Cobalamin synthase (P36561)

Identification
Name:Cobalamin synthase
Synonyms:Not Available
Gene Name:cobS
Enzyme Class:
Biological Properties
General Function:Involved in cobalamin 5'-phosphate synthase activity
Specific Function:Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
  • adenosylcobalamin salvage from cobinamide PW001884
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosylcobinamide-GDP + 1.0N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole → 1.0Adenosylcobalamin + 1.0Guanosine monophosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosylcobalamin + 1.0Guanosine monophosphate ↔ 1.0Adenosylcobinamide-GDP + 1.0N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosylcobinamide-GDP + 1.0N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole + 1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole ↔ 1.0Guanosine monophosphate + 1.0Adenosylcobalamin + 1.0Adenosylcobalamin 5'-phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Adenosylcobinamide-GDP+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole + 1.0Adenosylcobinamide-GDP + 1.0Adenosylcobinamide-GDP → 1.0Hydrogen ion + 1.0GMP + 1.0Adenosylcobalamin 5'-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosylcobinamide-GDP + 1.0N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole → 1.0Adenosylcobalamin + 1.0Guanosine monophosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0adenosylcobalamin 5'-phosphate+1.0Thumb+1.0Thumb
1.0Adenosylcobinamide-GDP + 1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole → 1.0adenosylcobalamin 5'-phosphate + 1.0Guanosine monophosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ? 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosylcobinamide-GDP + 1.0N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole ? 1.0Hydrogen ion + 1.0Adenosylcobalamin + 1.0Guanosine monophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB02086AdenosylcobalaminMetaboCard
ECMDB23095Adenosylcobalamin 5'-phosphateMetaboCard
ECMDB12185Adenosylcobinamide-GDPMetaboCard
ECMDB04040Cob(I)alaminMetaboCard
ECMDB23235GMPMetaboCard
ECMDB01397Guanosine monophosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB03882N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazoleMetaboCard
ECMDB11112N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazoleMetaboCard
GO Classification:
Function
catalytic activity
cobalamin 5'-phosphate synthase activity
phosphotransferase activity, for other substituted phosphate groups
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cobalamin biosynthetic process
metabolic process
nitrogen compound metabolic process
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process
Gene Properties
Blattner:b1992
Gene OrientationCounterclockwise
Centisome Percentage:44.45
Left Sequence End2062503
Right Sequence End2063246
Gene Sequence:
>744 bp
ATGAAAAACACCGAACTGGAACAACTGATTAACGAAAAACTGAACAGCGCGGCGATTAGT
GATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACC
GGTGTCACCGCCAGCCAGGCTTTGCTCGATGAGGCAGTGCGTCTGGGCGCGGATGCGGTC
ATTGTGCATCATGGCTACTTCTGGAAAGGAGAGTCTCCGGTCATTCGCGGAATGAAGCGT
AACCGTTTAAAAACGTTGCTGGCGAATGATATCAACCTGTATGGCTGGCATTTGCCGCTT
GACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTC
ATGGGCGAAATTGAGCCGTTGGTGCCGTGGGGCGAACTGACCATGCCTGTGCCGGGACTG
GAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACC
GGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGAT
AGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATT
CATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGT
GGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTT
ATTGATATTCCTAATCCTGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:247
Protein Molecular Weight:26385
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 34-54
  • 59-79
  • 113-133
  • 138-158
  • 194-214
Protein Sequence:
>Cobalamin synthase
MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLLGAISGLVFMVLQAWCG
APLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFV
VLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGRQ
TCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGEL
VFLLALL
References
External Links:
ResourceLink
Uniprot ID:P36561
Uniprot Name:COBS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062306
Ecogene ID:EG12150
Ecocyc:EG12150
ColiBase:b1992
Kegg Gene:b1992
EchoBASE ID:EB2070
CCDB:COBS_ECOLI
BacMap:16129933
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Lawrence, J. G., Roth, J. R. (1995). "The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli." J Bacteriol 177:6371-6380. Pubmed: 7592411