dTDP-4-dehydrorhamnose reductase (P37760)

Identification
Name:dTDP-4-dehydrorhamnose reductase
Synonyms:
  • dTDP-4-keto-L-rhamnose reductase
  • dTDP-6-deoxy-L-lyxo-4-hexulose reductase
  • dTDP-6-deoxy-L-mannose dehydrogenase
  • dTDP-L-rhamnose synthase
Gene Name:rfbD
Enzyme Class:
Biological Properties
General Function:Involved in dTDP-4-dehydrorhamnose reductase activity
Specific Function:Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0dTDP-4-Dehydro-6-deoxy-L-mannose + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0Deoxythymidine diphosphate-L-rhamnose + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Deoxythymidine diphosphate-L-rhamnose + 1.0NADP ↔ 1.0dTDP-4-Dehydro-6-deoxy-L-mannose + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0dTDP-4-dehydro-beta-L-rhamnose+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0dTDP-4-dehydro-beta-L-rhamnose + 1.0NADPH + 1.0Hydrogen ion → 1.0NADP + 1.0Deoxythymidine diphosphate-L-rhamnose
ReactionCard
1.0dTDP-4-dehydro-beta-L-rhamnose+1.0Thumb+1.0Thumb → 1.0dTDP-6-deoxy-L-mannose+1.0Thumb
1.0dTDP-4-dehydro-beta-L-rhamnose + 1.0NADPH + 1.0Hydrogen ion → 1.0dTDP-6-deoxy-L-mannose + 1.0NADP
ReactionCard
1.0dTDP-4-dehydro-beta-L-rhamnose+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0dTDP-4-dehydro-beta-L-rhamnose + 1.0NADH + 1.0Hydrogen ion → 1.0NADP + 1.0TDP-Rhamnose
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0dTDP-4-dehydro-6-deoxy-β-L-mannose
1.0NADP + 1.0Deoxythymidine diphosphate-L-rhamnose ↔ 1.0Hydrogen ion + 1.0NADPH + 1.0dTDP-4-dehydro-6-deoxy-β-L-mannose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB06354Deoxythymidine diphosphate-L-rhamnoseMetaboCard
ECMDB21327dTDP-4-Dehydro-6-deoxy-L-mannoseMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB20537TDP-RhamnoseMetaboCard
GO Classification:
Function
binding
catalytic activity
dTDP-4-dehydrorhamnose reductase activity
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process
carbohydrate metabolic process
cellular polysaccharide biosynthetic process
extracellular polysaccharide biosynthetic process
metabolic process
polysaccharide biosynthetic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b2040
Gene OrientationCounterclockwise
Centisome Percentage:45.46
Left Sequence End2109101
Right Sequence End2110000
Gene Sequence:
>900 bp
ATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGC
TTCACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGT
CGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCA
ACTGAAGCGGGCGAGCATCTTTTGTCTGTTCTTGGCCCTATGTTGCATGACATAGATTCA
GCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACT
GTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCAT
CCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGT
TTTGATGCAGGCGTCCGTCTGGGTGGGGAGATGGATAAAGATATGATCGCCATTCGAATC
GGGCCAGATATACCAATGGCTATTGTTGGCTCACCGGATTATTTTTCTCGCCGAAGTGTT
CCAACGTCAGTGTCACAATTAATAGATCATCAGGCAATTAATTTGTATCTTCCCACATCG
GGTACAGCAAATCGCTGGAGATTAATACGCGGTGGACGTGAAGTTCGTGTTCGCATGGAA
GGTCAGCTTTTACTGAATACGATAGACCTGATCATTGATGCTGCAATTGATGGGCATGGA
TTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAACTGATACGT
GTTTTGGATAAATTCACACCAGATTTACCCGGTTATCACCTGTACTATCCACACCGTCGA
CATGCTGGCTCGGCATTCTCATTATTTATAGATAGGCTGAAGTATAAAGGTGCTGTTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:299
Protein Molecular Weight:32694
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>dTDP-4-dehydrorhamnose reductase
MNILLFGKTGQVGWELQRALAPLGNLIAFDVHSTDYCGDFSNPEGVAETVRSIRPDIIVN
AAAHTAVDKAESEPEFAQLINATSVEAIAKAANEVGAWVIHYSTDYVFPGNGDMPWLETD
ATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGNNFAKTMLRLAKEREELAVIN
DQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVASGTTTWYDYAALVFEEARKAGIP
LALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFALVLPDWQVGVKRMLNELFTTTAI
References
External Links:
ResourceLink
Uniprot ID:P37760
Uniprot Name:RMLD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742185
Ecogene ID:EG12411
Ecocyc:EG12411
ColiBase:b2040
Kegg Gene:b2040
EchoBASE ID:EB2310
CCDB:RMLD_ECOLI
BacMap:16129980
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Stevenson, G., Neal, B., Liu, D., Hobbs, M., Packer, N. H., Batley, M., Redmond, J. W., Lindquist, L., Reeves, P. (1994). "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster." J Bacteriol 176:4144-4156. Pubmed: 7517391