Cytochrome c biogenesis ATP-binding export protein CcmA (P33931)

Identification
Name:Cytochrome c biogenesis ATP-binding export protein CcmA
Synonyms:
  • Heme exporter protein A
Gene Name:ccmA
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex CcmAB involved in the biogenesis of c-type cytochromes; once thought to export heme, this seems not to be the case, but its exact role is uncertain. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Heme → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Heme
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0heme(In) → 1.0Thumb+1.0Thumb+1.0heme(Out)
1.0Adenosine triphosphate + 1.0Water + 1.0heme(In) → 1.0ADP + 1.0Inorganic phosphate + 1.0heme(Out)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB03178HemeMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
transporter activity
Process
cellular component assembly
cellular component organization
cellular component organization or biogenesis
cellular protein complex assembly
cytochrome complex assembly
macromolecular complex assembly
protein complex assembly
Gene Properties
Blattner:b2201
Gene OrientationCounterclockwise
Centisome Percentage:49.47
Left Sequence End2295043
Right Sequence End2295666
Gene Sequence:
>624 bp
ATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTG
GTGGCGTATGACTTATCTGCCCACATGCTGGATGTCGTGGCACAAGCTGCCGAAGCCCGG
CAACTGAAAAATATCACCACCCGCCAGGGATATGCCGAAAGTCTGCCATTTGCCGATAAC
GCATTTGATATTGTTATCAGCCGTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCA
CTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATG
TCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACC
TCTCACGTACGAAACTACGCCAGCGGTGAGTGGTTGACGTTAATCAATGAAGCCAATCTG
ATAGTTGATAATTTAATTACAGATAAGTTACCGCTGGAATTTTCTTCATGGGTCGCGAGA
ATGCGTACGCCAGAAGCGTTAGTAGACGCTATTCGCATTTACCAACAGAGCGCATCGACA
GAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATG
GTAGATGCACATAAAGCGGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:207
Protein Molecular Weight:23053
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Cytochrome c biogenesis ATP-binding export protein CcmA
MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLLRLLTGLSRPDAGEV
LWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDGDTAQCLEALAQAGLAG
FEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVNGVDRLTQRMAQHTEQGGI
VILTTHQPLNVAESKIRRISLTQTRAA
References
External Links:
ResourceLink
Uniprot ID:P33931
Uniprot Name:CCMA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902947
Ecogene ID:EG12059
Ecocyc:EG12059
ColiBase:b2201
Kegg Gene:b2201
EchoBASE ID:EB1989
CCDB:CCMA_ECOLI
BacMap:90111402
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cook, G. M., Poole, R. K. (2000). "Oxidase and periplasmic cytochrome assembly in Escherichia coli K-12: CydDC and CcmAB are not required for haem-membrane association." Microbiology 146 ( Pt 2):527-536. Pubmed: 10708391
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Schulz, H., Fabianek, R. A., Pellicioli, E. C., Hennecke, H., Thony-Meyer, L. (1999). "Heme transfer to the heme chaperone CcmE during cytochrome c maturation requires the CcmC protein, which may function independently of the ABC-transporter CcmAB." Proc Natl Acad Sci U S A 96:6462-6467. Pubmed: 10339610
  • Thony-Meyer, L., Fischer, F., Kunzler, P., Ritz, D., Hennecke, H. (1995). "Escherichia coli genes required for cytochrome c maturation." J Bacteriol 177:4321-4326. Pubmed: 7635817