Glucose-1-phosphate thymidylyltransferase 2 (P61887)

Identification
Name:Glucose-1-phosphate thymidylyltransferase 2
Synonyms:
  • G1P-TT 2
  • dTDP-glucose pyrophosphorylase 2
  • dTDP-glucose synthase 2
Gene Name:rmlA2
Enzyme Class:
Biological Properties
General Function:Involved in glucose-1-phosphate thymidylyltransferase activity
Specific Function:Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary Metabolites: enterobacterial common antigen biosynthesis PW000959
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 3 PW002046
  • Starch and sucrose metabolism PW000941
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Thymidine 5'-triphosphate + 1.0Glucose 1-phosphate + 1.0Hydrogen ion ↔ 1.0dTDP-D-Glucose + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Thymidine 5'-triphosphate + 1.0Glucose 1-phosphate ↔ 1.0Pyrophosphate + 1.0dTDP-D-Glucose
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Glucose 1-phosphate + 1.0Thymidine 5'-triphosphate + 1.0Hydrogen ion → 1.0Pyrophosphate + 1.0TDP-Glucose
ReactionCard
1.0Alpha-D-glucose 1-phosphate+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Alpha-D-glucose 1-phosphate + 1.0Thymidine 5'-triphosphate + 1.0Hydrogen ion → 1.0Pyrophosphate + 1.0dTDP-D-Glucose
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Thymidine 5'-triphosphate + 1.0Glucose 1-phosphate + 1.0Hydrogen ion ↔ 1.0dTDP-D-Glucose + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01328dTDP-D-GlucoseMetaboCard
ECMDB01586Glucose 1-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB20298TDP-GlucoseMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
GO Classification:
Function
catalytic activity
glucose-1-phosphate thymidylyltransferase activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
carbohydrate metabolic process
cellular polysaccharide biosynthetic process
extracellular polysaccharide biosynthetic process
metabolic process
polysaccharide biosynthetic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b3789
Gene OrientationClockwise
Centisome Percentage:85.60
Left Sequence End3971631
Right Sequence End3972512
Gene Sequence:
>882 bp
ATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTG
AATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGAC
GAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGTGCTACTGGCTGGGCGAACTTCAGC
CGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGT
TGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATC
CTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATG
GGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTG
CTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATT
AAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATGGAT
ATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAAT
ATGCTGTCGCGCCGCGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAA
CAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGCGCAAACCCTGATTGTCTGG
GGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCC
GGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGAC
GCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:293
Protein Molecular Weight:32734
Protein Theoretical pI:5
PDB File:1MC3
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glucose-1-phosphate thymidylyltransferase 2
MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLAGIREILIITTPEDKGY
FQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPSCLVLGDNIFFGQGFSPKL
RHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEKPKQPKSNWAVTGLYFYDSKV
VEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRGFAWLDTGTHDSLIEASTFVQTV
EKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKTGYGQYLLELLRARPRQY
References
External Links:
ResourceLink
Uniprot ID:P61887
Uniprot Name:RMLA2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674491
PDB ID:1MC3
Ecogene ID:EG11454
Ecocyc:EG11454
ColiBase:b3789
Kegg Gene:b3789
EchoBASE ID:EB1423
CCDB:RMLA2_ECOLI
BacMap:16131645
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Marolda, C. L., Valvano, M. A. (1995). "Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene." J Bacteriol 177:5539-5546. Pubmed: 7559340
  • Sivaraman, J., Sauve, V., Matte, A., Cygler, M. (2002). "Crystal structure of Escherichia coli glucose-1-phosphate thymidylyltransferase (RffH) complexed with dTTP and Mg2+." J Biol Chem 277:44214-44219. Pubmed: 12171937