Nickel import ATP-binding protein NikD (P33593)

Identification
Name:Nickel import ATP-binding protein NikD
Synonyms:Not Available
Gene Name:nikD
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex NikABCDE involved in nickel import. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Nickel → 1.0ADP + 1.0Hydrogen ion + 1.0Nickel + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Ni(2+)(Out) → 1.0Thumb+1.0Thumb+1.0Ni(2+)(In)
1.0Adenosine triphosphate + 1.0Water + 1.0Ni(2+)(Out) → 1.0ADP + 1.0Inorganic phosphate + 1.0Ni(2+)(In)
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Nickel → 1.0ADP + 1.0Inorganic phosphate + 1.0Nickel
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB21391NickelMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
plasma membrane
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
binding
catalytic activity
cation binding
cation transmembrane transporter activity
di-, tri-valent inorganic cation transmembrane transporter activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
inorganic cation transmembrane transporter activity
ion binding
ion transmembrane transporter activity
metal ion binding
nickel ion binding
nickel ion transmembrane transporter activity
nickel-transporting ATPase activity
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
substrate-specific transmembrane transporter activity
transition metal ion binding
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
metal ion transport
nickel ion transport
transition metal ion transport
transport
Gene Properties
Blattner:b3479
Gene OrientationClockwise
Centisome Percentage:77.92
Left Sequence End3615038
Right Sequence End3615802
Gene Sequence:
>765 bp
ATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTG
GTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTG
CTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGG
CTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGT
TTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAA
ACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGC
CCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTT
ATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATT
CTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTG
CTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGC
AGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTG
ACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTG
ATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:254
Protein Molecular Weight:26820
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Nickel import ATP-binding protein NikD
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQT
AGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTA
AIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESPFIIADEPTTDLDVVAQARIL
DLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS
References
External Links:
ResourceLink
Uniprot ID:P33593
Uniprot Name:NIKD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674310
Ecogene ID:EG12078
Ecocyc:EG12078
ColiBase:b3479
Kegg Gene:b3479
EchoBASE ID:EB2003
CCDB:NIKD_ECOLI
BacMap:16131351
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Navarro, C., Wu, L. F., Mandrand-Berthelot, M. A. (1993). "The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel." Mol Microbiol 9:1181-1191. Pubmed: 7934931
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620