Identification
Name:Beta-hexosaminidase
Synonyms:
  • Beta-N-acetylhexosaminidase
  • N-acetyl-beta-glucosaminidase
Gene Name:nagZ
Enzyme Class:
Biological Properties
General Function:Involved in beta-N-acetylhexosaminidase activity
Specific Function:Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides
Cellular Location:Cytoplasm
SMPDB Pathways:
  • 1,6-anhydro-<i>N</i>-acetylmuramic acid recycling PW002064
  • Amino sugar and nucleotide sugar metabolism I PW000886
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • Amino sugar and nucleotide sugar metabolism III PW000895
KEGG Pathways:
  • Amino sugar and nucleotide sugar metabolism ec00520
  • Glycosaminoglycan degradation ec00531
  • Glycosphingolipid biosynthesis - ganglio series ec00604
  • Glycosphingolipid biosynthesis - globo series ec00603
  • Metabolic pathways eco01100
  • Other glycan degradation ec00511
KEGG Reactions:
1.0Thumb+1.0Thumb2.0Thumb
1.0Thumb+1.0Globoside1.0Thumb+1.0Globotriaosylceramide
1.0Water + 1.0Globoside ↔ 1.0N-Acetyl-b-D-galactosamine + 1.0Globotriaosylceramide
ReactionCard
1.0GM2+1.0Thumb1.0GM3+1.0Thumb
1.0GM2 + 1.0Water ↔ 1.0GM3 + 1.0N-Acetyl-b-D-galactosamine
ReactionCard
1.0G13033+1.0Thumb1.0G08421+1.0Thumb
1.0G13033 + 1.0Water ↔ 1.0G08421 + 1.0N-Acetyl-b-D-galactosamine
ReactionCard
1.0G06780+1.0Thumb1.0G08421+1.0Thumb
1.0G06780 + 1.0Water ↔ 1.0G08421 + 1.0N-Acetyl-b-D-galactosamine
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Chitobiose+1.0Thumb2.0N-Acetyl-D-glucosamine+2.0Thumb
1.0Water + 1.0Chitobiose + 1.0Chitobiose → 2.0N-Acetyl-D-glucosamine + 2.0N-Acetylglucosamine
ReactionCard
1.0N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate+1.0Thumb1.0Thumb+1.0anhydro-n-acetylmuramic acid
1.0N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate + 1.0Water → 1.0N-Acetylglucosamine + 1.0anhydro-n-acetylmuramic acid
ReactionCard
EcoCyc Reactions:
1.0an N-acetyl-β-D-hexosaminide+1.0Thumb ? 1.0an organic molecule+1.0an N-acetyl-β-D-hexosamine
1.0an N-acetyl-β-D-hexosaminide + 1.0Water ? 1.0an organic molecule + 1.0an N-acetyl-β-D-hexosamine
ReactionCard
1.0N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate+1.0Thumb ? 1.0Thumb+1.0Thumb
1.0N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate + 1.0Water ? 1.0N-Acetyl-D-glucosamine + 1.01,6-Anhydro-N-acetylmuramate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB205191,6-Anhydro-N-acetylmuramateMetaboCard
ECMDB211111,6-Anhydrous-N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB211121,6-Anhydrous-N-Acetylmuramyl-tripeptideMetaboCard
ECMDB03556ChitobioseMetaboCard
ECMDB00853N-Acetyl-b-D-galactosamineMetaboCard
ECMDB00215N-Acetyl-D-glucosamineMetaboCard
ECMDB21248N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acidMetaboCard
ECMDB21249N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB21250N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
ECMDB21561N-AcetylglucosamineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b1107
Gene OrientationClockwise
Centisome Percentage:25.07
Left Sequence End1163318
Right Sequence End1164343
Gene Sequence:
>1026 bp
ATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGAT
GGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACG
TTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTC
ATGACCCAGGACGATCTTCCTTTCGCGAAAAGTGCCGCACTGGTAGTGAAGAATCCCTAC
CTGACTTACGCGCGCATGGCGCAAATTTTAGATACCACGCCGCAGCCCGCGCAGAACATT
GCACCCAGTGCGGTGATCGACGCGACGGCGAAGCTGGGTAACAACGTATCGATTGGCGCT
AACGCGGTGATTGAGTCCGGCGTTGAACTGGGCGATAACGTGATTATCGGTGCCGGTTGC
TTCGTAGGTAAAAACAGCAAAATCGGTGCAGGTTCGCGTCTCTGGGCGAACGTAACCATT
TACCATGAGATCCAGATCGGTCAGAATTGCCTGATCCAGTCCGGAACAGTGGTAGGCGCA
GACGGCTTTGGTTATGCCAACGATCGTGGTAACTGGGTGAAGATCCCACAGATTGGTCGC
GTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTG
GATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGTGCCAGATTGCACATAAC
GTCGTGATTGGCGACAATACGGCGGTTGCCGGTGGCGTCATTATGGCGGGCAGCCTGAAA
ATTGGTCGTTACTGCATGATCGGCGGAGCCAGCGTAATCAACGGGCATATGGAAATATGC
GACAAAGTGACGGTTACGGGCATGGGTATGGTGATGCGTCCCATCACTGAACCAGGCGTC
TATTCCTCAGGCATTCCGCTGCAACCCAACAAAGTCTGGCGCAAAACCGCTGCACTGGTG
ATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAA
GACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:341
Protein Molecular Weight:37594
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Beta-hexosaminidase
MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRELVRQIRAASRNRLVVA
VDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGWLMASEMIAMDIDISFAPV
LDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKTTGKHFPGHGAVTADSHKETP
CDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSDVDPRPASGSPYWLKTVLRQELG
FDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMILVCNNRKGAVSVLDNLSPIKAERV
TRLYHKGSFSRQELMDSARWKAISTRLNQLHERWQEEKAGH
References
External Links:
ResourceLink
Uniprot ID:P75949
Uniprot Name:NAGZ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902920
Ecogene ID:EG13433
Ecocyc:EG13433
ColiBase:b1107
Kegg Gene:b1107
EchoBASE ID:EB3207
CCDB:NAGZ_ECOLI
BacMap:16129070
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cheng, Q., Li, H., Merdek, K., Park, J. T. (2000). "Molecular characterization of the beta-N-acetylglucosaminidase of Escherichia coli and its role in cell wall recycling." J Bacteriol 182:4836-4840. Pubmed: 10940025
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Yem, D. W., Wu, H. C. (1976). "Purification and properties of beta-N-acetylglucosaminidase from Escherichia coli." J Bacteriol 125:324-331. Pubmed: 1377