA

↔

1.0

1.02-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA ↔ 1.02-Oxepin-2(3H)-ylideneacetyl-CoA
ReactionCard

Complex Reactions:

1.0

→

1.0

1.02-(1,2-Epoxy-1,2-dihydrophenyl)acetyl-CoA → 1.02-Oxepin-2(3H)-ylideneacetyl-CoA
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB23159	2-(1,2-Epoxy-1,2-dihydrophenyl)acetyl-CoA	MetaboCard
ECMDB21164	2-Oxepin-2(3H)-ylideneacetyl-CoA	MetaboCard
ECMDB20231	3-Hydroxyadipyl-CoA	MetaboCard
ECMDB21172	3-Oxo-5,6-dehydrosuberyl-CoA	MetaboCard
ECMDB20101	5-Carboxy-2-pentenoyl-CoA	MetaboCard
ECMDB01206	Acetyl-CoA	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
Process
metabolic process

Gene Properties

Blattner:

b1394

Gene Orientation

Clockwise

Centisome Percentage:

31.39

Left Sequence End

1456288

Right Sequence End

1457076

Gene Sequence:

>789 bp
GTGATTGATAAATCCGCCTTTGTGCATCCAACCGCCATTGTGGAAGAGGGCGCGTCAATT
GGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAG
GGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAAT
GAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAA
CCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGT
GGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAAC
GCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACG
CTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCAT
CAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGAC
GTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAA
GGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAG
CTGATTTATCGTAGCGGTAAAACGCTCGATGAAGTGAAACCGGAAATTGCTGAACTGGCG
GAAACATATCCGGAAGTGAAAGCCTTTACCGATTTCTTTGCACGCTCAACGCGCGGTCTG
ATTCGTTAA

Protein Properties

Pfam Domain Function:

ECH (PF00378 )

Protein Residues:

262

Protein Molecular Weight:

28404

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Probable enoyl-CoA hydratase paaG
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVERDDTIRCLLLTGAGRGF
CAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALG
GDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARAMGLALLGNQLSAEQAHEWGM
IWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDTQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK

References

External Links:

Resource	Link
Uniprot ID:	P77467
Uniprot Name:	PAAG_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674370
Ecogene ID:	EG13741
Ecocyc:	EG13741
ColiBase:	b1394
Kegg Gene:	b1394
EchoBASE ID:	EB3504
CCDB:	PAAG_ECOLI
BacMap:	16129355

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Ferrandez, A., Garcia, J. L., Diaz, E. (2000). "Transcriptional regulation of the divergent paa catabolic operons for phenylacetic acid degradation in Escherichia coli." J Biol Chem 275:12214-12222. Pubmed: 10766858
Ferrandez, A., Minambres, B., Garcia, B., Olivera, E. R., Luengo, J. M., Garcia, J. L., Diaz, E. (1998). "Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway." J Biol Chem 273:25974-25986. Pubmed: 9748275
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553

Probable enoyl-CoA hydratase paaG (P77467)