Cytochrome c-type biogenesis protein CcmE (P69490)

Identification
Name:Cytochrome c-type biogenesis protein CcmE
Synonyms:
  • Cytochrome c maturation protein E
  • Heme chaperone CcmE
Gene Name:ccmE
Enzyme Class:Not Available
Biological Properties
General Function:Involved in protein-heme linkage
Specific Function:Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH
Cellular Location:Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential).
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Heme → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Heme
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB03178HemeMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
plasma membrane
Process
cellular component assembly
cellular component organization
cellular component organization or biogenesis
cellular protein complex assembly
cytochrome complex assembly
macromolecular complex assembly
macromolecule metabolic process
macromolecule modification
metabolic process
post-translational protein modification
protein complex assembly
protein modification process
protein-heme linkage
protein-tetrapyrrole linkage
Gene Properties
Blattner:b2197
Gene OrientationCounterclockwise
Centisome Percentage:49.42
Left Sequence End2292923
Right Sequence End2293402
Gene Sequence:
>480 bp
ATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATG
CCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTG
ATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATT
ATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAA
GCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTAT
GAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAA
GGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTC
CACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:159
Protein Molecular Weight:17698
Protein Theoretical pI:7
PDB File:1SR3
Signaling Regions:
  • None
Transmembrane Regions:
  • 9-29
Protein Sequence:
>Cytochrome c-type biogenesis protein CcmE
MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEILYGKRETQQMPEVGQRL
RVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDLFREGQGVVVQGELEKGNH
ILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS
References
External Links:
ResourceLink
Uniprot ID:P69490
Uniprot Name:CCME_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321929
PDB ID:1SR3
Ecogene ID:EG12055
Ecocyc:EG12055
ColiBase:b2197
Kegg Gene:b2197
EchoBASE ID:EB1986
CCDB:CCME_ECOLI
BacMap:16130134
General Reference:
  • Ahuja, U., Thony-Meyer, L. (2005). "CcmD is involved in complex formation between CcmC and the heme chaperone CcmE during cytochrome c maturation." J Biol Chem 280:236-243. Pubmed: 15513913
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Enggist, E., Schneider, M. J., Schulz, H., Thony-Meyer, L. (2003). "Biochemical and mutational characterization of the heme chaperone CcmE reveals a heme binding site." J Bacteriol 185:175-183. Pubmed: 12486054
  • Enggist, E., Thony-Meyer, L., Guntert, P., Pervushin, K. (2002). "NMR structure of the heme chaperone CcmE reveals a novel functional motif." Structure 10:1551-1557. Pubmed: 12429096
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ren, Q., Ahuja, U., Thony-Meyer, L. (2002). "A bacterial cytochrome c heme lyase. CcmF forms a complex with the heme chaperone CcmE and CcmH but not with apocytochrome c." J Biol Chem 277:7657-7663. Pubmed: 11744735
  • Ren, Q., Thony-Meyer, L. (2001). "Physical interaction of CcmC with heme and the heme chaperone CcmE during cytochrome c maturation." J Biol Chem 276:32591-32596. Pubmed: 11384983
  • Schulz, H., Hennecke, H., Thony-Meyer, L. (1998). "Prototype of a heme chaperone essential for cytochrome c maturation." Science 281:1197-1200. Pubmed: 9712585
  • Thony-Meyer, L., Fischer, F., Kunzler, P., Ritz, D., Hennecke, H. (1995). "Escherichia coli genes required for cytochrome c maturation." J Bacteriol 177:4321-4326. Pubmed: 7635817