D-lactate dehydrogenase_ (P52643)

Identification
Name:D-lactate dehydrogenase_
Synonyms:
  • D-LDH
  • Fermentative lactate dehydrogenase
Gene Name:ldhA
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Specific Function:Fermentative lactate dehydrogenase
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
  • Microbial metabolism in diverse environments ec01120
  • Pyruvate metabolism ec00620
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Lactic acid + 1.0NAD ↔ 1.0Hydrogen ion + 1.0NADH + 1.0Pyruvic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Lactic acid + 1.0NAD ↔ 1.0Hydrogen ion + 1.0NADH + 1.0Pyruvic acid
ReactionCard
1.0Thumb+1.0Thumb ← 1.0Thumb+1.0Thumb+1.0Thumb
1.0NAD + 1.0D-Lactic acid ← 1.0Hydrogen ion + 1.0NADH + 1.0Pyruvic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01311D-Lactic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
NAD or NADH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process
metabolic process
Gene Properties
Blattner:b1380
Gene OrientationCounterclockwise
Centisome Percentage:31.03
Left Sequence End1439878
Right Sequence End1440867
Gene Sequence:
>990 bp
ATGGCTTCACAACTGACTGATGCATTTGCGCGTAAGTTTTACTACTTGCGCCTGTCGATT
ACCGATGTGTGTAACTTTCGTTGCACCTACTGCCTGCCGGATGGCTACAAACCGAGCGGC
GTCACCAATAAAGGCTTTCTTACCGTCGATGAAATTCGCCGGGTTACGCGCGCCTTCGCC
AGACTGGGCACCGAAAAAGTGCGCCTGACAGGAGGAGAGCCGTCTTTACGCCGCGACTTT
ACCGATATCATCGCCGCTGTGCGGGAAAACGACGCTATCCGCCAGATTGCGGTCACAACC
AATGGTTACCGTCTGGAACGCGATGTGGCGAGCTGGCGCGATGCGGGACTTACTGGCATT
AACGTCAGTGTCGACAGTCTGGACGCCCGCCAGTTTCACGCTATTACCGGGCAGGATAAA
TTCAACCAGGTCATGGCAGGGATTGATGCTGCATTTGAGGCCGGTTTTGAGAAGGTCAAA
GTCAATACCGTGCTGATGCGTGATGTTAATCATCACCAGCTCGACACCTTTCTGAACTGG
ATCCAGCATCGCCCTATCCAGCTGCGTTTCATCGAACTGATGGAAACGGGCGAGGGCAGC
GAGCTCTTCCGTAAGCATCACATCTCTGGTCAGGTTCTGCGTGACGAGCTACTGCGTCGC
GGCTGGATCCACCAATTACGTCAACGCAGCGACGGTCCCGCGCAAGTCTTTTGCCATCCA
GATTACGCCGGAGAGATTGGCCTTATCATGCCGTATGAAAAAGACTTCTGCGCCACTTGC
AACCGCCTGCGCGTTTCCTCCATTGGTAAACTCCATCTCTGCCTGTTTGGTGAAGGCGGC
GTTAACCTGCGCGATCTGCTGGAAGACGATACCCAGCAACAGGCGCTGGAAGCGCGTATT
TCAGCGGCGCTGCGGGAGAAGAAACAGACCCATTTCCTGCATCAAAACAACACCGGTATT
ACGCAAAACTTATCGTACATTGGCGGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:329
Protein Molecular Weight:36535
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>D-lactate dehydrogenase
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
ALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFEQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
AEALTSISQTTLQNLSNLEKGETCPNELV
References
External Links:
ResourceLink
Uniprot ID:P52643
Uniprot Name:LDHD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651354
Ecogene ID:EG13186
Ecocyc:EG13186
ColiBase:b1380
Kegg Gene:b1380
EchoBASE ID:EB2978
CCDB:LDHD_ECOLI
BacMap:16129341
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bunch, P. K., Mat-Jan, F., Lee, N., Clark, D. P. (1997). "The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli." Microbiology 143 ( Pt 1):187-195. Pubmed: 9025293
  • Chuang, S. E., Blattner, F. R. (1993). "Characterization of twenty-six new heat shock genes of Escherichia coli." J Bacteriol 175:5242-5252. Pubmed: 8349564
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553