Nucleoside triphosphatase nudI (P52006)

Identification
Name:Nucleoside triphosphatase nudI
Synonyms:Not Available
Gene Name:nudI
Enzyme Class:Not Available
Biological Properties
General Function:Involved in hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
Specific Function:Catalyzes the hydrolysis of nucleoside triphosphates, with a preference for pyrimidine deoxynucleoside triphosphates (dUTP, dTTP and dCTP)
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Deoxyuridine triphosphate + 1.0Water ↔ 1.0dUMP + 1.0Hydrogen ion + 1.0Pyrophosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Deoxyuridine triphosphate + 1.0Water → 1.0Phosphate + 1.0Hydrogen ion + 1.0dUMP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0dCTP + 1.0Water → 1.0dCMP + 1.0Hydrogen ion + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Thymidine 5'-triphosphate + 1.0Water → 1.05-Thymidylic acid + 1.0Hydrogen ion + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Deoxyuridine triphosphate + 1.0Water ↔ 1.0dUMP + 1.0Hydrogen ion + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Deoxyuridine triphosphate + 1.0Water → 1.0Hydrogen ion + 1.0dUMP + 1.0Pyrophosphate
ReactionCard
Complex Reactions:
1.0Nucleoside triphosphate+1.0Thumb → 1.0nucleoside monophosphate+1.0Thumb
1.0Nucleoside triphosphate + 1.0Water → 1.0nucleoside monophosphate + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB012275-Thymidylic acidMetaboCard
ECMDB01202dCMPMetaboCard
ECMDB00998dCTPMetaboCard
ECMDB01191Deoxyuridine triphosphateMetaboCard
ECMDB01409dUMPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
Gene Properties
Blattner:b2251
Gene OrientationClockwise
Centisome Percentage:50.92
Left Sequence End2362576
Right Sequence End2363001
Gene Sequence:
>426 bp
GTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAAC
CGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCAT
GGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGA
TACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGC
CAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCAT
CTTGAGCGACAGTTCGCCGTAACGGAACCAAATCAGGTGTGGTGCGGTGATGTGACCTAT
AGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAA
TATTGA
Protein Properties
Pfam Domain Function:
Protein Residues:141
Protein Molecular Weight:16371
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Nucleoside triphosphatase nudI
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWAISGGGVEPGERIEEALRREIREELGE
QLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPE
DLVHYDLNVATRKTLRLKGLL
References
External Links:
ResourceLink
Uniprot ID:P52006
Uniprot Name:NUDI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674401
Ecogene ID:EG13275
Ecocyc:EG13275
ColiBase:b2251
Kegg Gene:b2251
EchoBASE ID:EB3060
CCDB:NUDI_ECOLI
BacMap:16130186
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jung, J., Ahn, Y. J., Kang, L. W. (2007). "Overexpression, crystallization and preliminary X-ray crystallographic analysis of Nudix hydrolase Orf141 from Escherichia coli K-1." Acta Crystallogr Sect F Struct Biol Cryst Commun 63:812-815. Pubmed: 17768363
  • Xu, W., Dunn, C. A., O'handley, S. F., Smith, D. L., Bessman, M. J. (2006). "Three new Nudix hydrolases from Escherichia coli." J Biol Chem 281:22794-22798. Pubmed: 16766526
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837