Nitrite extrusion protein 2 (P37758)

Identification
Name:Nitrite extrusion protein 2
Synonyms:
  • Nitrite facilitator 2
Gene Name:narU
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transmembrane transport
Specific Function:Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
SMPDB Reactions:
1.0Nitrate+1.0Thumb → 1.0Nitrate
1.0Nitrate + 1.0Nitrate → 1.0Nitrate
ReactionCard
1.0Nitrite+1.0Thumb → 1.0Nitrite
1.0Nitrite + 1.0Nitrite → 1.0Nitrite
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB02878NitrateMetaboCard
ECMDB02786NitriteMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
anion transmembrane transporter activity
inorganic anion transmembrane transporter activity
ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
transmembrane transport
transport
Gene Properties
Blattner:b1469
Gene OrientationCounterclockwise
Centisome Percentage:33.21
Left Sequence End1540696
Right Sequence End1542084
Gene Sequence:
>1389 bp
ATGGCACTGCAAAATGAGAAAAATAGTCGTTATCTTTTGCGCGACTGGAAACCAGAAAAT
CCGGCCTTCTGGGAAAATAAAGGAAAACATATTGCTCGAAGAAATCTCTGGATATCAGTC
AGTTGTCTACTTCTTGCCTTCTGTGTCTGGATGCTATTTAGCGCAGTTACCGTTAATCTC
AATAAAATCGGTTTTAATTTTACTACCGATCAACTCTTTTTATTAACCGCATTACCCTCC
GTTTCTGGCGCATTATTGCGTGTTCCCTACTCCTTTATGGTGCCTATATTCGGTGGACGC
CGATGGACGGTTTTTAGTACTGCAATCCTGATTATTCCTTGCGTCTGGCTCGGAATTGCC
GTGCAAAATCCGAATACTCCTTTTGGGATATTTATCGTTATCGCTTTGCTATGCGGTTTT
GCAGGTGCAAACTTTGCTTCGAGCATGGGCAATATCAGTTTCTTCTTTCCAAAAGCCAAA
CAAGGGAGCGCTCTTGGGATTAATGGCGGATTAGGAAACTTAGGTGTAAGTGTAATGCAG
CTGGTTGCACCGCTGGTCATTTTTGTACCTGTATTTGCCTTTCTCGGCGTCAATGGCGTA
CCGCAGGCCGACGGTTCGGTGATGTCGCTGGCGAATGCCGCATGGATTTGGGTACCGCTA
CTGGCGATTGCCACGATCGCCGCATGGTCAGGGATGAATGATATCGCCAGTTCACGCGCC
TCAATTGCCGACCAGCTCCCTGTCTTACAACGCCTGCATCTCTGGCTGCTGAGCCTGCTT
TACCTTGCCACCTTCGGTTCGTTTATCGGTTTTTCTGCGGGTTTTGCCATGCTGGCAAAA
ACCCAGTTCCCGGATGTGAATATTCTGCGCCTGGCGTTCTTTGGCCCATTTATCGGTGCC
ATCGCGCGGTCGGTTGGTGGTGCTATTTCCGATAAGTTCGGCGGCGTGCGGGTGACGTTG
ATCAACTTTATTTTTATGGCGATTTTCAGTGCCCTGCTGTTCCTTACCTTACCGGGCACA
GGCTCCGGTAATTTCATCGCCTTTTACGCCGTATTTATGGGGCTGTTTCTGACTGCGGGT
CTGGGAAGTGGTTCTACTTTCCAGATGATCGCCGTCATCTTTCGCCAGATAACCATTTAT
CGGGTAAAGATGAAAGGCGGTAGTGATGAGCAAGCTCATAAAGAAGCCGTCACCGAAACG
GCGGCGGCTCTGGGCTTTATCTCAGCCATTGGCGCAGTGGGCGGCTTTTTTATTCCGCAG
GCGTTTGGCATGTCGCTCAATATGACCGGCTCTCCGGTCGGCGCGATGAAAGTGTTTTTA
ATCTTCTACATCGTTTGTGTGCTGCTGACCTGGCTGGTTTATGGTCGGCGGAAGTTCAGC
CAAAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:462
Protein Molecular Weight:49890
Protein Theoretical pI:11
Signaling Regions:
  • None
Transmembrane Regions:
  • 36-56
  • 77-97
  • 102-122
  • 126-146
  • 181-201
  • 207-227
  • 259-279
  • 288-308
  • 318-338
  • 345-365
  • 402-422
  • 433-453
Protein Sequence:
>Nitrite extrusion protein 2
MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLLLAFCVWMLFSAVTVNL
NKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIA
VQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQ
LVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRA
SIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGA
IARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAG
LGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAHKEAVTETAAALGFISAIGAVGGFFIPQ
AFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK
References
External Links:
ResourceLink
Uniprot ID:P37758
Uniprot Name:NARU_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742401
Ecogene ID:EG12153
Ecocyc:EG12153
ColiBase:b1469
Kegg Gene:b1469
EchoBASE ID:EB2073
CCDB:NARU_ECOLI
BacMap:16129428
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blasco, F., Iobbi, C., Ratouchniak, J., Bonnefoy, V., Chippaux, M. (1990). "Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon." Mol Gen Genet 222:104-111. Pubmed: 2233673
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bonnefoy, V., Demoss, J. A. (1994). "Nitrate reductases in Escherichia coli." Antonie Van Leeuwenhoek 66:47-56. Pubmed: 7747940
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553