Glutathione import ATP-binding protein gsiA (P75796)

Identification
Name:Glutathione import ATP-binding protein gsiA
Synonyms:Not Available
Gene Name:gsiA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex gsiABCD involved in glutathione import. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Glutathione → 1.0ADP + 1.0Glutathione + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00125GlutathioneMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
Process
establishment of localization
peptide transport
transport
Gene Properties
Blattner:b0829
Gene OrientationClockwise
Centisome Percentage:18.68
Left Sequence End866743
Right Sequence End868614
Gene Sequence:
>1872 bp
ATGATGGACAGCTTACGCACGGCTGCAAACAGTCTCGTGCTCAAGATTATTTTCGGTATC
ATTATCGTGTCGTTCATATTGACCGGCGTGAGTGGTTACCTGATTGGCGGAGGCAATAAC
TACGCCGCAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAAC
AGCGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAAC
GAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTG
CTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCG
ATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCGCTATAACGGT
ATCCTCAACCAGATGGGGATGACCGCCGATCAGTACGCCCAGGCGCTGCGTAACCAGCTC
ACTACCCAACAGCTGATTAACGGCGTTGCCGGTACCGATTTTATGCTGAAAGGTGAAACC
GACGAGCTGGCGGCACTGGTCGCGCAACAACGCGTGGTGCGTGAGGCGACTATCGATGTT
AACGCGCTGGCGGCGAAGCAGCCTGTGACCGAACAGGAGATTGCCAGCTACTACGAACAA
AACAAAAACAATTTCATGACGCCGGAACAATTCCGCGTGAGTTACATCAAGCTGGATGCC
GCAACGATGCAGCAACCGGTTAGCGATGCGGATATCCAGAGCTACTACGACCAGCATCAG
GATCAATTCACCCAGCCGCAGCGTACCCGCTACAGCATCATCCAGACCAAAACTGAAGAT
GAAGCGAAAGCGGTACTTGATGAGCTGAATAAAGGCGGTGATTTTGCTGCATTAGCCAAA
GAAAAATCTGCCGATATTATCTCTGCTCGTAACGGCGGCGATATGGGTTGGTTAGAAGAT
GCCACTATCCCGGACGAACTGAAAAATGCTGGTCTGAAAGAAAAAGGCCAACTGTCTGGT
GTCATCAAATCTTCGGTCGGTTTCCTGATTGTACGTCTGGACGACATTCAGCCAGCGAAA
GTGAAATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCC
CTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAG
TCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGC
AAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGC
GGTCTGGTAGGTGAAAACGGCGCGCCGGGCATCAACTCTGACATCATCACCGTAGACGGC
GACCGCGCATTCGTGCTGCGCATCAGCGAGCACAAACCGGAAGCGGTGAAACCGTTGGCA
GATGTTCAGGAACAAGTTAAGGCATTGGTTCAGCACAACAAAGCTGAACAACAGGCGAAA
GTGGATGCTGAGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAG
GCTGCCGGTCTGAAATTTGGCGAGCCGAAAACCTTAAGCCGTTCCGGTCGTGACCCGATT
AGCCAGGCGGCGTTTGCACTGCCACTGCCAGCGAAAGACAAACCGAGCTACGGTATGGCG
ACCGATATGCAAGGTAATGTGGTTCTGCTGGCGCTGGATGAAGTGAAACAAGGTTCAATG
CCGGAAGATCAGAAAAAAGCGATGGTGCAGGGTATCACCCAGAACAACGCACAAATCGTC
TTTGAAGCTCTGATGAGTAACCTGCGTAAAGAGGCGAAAATCAAAATTGGCGATGCGCTG
GAACAGCAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:623
Protein Molecular Weight:69113
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glutathione import ATP-binding protein gsiA
MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGETLAIVGESGSGKSVTAL
ALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQNAAQMRHVRGADMAMIFQEPMTSLNPV
FTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQTILSRYPHQLSGGMRQRVMIA
MALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEMSMGVIFITHDMGVVAEIADRVL
VMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLGAMKGLDYPRRFPLISLEHPAKQAP
PIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVTREVHAVEKVSFDLWPGETLSLVGESG
SGKSTTGRALLRLVESQGGEIIFNGQRIDTLSPGKLQALRRDIQFIFQDPYASLDPRQTI
GDSIIEPLRVHGLLPGKDAAARVAWLLERVGLLPEHAWRYPHEFSGGQRQRICIARALAL
NPKVIIADEAVSALDVSIRGQIINLLLDLQRDFGIAYLFISHDMAVVERISHRVAVMYLG
QIVEIGPRRAVFENPQHPYTRKLLAAVPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAV
SLQCVGPGHYVAQPQSEYAFMRR
References
External Links:
ResourceLink
Uniprot ID:P75796
Uniprot Name:GSIA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674581
Ecogene ID:EG13472
Ecocyc:EG13472
ColiBase:b0829
Kegg Gene:b0829
EchoBASE ID:EB3245
CCDB:GSIA_ECOLI
BacMap:145698235
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Suzuki, H., Koyanagi, T., Izuka, S., Onishi, A., Kumagai, H. (2005). "The yliA, -B, -C, and -D genes of Escherichia coli K-12 encode a novel glutathione importer with an ATP-binding cassette." J Bacteriol 187:5861-5867. Pubmed: 16109926