HMP-PP phosphatase (P46891)

Identification
Name:HMP-PP phosphatase
Synonyms:Not Available
Gene Name:cof
Enzyme Class:Not Available
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P). Can also hydrolyze other substrates such as MeO-HMP-PP and CF(3)-HMP-PP
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:Not Available
SMPDB Reactions:
1.0Thumb → 1.04-amino-2-methyl-5-diphosphomethylpyrimidine+1.0Thumb
1.0Pyridoxal 5'-phosphate → 1.04-amino-2-methyl-5-diphosphomethylpyrimidine + 1.02-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 1.0Water → 1.04-Amino-2-methyl-5-phosphomethylpyrimidine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Water + 1.0Pyridoxal 5'-phosphate → 1.0Phosphate + 1.0Pyridoxal
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200462-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphateMetaboCard
ECMDB200844-Amino-2-methyl-5-phosphomethylpyrimidineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01545PyridoxalMetaboCard
ECMDB01491Pyridoxal 5'-phosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
Process
metabolic process
Gene Properties
Blattner:b0446
Gene OrientationClockwise
Centisome Percentage:10.06
Left Sequence End466636
Right Sequence End467454
Gene Sequence:
>819 bp
ATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTA
GGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT
GCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG
TATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGT
GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCC
AGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG
CAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTC
GGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATC
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTC
GAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTA
GGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATGTTA
GTCAGCGTCGGTAGCGGATTTATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTC
CCGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCAC
TGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:272
Protein Molecular Weight:30371
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>HMP-PP phosphatase
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDA
YLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALL
QAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQLYEALGERAHLCFSATDCL
EVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLVSVGSGFIMGNAMPQLRAEL
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
References
External Links:
ResourceLink
Uniprot ID:P46891
Uniprot Name:COF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674586
Ecogene ID:EG13216
Ecocyc:EG13216
ColiBase:b0446
Kegg Gene:b0446
EchoBASE ID:EB3007
CCDB:COF_ECOLI
BacMap:90111136
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lawhorn, B. G., Gerdes, S. Y., Begley, T. P. (2004). "A genetic screen for the identification of thiamin metabolic genes." J Biol Chem 279:43555-43559. Pubmed: 15292217