6,7-dimethyl-8-ribityllumazine synthase (P61714)

Identification
Name:6,7-dimethyl-8-ribityllumazine synthase
Synonyms:
  • DMRL synthase
  • Lumazine synthase
  • Riboflavin synthase beta chain
Gene Name:ribH
Enzyme Class:
Biological Properties
General Function:Involved in riboflavin biosynthetic process
Specific Function:Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino- 2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4- phosphate yielding 6,7-dimethyl-8-lumazine
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
2.0Thumb → 1.0Thumb+1.0Thumb
2.06,7-Dimethyl-8-(1-D-ribityl)lumazine → 1.05-Amino-6-ribitylamino uracil + 1.0Riboflavin
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb+1.0Thumb
1.05-Amino-6-ribitylamino uracil + 1.03,4-Dihydroxy-2-butanone-4-P → 1.06,7-Dimethyl-8-(1-D-ribityl)lumazine + 2.0Water + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+2.0Thumb+1.0Thumb
1.05-Amino-6-ribitylamino uracil + 1.03,4-Dihydroxy-2-butanone-4-P ↔ 1.06,7-Dimethyl-8-(1-D-ribityl)lumazine + 2.0Water + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB040603,4-Dihydroxy-2-butanone-4-PMetaboCard
ECMDB230165-amino-6-(D-ribitylamino)uracilMetaboCard
ECMDB041455-Amino-6-ribitylamino uracilMetaboCard
ECMDB038266,7-Dimethyl-8-(1-D-ribityl)lumazineMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB23121L-3,4-Dihydroxybutan-2-one 4-phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00244RiboflavinMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
macromolecular complex
protein complex
riboflavin synthase complex
Process
metabolic process
nitrogen compound metabolic process
riboflavin biosynthetic process
riboflavin metabolic process
Gene Properties
Blattner:b0415
Gene OrientationClockwise
Centisome Percentage:9.35
Left Sequence End433871
Right Sequence End434341
Gene Sequence:
>471 bp
ATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGCCATTTTGCTC
AGCAAAGCTAAAAAAGATCTCTCATTTGCCGAGATTGCCGACGGCACCGGTCTGGCAGAA
GCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTG
GTCGGGGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTG
CGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATG
TTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGAGAAATTTGGCGATGGCATT
ATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAA
CGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAACCGTTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:156
Protein Molecular Weight:16156
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>6,7-dimethyl-8-ribityllumazine synthase
MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQVKDENITVVWVPGAYEL
PLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLAHVAQDSEIPVAFGVLTTE
SIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA
References
External Links:
ResourceLink
Uniprot ID:P61714
Uniprot Name:RISB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674482
Ecogene ID:EG11322
Ecocyc:EG11322
ColiBase:b0415
Kegg Gene:b0415
EchoBASE ID:EB1298
CCDB:RISB_ECOLI
BacMap:16128400
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Mortl, S., Fischer, M., Richter, G., Tack, J., Weinkauf, S., Bacher, A. (1996). "Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli." J Biol Chem 271:33201-33207. Pubmed: 8969176
  • Taura, T., Ueguchi, C., Shiba, K., Ito, K. (1992). "Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation." Mol Gen Genet 234:429-432. Pubmed: 1406588