ECMDB

Identification

Name:

Phosphatase ybjI

Synonyms:

Not Available

Gene Name:

ybjI

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

KEGG Reactions:

1.0

↔

1.0

1.0Flavin Mononucleotide + 1.0Water ↔ 1.0Riboflavin + 1.0Phosphate
ReactionCard

SMPDB Reactions:

1.05-Amino-6-(5'-phosphoribitylamino)uracil

1.0

→

1.0

1.05-Amino-6-(5'-phosphoribitylamino)uracil + 1.0Water + 1.05-Amino-6-(5'-phosphoribitylamino)uracil → 1.0Phosphate + 1.05-Amino-6-ribitylamino uracil
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Flavin Mononucleotide + 1.0Water ↔ 1.0Riboflavin + 1.0Phosphate
ReactionCard

1.0

→

1.0

1.0Flavin Mononucleotide + 1.0Water → 1.0Riboflavin + 1.0Phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21177	5-Amino-6-(5'-phosphoribitylamino)uracil	MetaboCard
ECMDB04145	5-Amino-6-ribitylamino uracil	MetaboCard
ECMDB01520	Flavin Mononucleotide	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB00244	Riboflavin	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
hydrolase activity
Process
metabolic process

Gene Properties

Blattner:

b0844

Gene Orientation

Counterclockwise

Centisome Percentage:

19.06

Left Sequence End

884539

Right Sequence End

885354

Gene Sequence:

>816 bp
ATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTT
TGGGGCGTAGTGTTAGGCCTGTTAATTGGCCATATGTTTGATAAAGCCCGTAGCCGTAAA
ATGGCGTGGTTCGCCAACCAGCGTGAGCGTCAGGCGCTGTTTTTTGCCACCACTTTTGAA
GTGATGGGGCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCC
AGCCAGTTGATGGACCGAATGAATCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCG
TTCCGGGTGGGAAAATCAGACAATTACCCGCTGCGCGAAAAGATGCGCCAGTTTCGCAGT
GTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCG
TTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCAGAAGAA
TTAGGGATCTCCCGCGCTCAGTTTGACCAGTTTTTGCGCATGATGCAGGGCGGTGCACAG
TTTGGCGGCGGTTATCAGCAGCAAACTGGCGGTGGTAACTGGCAGCAAGCGCAGCGTGGC
CCAACGCTGGAAGATGCCTGTAATGTGCTGGGCGTGAAGCCGACGGATGATGCGACCACC
ATCAAACGTGCCTACCGTAAGCTGATGAGTGAACACCATCCCGATAAGCTGGTGGCGAAA
GGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCA
TATGAGCTGATAAAGCAGCAGAAAGGGTTTAAATGA

Protein Properties

Pfam Domain Function:

Hydrolase (PF00702 )

Protein Residues:

271

Protein Molecular Weight:

30196

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Phosphatase ybjI
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIAN
EIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDD
AMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVSVHTGN
GSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
AAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ

References

External Links:

Resource	Link
Uniprot ID:	P75809
Uniprot Name:	YBJI_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21321936
Ecogene ID:	EG13678
Ecocyc:	EG13678
ColiBase:	b0844
Kegg Gene:	b0844
EchoBASE ID:	EB3442
CCDB:	YBJI_ECOLI
BacMap:	90111176

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Fountoulakis, M., Takacs, M. F., Berndt, P., Langen, H., Takacs, B. (1999). "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Electrophoresis 20:2181-2195. Pubmed: 10493123
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Kuznetsova, E., Proudfoot, M., Sanders, S. A., Reinking, J., Savchenko, A., Arrowsmith, C. H., Edwards, A. M., Yakunin, A. F. (2005). "Enzyme genomics: Application of general enzymatic screens to discover new enzymes." FEMS Microbiol Rev 29:263-279. Pubmed: 15808744
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232

Phosphatase ybjI (P75809)