2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (P37769)

Identification
Name:2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
Synonyms:
  • 2-keto-3-deoxygluconate 5-dehydrogenase
  • 2-keto-3-deoxygluconate oxidoreductase
  • KDG oxidoreductase
Gene Name:kduD
Enzyme Class:
Biological Properties
General Function:Involved in 2-deoxy-D-gluconate 3-dehydrogenase activity
Specific Function:Catalyzes the reduction of 2,5-diketo-3-deoxygluconate (DKII or 4,6-dihydroxy-2,5-dioxohexanoate) into 2-keto-3- deoxygluconate (KDG or 2-dehydro-3-deoxygluconate) with a concomitant oxidation of NADH
Cellular Location:Not Available
SMPDB Pathways:
  • Collection of Reactions without pathways PW001891
KEGG Pathways:
  • Pentose and glucuronate interconversions ec00040
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Keto-3-deoxy-D-gluconic acid + 1.0NAD ↔ 1.0(4S)-4,6-Dihydroxy-2,5-dioxohexanoate + 1.0NADH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Deoxygluconate + 1.0NAD → 1.03-Dehydro-2-deoxy-D-gluconate + 1.0NADH + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0NAD + 1.02-Deoxygluconate ↔ 1.0Hydrogen ion + 1.0NADH + 1.03-Dehydro-2-deoxy-D-gluconate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Keto-3-deoxy-D-gluconic acid + 1.0NAD → 1.0(4S)-4,6-Dihydroxy-2,5-dioxohexanoate + 1.0NADH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20011(4S)-4,6-Dihydroxy-2,5-dioxohexanoateMetaboCard
ECMDB040502-DeoxygluconateMetaboCard
ECMDB013532-Keto-3-deoxy-D-gluconic acidMetaboCard
ECMDB040473-Dehydro-2-deoxy-D-gluconateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
GO Classification:
Function
2-deoxy-D-gluconate 3-dehydrogenase activity
binding
catalytic activity
NAD or NADH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2842
Gene OrientationCounterclockwise
Centisome Percentage:64.24
Left Sequence End2980519
Right Sequence End2981280
Gene Sequence:
>762 bp
GTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCAT
GTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCC
TTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAAC
GGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAG
GAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTG
CACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAGTCGCTTTAACTCGCGTTTG
CAAGAACGTATTCGTAAATCTGAAGCGCTAACAGCCGGGAATACCGGTCTGACGCTGAAT
ATTGCGGCGAACTACGGTGGACGTTGGGATATAGTCCAGGGAGTCAGGCAACTGGCTGAA
AAGGTGCAGCAAGGAAACCTGCAACCAGATCAGATAGATGAAGAGATGCTAAACCAGCAT
GTCTGTATGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGAGCATCGC
ATTAGTAACTTTTTGCTTTGGCAAATTGCCTATGCCGAACTTTACTTTACAGATGTTCTC
TGGCCCGATTTCGATGAACAAGACTTTGAAGGGGCGTTAAATGCCTTTGCTAATCGAGAG
CGTCGTTTCGGCGGCACCGAGCCCGGTGATGAAACAGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:253
Protein Molecular Weight:27070
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVEPTETIEQVTALGRRFL
SLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDALEFSEKDWDDVMNLNIKSV
FFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTASKSGVMGVTRLMANEWAKHN
INVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRWGLPSDLMGPIVFLASSASDYVN
GYTIAVDGGWLAR
References
External Links:
ResourceLink
Uniprot ID:P37769
Uniprot Name:KDUD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674367
Ecogene ID:EG12361
Ecocyc:EG12361
ColiBase:b2842
Kegg Gene:b2842
EchoBASE ID:EB2264
CCDB:KDUD_ECOLI
BacMap:16130746
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Condemine, G., Robert-Baudouy, J. (1991). "Analysis of an Erwinia chrysanthemi gene cluster involved in pectin degradation." Mol Microbiol 5:2191-2202. Pubmed: 1766386
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Maiden, M. C., Jones-Mortimer, M. C., Henderson, P. J. (1988). "The cloning, DNA sequence, and overexpression of the gene araE coding for arabinose-proton symport in Escherichia coli K12." J Biol Chem 263:8003-8010. Pubmed: 2836407