ECMDB: N-methyl-L-tryptophan oxidase (P40874)

Identification

Name:

N-methyl-L-tryptophan oxidase

Synonyms:

MTOX

Gene Name:

solA

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in oxidoreductase activity

Specific Function:

Catalyzes the oxidative demethylation of N-methyl-L- tryptophan. Can also use other N-methyl amino acids, including sarcosine, which, however, is a poor substrate

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

EcoCyc Reactions:

1.0

→

1.0

1.0N-Methyltryptophan + 1.0Water + 1.0Oxygen → 1.0Formaldehyde + 1.0Hydrogen peroxide + 1.0L-Tryptophan
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Water + 1.0Oxygen + 1.0Sarcosine → 1.0Formaldehyde + 1.0Glycine + 1.0Hydrogen peroxide
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01426	Formaldehyde	MetaboCard
ECMDB00123	Glycine	MetaboCard
ECMDB21232	Hydrogen peroxide	MetaboCard
ECMDB00929	L-Tryptophan	MetaboCard
ECMDB21252	N-Methyltryptophan	MetaboCard
ECMDB04124	Oxygen	MetaboCard
ECMDB00271	Sarcosine	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
oxidoreductase activity

Gene Properties

Blattner:

b1059

Gene Orientation

Counterclockwise

Centisome Percentage:

24.11

Left Sequence End

1118691

Right Sequence End

1119809

Gene Sequence:

>1119 bp
ATGAGCCTGCCTTTTTTACGCACGCTGCAAGGCGATCGTTTTTTTCAGTTATTAATTCTT
GTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATC
GACTGGCACACCATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTA
AGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTGCGCCGCTTTGCTACGGAGCGTCGG
CTGGCGATGTTTATGGTGCTGGCGGCGGCGCTGCTTTCTACCTTTCTGACCAACGATGTC
GCGCTGTTTATTGTTGTTCCGCTGACTATCACGCTAAAAAGACTGTGTGAGATCCCGGTT
AATCGGCTGATTATTTTTGAGGCGCTGGCAGTCAACGCTGGTTCGCTACTGACGCCAATT
GGCAACCCGCAAAATATTCTTATCTGGGGACGTTCTGGTCTTTCGTTTGCCGGATTTATT
GCCCAAATGGCACCGCTGGCTGGCGCAATGATGCTGACGCTCCTGCTCCTGTGCTGGTGT
TGTTTCCCTGGAAAGGCGATGCAATACCATACGGGGGTGCAAACACCGGAGTGGAAACCG
CGGCTGGTGTGGAGTTGTCTGGGGCTGTATATCGTCTTTCTGACGGCGCTGGAGTTCAAA
CAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTG
GTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCAT
TTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAA
CCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGGTGATCAGTAACGTGCCGAGTACC
ATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGT
GGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGAT
CGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTG
GTGGGATATGTTTTGTTAGTTATACTCCCGGCCAACTAG

Protein Properties

Pfam Domain Function:

DAO (PF01266 )

Protein Residues:

372

Protein Molecular Weight:

40902

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>N-methyl-L-tryptophan oxidase
MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHHGDTRLIRHAYGEGEKY
VPLVLRAQTLWDELSRHNEEDPIFVRSGVINLGPADSTFLANVAHSAEQWQLNVEKLDAQ
GIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAKEAGCAQLFNCPVTAIRHDDD
GVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRKVFAWYQADGRYSVKNKFPAFTG
ELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERVPFAEVASDGSEAFPFLRNVLPGIG
CCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITGLSGHGFKFASVLGEIAADFAQDKKSD
FDLTPFRLSRFQ

References

External Links:

Resource	Link
Uniprot ID:	P40874
Uniprot Name:	MTOX_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062385
Ecogene ID:	EG12669
Ecocyc:	EG12669
ColiBase:	b1059
Kegg Gene:	b1059
EchoBASE ID:	EB2535
CCDB:	MTOX_ECOLI
BacMap:	16129022

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Ilari, A., Bonamore, A., Franceschini, S., Fiorillo, A., Boffi, A., Colotti, G. (2008). "The X-ray structure of N-methyltryptophan oxidase reveals the structural determinants of substrate specificity." Proteins 71:2065-2075. Pubmed: 18186483
Khanna, P., Schuman Jorns, M. (2001). "Characterization of the FAD-containing N-methyltryptophan oxidase from Escherichia coli." Biochemistry 40:1441-1450. Pubmed: 11170472
Khanna, P., Schuman Jorns, M. (2001). "N-methyltryptophan oxidase from Escherichia coli: reaction kinetics with N-methyl amino acid and carbinolamine substrates." Biochemistry 40:1451-1459. Pubmed: 11170473
Koyama, Y., Ohmori, H. (1996). "Nucleotide sequence of the Escherichia coli solA gene encoding a sarcosine oxidase-like protein and characterization of its product." Gene 181:179-183. Pubmed: 8973328
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
Wagner, M. A., Khanna, P., Jorns, M. S. (1999). "Structure of the flavocoenzyme of two homologous amine oxidases: monomeric sarcosine oxidase and N-methyltryptophan oxidase." Biochemistry 38:5588-5595. Pubmed: 10220347