Ribonucleoside-diphosphate reductase 2 subunit alpha (P39452)

Identification
Name:Ribonucleoside-diphosphate reductase 2 subunit alpha
Synonyms:
  • R1E protein
  • Ribonucleotide reductase 2
Gene Name:nrdE
Enzyme Class:
Biological Properties
General Function:Involved in oxidation-reduction process
Specific Function:Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction of the ribonucleotide
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thioredoxin disulfide+1.0Thumb ↔ 1.0Thioredoxin+1.0Thumb
1.0dADP + 1.0Thioredoxin disulfide + 1.0Water ↔ 1.0Thioredoxin + 1.0ADP
ReactionCard
1.0dUDP+1.0Thioredoxin disulfide+1.0Thumb ↔ 1.0Thioredoxin+1.0Thumb
1.0dUDP + 1.0Thioredoxin disulfide + 1.0Water ↔ 1.0Thioredoxin + 1.0Uridine 5'-diphosphate
ReactionCard
1.0dGDP+1.0Thioredoxin disulfide+1.0Thumb ↔ 1.0Thumb+1.0Thioredoxin
1.0dGDP + 1.0Thioredoxin disulfide + 1.0Water ↔ 1.0Guanosine diphosphate + 1.0Thioredoxin
ReactionCard
1.0Thumb+1.0Thioredoxin disulfide+1.0Thumb ↔ 1.0Thioredoxin+1.0Thumb
1.0dCDP + 1.0Thioredoxin disulfide + 1.0Water ↔ 1.0Thioredoxin + 1.0CDP
ReactionCard
1.02'-Deoxyribonucleoside diphosphate+1.0Thioredoxin disulfide+1.0Thumb ↔ 1.0Ribonucleoside diphosphate+1.0Thumb
1.02'-Deoxyribonucleoside diphosphate + 1.0Thioredoxin disulfide + 1.0Water ↔ 1.0Ribonucleoside diphosphate + 1.0Thioredoxin
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0a reduced NrdH glutaredoxin-like protein → 1.0Thumb+1.0an oxidized NrdH glutaredoxin-like protein+1.0dGDP+1.0Thumb
1.0Guanosine diphosphate + 1.0a reduced NrdH glutaredoxin-like protein → 1.0Water + 1.0an oxidized NrdH glutaredoxin-like protein + 1.0dGDP + 1.0dGDP
ReactionCard
1.0Adenosine diphosphate+1.0a reduced NrdH glutaredoxin-like protein+1.0Thumb → 1.0an oxidized NrdH glutaredoxin-like protein+1.0Thumb+1.0dADP+1.0Thumb
1.0Adenosine diphosphate + 1.0a reduced NrdH glutaredoxin-like protein + 1.0ADP → 1.0an oxidized NrdH glutaredoxin-like protein + 1.0Water + 1.0dADP + 1.0dADP
ReactionCard
1.0Thumb+1.0a reduced NrdH glutaredoxin-like protein → 1.0Thumb+1.0Thumb+1.0an oxidized NrdH glutaredoxin-like protein
1.0CDP + 1.0a reduced NrdH glutaredoxin-like protein → 1.0Water + 1.0dCDP + 1.0an oxidized NrdH glutaredoxin-like protein
ReactionCard
Complex Reactions:
1.0glutaredoxin+1.0Thumb → 1.0Thumb+1.0glutaredoxin+1.0Thumb
1.0glutaredoxin + 1.0Uridine 5'-diphosphate → 1.0dUDP + 1.0glutaredoxin + 1.0Water
ReactionCard
1.0Thumb+1.0glutaredoxin → 1.0Thumb+1.0glutaredoxin+1.0Thumb
1.0Guanosine diphosphate + 1.0glutaredoxin → 1.0dGDP + 1.0glutaredoxin + 1.0Water
ReactionCard
1.0Thumb+1.0glutaredoxin → 1.0Thumb+1.0glutaredoxin+1.0Thumb
1.0CDP + 1.0glutaredoxin → 1.0dCDP + 1.0glutaredoxin + 1.0Water
ReactionCard
1.0Thumb+1.0glutaredoxin → 1.0Thumb+1.0glutaredoxin+1.0Thumb
1.0ADP + 1.0glutaredoxin → 1.0dADP + 1.0glutaredoxin + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01341ADPMetaboCard
ECMDB01546CDPMetaboCard
ECMDB21326dADPMetaboCard
ECMDB01245dCDPMetaboCard
ECMDB00960dGDPMetaboCard
ECMDB01000dUDPMetaboCard
ECMDB01201Guanosine diphosphateMetaboCard
ECMDB23749ThioredoxinMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
macromolecular complex
protein complex
ribonucleoside-diphosphate reductase complex
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
nucleoside binding
oxidoreductase activity
oxidoreductase activity, acting on CH or CH2 groups
oxidoreductase activity, acting on CH or CH2 groups, disulfide as acceptor
protein binding
purine nucleoside binding
ribonucleoside-diphosphate reductase activity
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2675
Gene OrientationClockwise
Centisome Percentage:60.34
Left Sequence End2799370
Right Sequence End2801514
Gene Sequence:
>2145 bp
GTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGC
CTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATC
GCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAAC
TCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCC
AGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAA
GACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGCCCAG
TCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAG
GGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGACCCGCAACAGCTTCGGC
GGTGCCAAAAACACCAACATCACCGGCGTTATCGTTAACAAACTGAACGCACCGGTTGAT
GAACAGGGTCGTACTCGCCCGGATCTGTCCGAGATTTTCGACGACTCTTCCAAAGCTAAA
GTAAACAATGTTGATCCGGCGAAGCTGCAAGAATCCAGCCCGCTGCCGGTTCTCGGCGCT
GTGCCGTGGAGCTTTGACCTGATCGCGACTCGTGCGATCGATATGGCTCGCCACCTGAAT
GCGACCATCATCAACGAAGGCGACATCAATACTCGCCGCGTTAAATCCGTCACTTTCTGC
GCACGCAGCATTCCGCACATGCTGGAGCACTTCCGTGCCGGTTCTCTGCTGGTGACTTCC
GCAGACCGTCCTGACGTGCTGGTGGCCGCTTGCCTGGCAGCCATGAACGGCGTAGAAATC
GGTGCCCTGCTGCTGACTGGCGGTTACGAAATGGACGCGCGCATTTCTAAACTGTGCGAA
CGTGCTTTCGCTACCGGCCTGCCGGTATTTATGGTGAACACCAACACCTGGCAGACCTCT
CTGAGCCTGCAGAGCTTCAACCTGGAAGTTCCGGTTGACGATCACGAACGTATCGAGAAA
GTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACT
TCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTATCAGCTGACTGAACTTGCG
CGCAAAGCGGGCAAACGTATCGTACTGCCGGAAGGTGACGAACCGCGTACCGTTAAAGCA
GCCGCTATCTGTGCTGAACGTGGTATCGCAACTTGCGTACTGCTGGGTAATCCGGCAGAG
ATCAACCGTGTTGCAGCGTCTCAGGGTGTAGAACTGGGTGCAGGGATTGAAATCGTTGAT
CCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCGTAAGAACAAAGGC
ATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCTCGGTACGCTGATG
CTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACC
ATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTG
TTCTTCATGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGAT
CCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTC
GGTATCGAACCGCGCGTTGCTATGCTCTCCTACTCCACCGGTACTTCTGGTGCAGGTAGC
GACGTAGAAAAAGTTCGCGAAGCAACTCGTCTGGCGCAGGAAAAACGTCCTGACCTGATG
ATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCG
CCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGT
AACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTG
CAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTC
TACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:714
Protein Molecular Weight:80478
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribonucleoside-diphosphate reductase 2 subunit alpha
MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAFIATHVRPNSVTFSSQQ
QRLNWLVNEGYYDESVLNRYSRDFVITLFTHAHTSGFRFQTFLGAWKFYTSYTLKTFDGK
RYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFL
LRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLED
AFSYANQLGARQGAGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLA
KENAQMALFSPYDVERVYGKPFADVAISQHYDELVADERIRKKYLNARDFFQRLAEIQFE
SGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYTRTGHDISCNLGSL
NIAHTMDSPDFARTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAR
EGIAYGSPEALDFTNLYFYAITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQG
NWQPKTAKVGELFTRSGITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATS
SIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQG
LSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
References
External Links:
ResourceLink
Uniprot ID:P39452
Uniprot Name:RIR3_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675350
Ecogene ID:EG20257
Ecocyc:EG20257
ColiBase:b2675
Kegg Gene:b2675
EchoBASE ID:EB4158
CCDB:RIR3_ECOLI
BacMap:16130589
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jordan, A., Aragall, E., Gibert, I., Barbe, J. (1996). "Promoter identification and expression analysis of Salmonella typhimurium and Escherichia coli nrdEF operons encoding one of two class I ribonucleotide reductases present in both bacteria." Mol Microbiol 19:777-790. Pubmed: 8820648
  • Kubo, K. M., Craig, N. L. (1990). "Bacterial transposon Tn7 utilizes two different classes of target sites." J Bacteriol 172:2774-2778. Pubmed: 2158980
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837