ADP-heptose--LPS heptosyltransferase 2 (P37692)

Identification
Name:ADP-heptose--LPS heptosyltransferase 2
Synonyms:
  • ADP-heptose--LPS heptosyltransferase II
Gene Name:rfaF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transferase activity, transferring glycosyl groups
Specific Function:Specific function unknown
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
SMPDB Reactions:
1.0heptosyl-Kdo2-lipid A+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0heptosyl-Kdo2-lipid A + 1.0ADP-L-glycero-beta-D-manno-heptose + 1.0Heptosyl-KDO2-lipid A → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0(heptosyl)2-Kdo2-lipid A + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Heptosyl-KDO2-lipid A + 1.0ADP-L-Glycero-D-manno-heptose → 1.0Hydrogen ion + 1.0Heptosyl2-KDO2-lipid A + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0heptosyl-kdo2-lipidA → 1.0Thumb+1.0Thumb+1.0heptosyl-heptosyl-kdo2-lipidA
1.0ADP-L-Glycero-D-manno-heptose + 1.0heptosyl-kdo2-lipidA → 1.0ADP + 1.0Hydrogen ion + 1.0heptosyl-heptosyl-kdo2-lipidA
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24219(heptosyl)2-Kdo2-lipid AMetaboCard
ECMDB01341ADPMetaboCard
ECMDB24180ADP-L-glycero-beta-D-manno-heptoseMetaboCard
ECMDB20113ADP-L-Glycero-D-manno-heptoseMetaboCard
ECMDB20526Heptosyl-KDO2-lipid AMetaboCard
ECMDB20527Heptosyl2-KDO2-lipid AMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
Process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3620
Gene OrientationClockwise
Centisome Percentage:81.75
Left Sequence End3792952
Right Sequence End3793998
Gene Sequence:
>1047 bp
ATGAACAGAACTGACGAACTCCGTACTGCGCGTATTGAGAGCCTGGTAACGCCCGCCGAA
CTCGCGCTACGGTATCCCGTAACGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGC
AGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGC
TCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAAC
CAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTT
GTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCAC
GGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCG
ACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCG
ATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGT
CCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGC
GCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTAT
CAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTAT
CATGCCGATGATATCGCCGCAGCCTGCGATACGCTGCACGAGTTTGATTTACCTGAACAT
CTGGTGGTGGATTTCAGCCACGGTAACTGCCAGAAGCAGCACCGTCGCCAGTTAGAAGTT
TGTGAGGATATTTGTCAGCAAATCCGCAATGGCTCTACGGCGATTGCTGGAATTATGGCG
GAAAGTTTCCTGCGCGAAGGAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGT
CAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTC
GCCTCTGCGGTAGATACCCGCTTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:348
Protein Molecular Weight:39042
Protein Theoretical pI:8
PDB File:1PSW
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>ADP-heptose--LPS heptosyltransferase 2
MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG
HGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGIPHRTGWRGEMRYGLLNDV
RVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQLQVSEGEKSYTCNQFSLSSER
PMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVLFGSAKDHEAGNEILAALNTEQQ
AWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVAAALNRPLVALYGPSSPDFTPPLSH
KARVIRLITGYHKVRKGDAAEGYHQSLIDITPQRVLEELNALLLQEEA
References
External Links:
ResourceLink
Uniprot ID:P37692
Uniprot Name:RFAF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742785
PDB ID:1PSW
Ecogene ID:EG12210
Ecocyc:EG12210
ColiBase:b3620
Kegg Gene:b3620
EchoBASE ID:EB2124
CCDB:RFAF_ECOLI
BacMap:16131491
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chen, L., Coleman, W. G. Jr (1993). "Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis." J Bacteriol 175:2534-2540. Pubmed: 8478319
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pegues, J. C., Chen, L. S., Gordon, A. W., Ding, L., Coleman, W. G. Jr (1990). "Cloning, expression, and characterization of the Escherichia coli K-12 rfaD gene." J Bacteriol 172:4652-4660. Pubmed: 2198271
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620