GDP-mannose mannosyl hydrolase (P32056)

Identification
Name:GDP-mannose mannosyl hydrolase
Synonyms:
  • GDPMH
  • Colanic acid biosynthesis protein wcaH
Gene Name:nudD
Enzyme Class:Not Available
Biological Properties
General Function:Involved in hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
Specific Function:Could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. May be involved in the degradation of GDP-mannose and GDP-glucose, diverting the GDP to the synthesis of GDP-fucose as required. Might also be involved in the biosynthesis of the slime polysaccharide colanic acid
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Guanosine diphosphate mannose + 1.0Water → 1.0Guanosine diphosphate + 1.0Hydrogen ion + 1.0D-Mannose
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanosine diphosphate mannose + 1.0Water → 1.0Guanosine diphosphate + 1.0D-Mannose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00169D-MannoseMetaboCard
ECMDB01201Guanosine diphosphateMetaboCard
ECMDB01163Guanosine diphosphate mannoseMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
Gene Properties
Blattner:b2051
Gene OrientationCounterclockwise
Centisome Percentage:45.77
Left Sequence End2123767
Right Sequence End2124246
Gene Sequence:
>480 bp
ATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATG
CCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTG
ATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATT
ATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAA
GCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTAT
GAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAA
GGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTC
CACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:159
Protein Molecular Weight:18273
Protein Theoretical pI:5
PDB File:1RYA
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>GDP-mannose mannosyl hydrolase
MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGYWFVPGGRVQKDETLEA
AFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHYVVLGFRFRVSEEELLLPD
EQHDDYRWLTSDALLASDNVHANSRAYFLAEKRTGVPGL
References
External Links:
ResourceLink
Uniprot ID:P32056
Uniprot Name:NUDD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321929
PDB ID:1RYA
Ecogene ID:EG11789
Ecocyc:EG11789
ColiBase:b2051
Kegg Gene:b2051
EchoBASE ID:EB1737
CCDB:NUDD_ECOLI
BacMap:90111376
General Reference:
  • Aoyama, K., Haase, A. M., Reeves, P. R. (1994). "Evidence for effect of random genetic drift on G+C content after lateral transfer of fucose pathway genes to Escherichia coli K-12." Mol Biol Evol 11:829-838. Pubmed: 7815923
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Frick, D. N., Townsend, B. D., Bessman, M. J. (1995). "A novel GDP-mannose mannosyl hydrolase shares homology with the MutT family of enzymes." J Biol Chem 270:24086-24091. Pubmed: 7592609
  • Gabelli, S. B., Azurmendi, H. F., Bianchet, M. A., Amzel, L. M., Mildvan, A. S. (2006). "X-ray, NMR, and mutational studies of the catalytic cycle of the GDP-mannose mannosyl hydrolase reaction." Biochemistry 45:11290-11303. Pubmed: 16981689
  • Gabelli, S. B., Bianchet, M. A., Azurmendi, H. F., Xia, Z., Sarawat, V., Mildvan, A. S., Amzel, L. M. (2004). "Structure and mechanism of GDP-mannose glycosyl hydrolase, a Nudix enzyme that cleaves at carbon instead of phosphorus." Structure 12:927-935. Pubmed: 15274914
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Legler, P. M., Massiah, M. A., Bessman, M. J., Mildvan, A. S. (2000). "GDP-mannose mannosyl hydrolase catalyzes nucleophilic substitution at carbon, unlike all other Nudix hydrolases." Biochemistry 39:8603-8608. Pubmed: 10913267
  • Legler, P. M., Massiah, M. A., Mildvan, A. S. (2002). "Mutational, kinetic, and NMR studies of the mechanism of E. coli GDP-mannose mannosyl hydrolase, an unusual Nudix enzyme." Biochemistry 41:10834-10848. Pubmed: 12196023
  • Stevenson, G., Andrianopoulos, K., Hobbs, M., Reeves, P. R. (1996). "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid." J Bacteriol 178:4885-4893. Pubmed: 8759852
  • Xia, Z., Azurmendi, H. F., Lairson, L. L., Withers, S. G., Gabelli, S. B., Bianchet, M. A., Amzel, L. M., Mildvan, A. S. (2005). "Mutational, structural, and kinetic evidence for a dissociative mechanism in the GDP-mannose mannosyl hydrolase reaction." Biochemistry 44:8989-8997. Pubmed: 15966723