Putative glutamate--cysteine ligase 2 (P77213)

Identification
Name:Putative glutamate--cysteine ligase 2
Synonyms:Not Available
Gene Name:ybdK
Enzyme Class:
Biological Properties
General Function:cellular modified amino acid biosynthetic process
Specific Function:ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity. However, because of the low catalytic rate, the question remains whether L-cysteine is the actual biological substrate.
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Cysteine + 1.0L-Glutamate ↔ 1.0ADP + 1.0gamma-Glutamylcysteine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0L-Cysteine + 1.0L-Glutamate ↔ 1.0ADP + 1.0gamma-Glutamylcysteine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Function
ATP binding
cellular modified amino acid biosynthetic process
glutamate-cysteine ligase activity
ligase activity, forming carbon-nitrogen bonds
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1119
atgccattacccgattttcatgtttctgaaccttttaccctcggtattgaactggaaatg
caggtggttaatccgccgggctatgacttaagccaggactcttcaatgctgattgacgcg
gttaaaaataagatcacggccggagaggtaaagcacgatatcaccgaaagtatgctggag
ctggcgacggatgtttgccgtgatatcaaccaggctgccgggcagttttcagcgatgcag
aaagtcgtattgcaggcagccacagaccatcatctggaaatttgcggcggtggcacgcac
ccgtttcagaaatggcagcgtcaggaggtatgcgataacgaacgctatcaacgcacgctg
gaaaactttggttatctcattcagcaggcgaccgtttttggtcagcatgtccatgttggc
tgcgccagtggcgatgacgccatttatttgctgcacggcttgtcacgatttgtgccgcac
tttatcgccctttccgccgcgtcgccatatatgcagggaacggatacgcgttttgcctcc
tcacgaccgaatattttttccgcctttcctgataatggcccgatgccgtgggtcagtaac
tggcaacaatttgaagccctgtttcgctgtctgagttacaccacgatgatcgacagcatt
aaagatctgcactgggatattcgccccagtcctcattttggcacggtggaggttcgggtg
atggataccccgttaacccttagccacgcagtaaatatggcgggattaattcaggctacc
gcccactggttactgacggaacgcccgtttaaacatcaggaaaaagattacctgctgtat
aaattcaaccgtttccaggcctgtcgctatgggcttgaaggcgtcatcaccgatccgcac
actggagatcgtcgaccgctaacggaagataccttgcgattgctggaaaaaatcgcccct
tccgcacataaaattggtgcatcgagcgcgattgaggccctgcatcgccaggtcgtcagc
ggtctgaatgaagcgcagctaatgcgcgatttcgtcgccgatggcggctcgctgattggg
ctggtgaaaaagcattgtgagatctgggccggtgactaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:372
Protein Molecular Weight:41688
Protein Theoretical pI:Not Available
PDB File:1R8G
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>Putative glutamate--cysteine ligase 2
MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNKITAGEVKHDITESMLE
LATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPFQKWQRQEVCDNERYQRTL
ENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPHFIALSAASPYMQGTDTRFAS
SRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMIDSIKDLHWDIRPSPHFGTVEVRV
MDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKDYLLYKFNRFQACRYGLEGVITDPH
TGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEALHRQVVSGLNEAQLMRDFVADGGSLIG
LVKKHCEIWAGD
References
External Links:
ResourceLink
Uniprot ID:P77213
Uniprot Name:GCS2_ECOLI
PDB ID:1R8G
CCDB:GCS2_ECOLI
General Reference:Not Available