Putative isochorismatase family protein rutB (P75897)

Identification
Name:Putative isochorismatase family protein rutB
Synonyms:
  • Pyrimidine utilization protein B
Gene Name:rutB
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
Specific Function:Makes part of the rut operon, which is required for the utilization of pyrimidines as sole nitrogen source
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.0Ureidoacrylate peracid → 1.0Carbamic acid + 1.0Hydrogen ion + 1.0Peroxyaminoacrylate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Ureidoacrylate peracid + 1.0Water ↔ 1.0Peroxyaminoacrylate + 1.0Carbamic acid
ReactionCard
1.0Ureidoacrylate+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Ureidoacrylate + 1.0Water ↔ 1.03-Aminoacrylate + 1.0Carbamic acid
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Ureidoacrylate peracid + 1.0Water + 1.0Carbamic acid + 1.0(Z)-2-Methyl-ureidoacrylate peracid ↔ 1.0Peroxyaminoacrylate + 1.0Carbon dioxide + 1.0Ammonia + 1.0(Z)-2-Methyl-peroxyaminoacrylate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Ureidoacrylate peracid + 1.0Water → 1.0Peroxyaminoacrylate + 1.0Carbamic acid + 1.0Hydrogen ion + 1.03-Aminoacrylate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Water + 1.0Ureidoacrylate peracid → 1.0Carbamic acid + 1.0Hydrogen ion + 1.0Peroxyaminoacrylate
ReactionCard
1.03-ureidoacrylate+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.03-ureidoacrylate + 1.0Water → 1.0Hydrogen ion + 1.0Carbamic acid + 1.03-Aminoacrylate
ReactionCard
1.0(Z)-2-methylureidoacrylate peracid+1.0Thumb → 1.0(Z)-2-methyl-peroxyaminoacrylate+1.0Thumb+1.0Thumb
1.0(Z)-2-methylureidoacrylate peracid + 1.0Water → 1.0(Z)-2-methyl-peroxyaminoacrylate + 1.0Carbamic acid + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Ureidoacrylate peracid + 1.0Water → 1.0Peroxyaminoacrylate + 1.0Ammonia
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0(Z)-3-Ureidoacrylate + 1.0Water → 1.03-Aminoacrylate + 1.0Carbon dioxide + 1.0Ammonia
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0(Z)-2-Methyl-ureidoacrylate peracid + 1.0Water → 1.0(Z)-2-Methyl-peroxyaminoacrylate + 1.0Carbon dioxide + 1.0Ammonia
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23125(Z)-2-Methyl-peroxyaminoacrylateMetaboCard
ECMDB23105(Z)-2-Methyl-ureidoacrylate peracidMetaboCard
ECMDB23127(Z)-3-UreidoacrylateMetaboCard
ECMDB211673-AminoacrylateMetaboCard
ECMDB00051AmmoniaMetaboCard
ECMDB03551Carbamic acidMetaboCard
ECMDB04030Carbon dioxideMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21275PeroxyaminoacrylateMetaboCard
ECMDB21299Ureidoacrylate peracidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
Process
metabolic process
Gene Properties
Blattner:b1011
Gene OrientationCounterclockwise
Centisome Percentage:23.09
Left Sequence End1071394
Right Sequence End1072086
Gene Sequence:
>693 bp
ATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGC
GCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCT
AAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAATTCG
TGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTT
GTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCA
GCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAAT
GCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTC
CGTCCAATTCAATTACAAGAATTACCCGCAGAAGCGCCGGACACACTCAAGTTTTCGCGA
TCCGGTAACTATATCAATGTTCATAATCCATCACCTTTTTATGTCACCCTGGTTAACTTA
CAAGTGGGCAGCCAAAAGTTGGGGAATGCTATGGCTGCACCCAGAGTTAATTCACAAATT
CCCTTACCCTCAGGAGTGCAGGGAAAGCTGAAATTTCAGACCGTTAATGATTATGGTTCA
GTAACTCCGGTCAGAGAAGTGAACTTAAACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:230
Protein Molecular Weight:25209
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Putative isochorismatase family protein rutB
MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
RAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
ELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
GVVLEDATHQAGPKFAQKAALFNIETFFGWVSDVETFCDALSPTSFAHIA
References
External Links:
ResourceLink
Uniprot ID:P75897
Uniprot Name:RUTB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674668
Ecogene ID:EG13858
Ecocyc:EG13858
ColiBase:b1011
Kegg Gene:b1011
EchoBASE ID:EB3618
CCDB:RUTB_ECOLI
BacMap:90111203
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Loh, K. D., Gyaneshwar, P., Markenscoff Papadimitriou, E., Fong, R., Kim, K. S., Parales, R., Zhou, Z., Inwood, W., Kustu, S. (2006). "A previously undescribed pathway for pyrimidine catabolism." Proc Natl Acad Sci U S A 103:5114-5119. Pubmed: 16540542
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232