Glucose-1-phosphate thymidylyltransferase 1 (P37744)

Identification
Name:Glucose-1-phosphate thymidylyltransferase 1
Synonyms:
  • G1P-TT 1
  • dTDP-glucose pyrophosphorylase 1
  • dTDP-glucose synthase 1
Gene Name:rmlA1
Enzyme Class:
Biological Properties
General Function:Involved in glucose-1-phosphate thymidylyltransferase activity
Specific Function:Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis
Cellular Location:Not Available
SMPDB Pathways:
  • O-antigen building blocks biosynthesis PW002089
  • Secondary Metabolites: enterobacterial common antigen biosynthesis PW000959
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Thymidine 5'-triphosphate + 1.0Glucose 1-phosphate + 1.0Hydrogen ion ↔ 1.0dTDP-D-Glucose + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Thymidine 5'-triphosphate + 1.0Glucose 1-phosphate ↔ 1.0Pyrophosphate + 1.0dTDP-D-Glucose
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Thymidine 5'-triphosphate + 1.0Hydrogen ion + 1.0Glucose 1-phosphate → 1.0Pyrophosphate + 1.0dTDP-D-Glucose
ReactionCard
1.0α-D-glucose 1-phosphate+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0α-D-glucose 1-phosphate + 1.0Thymidine 5'-triphosphate + 1.0Hydrogen ion → 1.0dTDP-D-Glucose + 1.0Pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Thymidine 5'-triphosphate + 1.0Glucose 1-phosphate + 1.0Hydrogen ion ↔ 1.0dTDP-D-Glucose + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01328dTDP-D-GlucoseMetaboCard
ECMDB01586Glucose 1-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB20298TDP-GlucoseMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
GO Classification:
Function
catalytic activity
glucose-1-phosphate thymidylyltransferase activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
carbohydrate metabolic process
cellular polysaccharide biosynthetic process
extracellular polysaccharide biosynthetic process
metabolic process
polysaccharide biosynthetic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b2039
Gene OrientationCounterclockwise
Centisome Percentage:45.44
Left Sequence End2108162
Right Sequence End2109043
Gene Sequence:
>882 bp
ATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTG
AATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGAC
GAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGTGCTACTGGCTGGGCGAACTTCAGC
CGCAATATCGATCCGCTGGTAGAGGCGGGCTATCGGGTGATCCTGCTGGATTGTCCGGGT
TGGGGCAAGAGCGATTCGGTCGTTAATAGTGGTTCGCGATCGGATCTTAATGCACGAATC
CTGAAAAGCGTGGTGGATCAACTGGATATCGCCAAAATCCACCTGCTGGGCAACTCGATG
GGGGGCCATAGTTCTGTGGCGTTCACCCTTAAATGGCCGGAGCGCGTCGGCAAACTGGTG
CTGATGGGCGGCGGTACGGGCGGCATGAGTTTGTTTACGCCGATGCCAACCGAAGGTATT
AAGCGACTGAATCAGCTTTATCGTCAGCCGACTATCGAAAACCTGAAGCTGATGATGGAT
ATCTTCGTTTTTGATACCAGCGATTTGACCGACGCCCTGTTTGAAGCGCGCCTGAATAAT
ATGCTGTCGCGCCGCGATCACCTGGAAAACTTCGTTAAGAGCCTGGAAGCTAATCCGAAA
CAGTTCCCGGATTTTGGCCCACGTCTGGCGGAAATCAAAGCGCAAACCCTGATTGTCTGG
GGGCGCAACGACCGCTTTGTGCCGATGGATGCGGGTCTGCGTCTGCTGTCCGGCATTGCC
GGTTCTGAACTGCATATCTTCCGCGACTGTGGTCACTGGGCGCAGTGGGAACATGCCGAC
GCTTTCAATCAACTGGTGCTGAATTTCCTCGCACGCCCTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:293
Protein Molecular Weight:32693
Protein Theoretical pI:5
PDB File:1H5S
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glucose-1-phosphate thymidylyltransferase 1
MKMRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTLMLAGIRDILIISTPQD
TPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGGDDCALVLGDNIFYGHDLP
KLMEAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISLEEKPLEPKSNYAVTGLYFYD
NDVVQMAKNLKPSARGELEITDINRIYLEQGRLSVAMMGRGYAWLDTGTHQSLIEASNFI
ATIEERQGLKVSCPEEIAFRKGFIDVEQVRKLAVPLIKNNYGQYLYKMTKDSN
References
External Links:
ResourceLink
Uniprot ID:P37744
Uniprot Name:RMLA1_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674491
PDB ID:1H5S
Ecogene ID:EG11978
Ecocyc:EG11978
ColiBase:b2039
Kegg Gene:b2039
EchoBASE ID:EB1921
CCDB:RMLA1_ECOLI
BacMap:16129979
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Stevenson, G., Neal, B., Liu, D., Hobbs, M., Packer, N. H., Batley, M., Redmond, J. W., Lindquist, L., Reeves, P. (1994). "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster." J Bacteriol 176:4144-4156. Pubmed: 7517391
  • Yao, Z., Valvano, M. A. (1994). "Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a." J Bacteriol 176:4133-4143. Pubmed: 7517390
  • Zuccotti, S., Zanardi, D., Rosano, C., Sturla, L., Tonetti, M., Bolognesi, M. (2001). "Kinetic and crystallographic analyses support a sequential-ordered bi bi catalytic mechanism for Escherichia coli glucose-1-phosphate thymidylyltransferase." J Mol Biol 313:831-843. Pubmed: 11697907