Glutamate/aspartate periplasmic-binding protein (P37902)

Identification
Name:Glutamate/aspartate periplasmic-binding protein
Synonyms:Not Available
Gene Name:gltI
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Part of the binding-protein-dependent transport system for glutamate and aspartate. Binds to both aspartate and glutamate
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0L-Glutamic acid+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0L-Glutamic acid+1.0Thumb
1.0L-Glutamic acid + 1.0Adenosine triphosphate + 1.0Water + 1.0L-Glutamate → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0L-Glutamic acid + 1.0ADP
ReactionCard
1.0L-Aspartic acid+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0L-Aspartic acid+1.0Thumb
1.0L-Aspartic acid + 1.0Adenosine triphosphate + 1.0Water + 1.0L-Aspartic acid → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0L-Aspartic acid + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0L-Glutamate → 1.0ADP + 1.0L-Glutamate + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0L-Aspartic acid → 1.0ADP + 1.0L-Aspartic acid + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00191L-Aspartic acidMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b0655
Gene OrientationCounterclockwise
Centisome Percentage:14.79
Left Sequence End686062
Right Sequence End686970
Gene Sequence:
>909 bp
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCA
GGGGAGCAGTTGTACCGCCATCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATT
GAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGACGGGGCAGCGCGGAACGGTA
GGTATGGGCATTCCTGGCTCAATTTCGCCTTACACCGGTGTGGTGAAGAATGCCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTG
CGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCG
GGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTC
AATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAA
CAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTG
AGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTA
GCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTA
TATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCG
GTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCA
CAAGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:302
Protein Molecular Weight:33420
Protein Theoretical pI:9
Signaling Regions:
  • 1-22
Transmembrane Regions:
  • None
Protein Sequence:
>Glutamate/aspartate periplasmic-binding protein
MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVVGHRESSVPFSYYDNQQ
KVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLLQNGTFDFECGSTTNNVER
QKQAAFSDTIFVVGTRLLTKKGGDIKDFANLKDKAVVVTSGTTSEVLLNKLNEEQKMNMR
IISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKKPDNWEIVGKPQSQEAYGCMLRK
DDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPPKNLNMNFELSDEMKALFKEPNDKA
LN
References
External Links:
ResourceLink
Uniprot ID:P37902
Uniprot Name:GLTI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674535
Ecogene ID:EG12700
Ecocyc:EG12700
ColiBase:b0655
Kegg Gene:b0655
EchoBASE ID:EB2562
CCDB:GLTI_ECOLI
BacMap:16128638
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Fountoulakis, M., Takacs, M. F., Berndt, P., Langen, H., Takacs, B. (1999). "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Electrophoresis 20:2181-2195. Pubmed: 10493123
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Willis, R. C., Furlong, C. E. (1975). "Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli." J Biol Chem 250:2581-2586. Pubmed: 1091636
  • Willis, R. C., Furlong, C. E. (1975). "Purification and properties of a periplasmic glutamate-aspartate binding protein from Escherichia coli K12 strain W3092." J Biol Chem 250:2574-2580. Pubmed: 1091635