2,3-diketo-L-gulonate reductase (P37672)

Identification
Name:2,3-diketo-L-gulonate reductase
Synonyms:
  • 2,3-DKG reductase
  • 3-dehydro-L-gulonate 2-dehydrogenase
Gene Name:dlgD
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Specific Function:Catalyzes the reduction of 2,3-diketo-L-gulonate in the presence of NADH, to form 3-keto-L-gulonate
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:
KEGG Pathways:
  • Ascorbate and aldarate metabolism ec00053
  • Pentose and glucuronate interconversions ec00040
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02,3-Diketo-L-gulonate + 1.0Hydrogen ion + 1.0NADH → 1.03-Dehydro-L-gulonate + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Dehydro-L-gulonate + 1.0NAD ↔ 1.02,3-Diketo-L-gulonate + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Dehydro-L-gulonate + 1.0NADP ↔ 1.02,3-Diketo-L-gulonate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.03-Dehydro-L-gulonate + 1.0NAD + 1.0NADP ↔ 1.02,3-Diketo-L-gulonate + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.02,3-Diketo-L-gulonate+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02,3-Diketo-L-gulonate + 1.0NADH + 1.0Hydrogen ion + 1.02,3-Diketo-L-gulonate → 1.03-Dehydro-L-gulonate + 1.0NAD
ReactionCard
1.02-Keto-L-gluconate+1.0NADPH+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Keto-L-gluconate + 1.0NADPH + 1.0Hydrogen ion + 1.02-Dehydro-D-gluconate + 1.0NADPH → 1.0Gluconic acid + 1.0NADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02,3-Diketo-L-gulonate + 1.0Hydrogen ion + 1.0NADH → 1.03-Dehydro-L-gulonate + 1.0NAD
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB065112,3-Diketo-L-gulonateMetaboCard
ECMDB040422-Dehydro-D-gluconateMetaboCard
ECMDB063343-Dehydro-L-gulonateMetaboCard
ECMDB214943-Keto-L-gulonic acidMetaboCard
ECMDB00625Gluconic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Function
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b3575
Gene OrientationClockwise
Centisome Percentage:80.63
Left Sequence End3740756
Right Sequence End3741754
Gene Sequence:
>999 bp
ATGAAACTTTTTCGTATCCTCGATCCTTTCACCTTAACCCTGATCACGGTGGTGTTGCTG
GCCTCTTTCTTTCCGGCCAGAGGCGATTTCGTCCCCTTCTTTGAAAATCTGACCACCGCA
GCTATTGCCCTGCTGTTCTTTATGCACGGCGCGAAGTTGTCGCGTGAGGCGATTATTGCT
GGCGGTGGTCACTGGCGACTGCATTTGTGGGTAATGTGCAGCACCTTCGTGCTGTTTCCG
ATTCTGGGTGTACTGTTTGCCTGGTGGAAACCGGTAAATGTCGACCCGATGCTCTACTCC
GGTTTTCTCTACTTGTGCATTCTCCCGGCTACCGTGCAGTCTGCAATCGCCTTCACGTCA
ATGGCGGGCGGTAACGTCGCGGCAGCGGTTTGTTCTGCGTCGGCATCCAGCCTGCTGGGG
ATTTTCCTTTCACCATTGCTGGTTGGTCTGGTGATGAATGTTCACGGTGCAGGGGGCAGC
CTTGAGCAGGTCGGTAAAATTATGCTGCAACTGCTGCTGCCGTTTGTGTTGGGGCATCTT
TCCCGGCCGTGGATTGGTGACTGGGTGTCGCGCAATAAAAAATGGATTGCGAAAACTGAC
CAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATC
TGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGTTCTTCTG
GCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGAT
GAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCA
AACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCAT
CAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAG
TTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:332
Protein Molecular Weight:36572
Protein Theoretical pI:5
PDB File:1S20
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2,3-diketo-L-gulonate reductase
MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSHGVNRFPRFIQQLENGD
IIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAADHGIGLVALRNANHWMRG
GSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLIVAIPSTPITMVDMSMSMFSY
GMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKGSGMSIVLDMIATLL
SDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRDAKLQRIMDYVTSAERADENQAI
RLPGHEFTTLLAENRRNGITVDDSVWAKIQAL
References
External Links:
ResourceLink
Uniprot ID:P37672
Uniprot Name:DLGD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675394
PDB ID:1S20
Ecogene ID:EG12279
Ecocyc:EG12279
ColiBase:b3575
Kegg Gene:b3575
EchoBASE ID:EB2187
CCDB:DLGD_ECOLI
BacMap:16131446
General Reference:
  • Badia, J., Ibanez, E., Sabate, M., Baldoma, L., Aguilar, J. (1998). "A rare 920-kilobase chromosomal inversion mediated by IS1 transposition causes constitutive expression of the yiaK-S operon for carbohydrate utilization in Escherichia coli." J Biol Chem 273:8376-8381. Pubmed: 9525947
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Forouhar, F., Lee, I., Benach, J., Kulkarni, K., Xiao, R., Acton, T. B., Montelione, G. T., Tong, L. (2004). "A novel NAD-binding protein revealed by the crystal structure of 2,3-diketo-L-gulonate reductase (YiaK)." J Biol Chem 279:13148-13155. Pubmed: 14718529
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Yew, W. S., Gerlt, J. A. (2002). "Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of the yjf-sga and yia-sgb operons." J Bacteriol 184:302-306. Pubmed: 11741871