ECMDB: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (P76422)

Identification

Name:

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Synonyms:

Hydroxymethylpyrimidine kinase
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
HMP-P kinase
HMP-phosphate kinase
HMPP kinase

Gene Name:

thiD

Enzyme Class:

Biological Properties

General Function:

Involved in phosphomethylpyrimidine kinase activity

Specific Function:

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine

Cellular Location:

Not Available

SMPDB Pathways:

Thiamin diphosphate biosynthesis PW002028

KEGG Pathways:

Metabolic pathways eco01100
Thiamine metabolism ec00730

KEGG Reactions:

1.0

↔

1.0

1.04-Amino-5-hydroxymethyl-2-methylpyrimidine + 1.0Adenosine triphosphate ↔ 1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.0ADP + 1.0Hydrogen ion + 1.04-amino-5-phosphonooxymethyl-2-methylpyrimidine
ReactionCard

1.0

→

1.0

1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.0Adenosine triphosphate → 1.02-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 1.0ADP
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1.0

↔

1.0

1.0Adenosine triphosphate + 1.04-Amino-5-hydroxymethyl-2-methylpyrimidine ↔ 1.0ADP + 1.04-Amino-2-methyl-5-phosphomethylpyrimidine
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.04-Amino-2-methyl-5-phosphomethylpyrimidine ↔ 1.0ADP + 1.02-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate
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SMPDB Reactions:

1.04-amino-2-methyl-5-phosphomethylpyrimidine

1.0

→

1.0

1.04-amino-2-methyl-5-phosphomethylpyrimidine + 1.0Adenosine triphosphate → 1.0ADP + 1.02-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

→

1.0

1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.0Adenosine triphosphate → 1.02-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 1.0ADP
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.04-Amino-5-hydroxymethyl-2-methylpyrimidine → 1.0ADP + 1.04-Amino-2-methyl-5-phosphomethylpyrimidine
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB20046	2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate	MetaboCard
ECMDB20084	4-Amino-2-methyl-5-phosphomethylpyrimidine	MetaboCard
ECMDB20086	4-Amino-5-hydroxymethyl-2-methylpyrimidine	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Function
catalytic activity
kinase activity
phosphomethylpyrimidine kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process

Gene Properties

Blattner:

b2103

Gene Orientation

Counterclockwise

Centisome Percentage:

47.02

Left Sequence End

2181738

Right Sequence End

2182538

Gene Sequence:

>801 bp
GTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTA
CCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACC
GAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTG
TCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACA
ACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCGCCGATCAGGTACCCGCT
TTCGTGCTGCCACAACATCCTTCTGGCGTAATGACCCTTTCGGCGGCACATCCAGTGTAT
TGCTGCCCGTTACGTGAACGAGAACCCATTTTGCGTCTGGCGAAGTCGGCACTGGTGACG
GTCTATCCATTACCTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTC
AGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCAC
CACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAAC
GCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAG
CGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGT
GAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGT
CCCGGCAAGCCTGATAAATAA

Protein Properties

Pfam Domain Function:

Phos_pyr_kin (PF08543 )

Protein Residues:

266

Protein Molecular Weight:

28633

Protein Theoretical pI:

PDB File:

1JXI

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFV
AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS
AVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRSLLAMGCGAVLMKGGHL
DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL
SSALAQADTLEVGHGIGPVHHFHAWW

References

External Links:

Resource	Link
Uniprot ID:	P76422
Uniprot Name:	THID_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674383
PDB ID:	1JXI
Ecogene ID:	EG14068
Ecocyc:	EG14068
ColiBase:	b2103
Kegg Gene:	b2103
EchoBASE ID:	EB3821
CCDB:	THID_ECOLI
BacMap:	16130041

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
Mizote, T., Tsuda, M., Smith, D. D., Nakayama, H., Nakazawa, T. (1999). "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase." Microbiology 145 ( Pt 2):495-501. Pubmed: 10075431