Calcium/proton antiporter (P31801)

Identification
Name:Calcium/proton antiporter
Synonyms:Not Available
Gene Name:chaA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in cation transmembrane transporter activity
Specific Function:According to PubMed:8496184 the natural activity of this protein is as a calcium/proton antiporter, but it can act as a weak sodium/proton antiporter. The presence of a preferred substrate and normal pH cytoplasmic values might prevent significant sodium/proton antiporter activity
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21195CalciumMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04139PotassiumMetaboCard
ECMDB02908SodiumMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
cation transmembrane transporter activity
ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
transmembrane transport
transport
Gene Properties
Blattner:b1216
Gene OrientationCounterclockwise
Centisome Percentage:27.37
Left Sequence End1269972
Right Sequence End1271072
Gene Sequence:
>1101 bp
ATGTCAAATGCTCAAGAGGCGGTAAAAACCCGCCACAAGGAGACTTCGCTTATTTTCCCG
GTTCTGGCGCTGGTAGTGCTGTTCCTGTGGGGAAGCAGCCAGACACTACCAGTGGTCATT
GCCATCAATCTTCTTGCGCTTATTGGTATTTTAAGTAGCGCCTTTAGTGTTGTCCGTCAT
GCGGACGTATTAGCCCATCGCCTGGGAGAACCTTACGGTTCGCTTATTCTTAGCCTTTCA
GTGGTTATTCTTGAAGTCAGTTTGATTTCAGCTTTAATGGCAACCGGCGACGCCGCGCCA
ACGCTAATGCGTGATACGCTCTATTCAATCATTATGATTGTTACCGGTGGGCTGGTTGGC
TTTTCATTATTGTTGGGCGGTCGTAAGTTTGCCACCCAATATATGAATCTGTTTGGTATC
AAGCAGTATTTAATTGCGCTGTTCCCCCTGGCGATAATCGTACTGGTATTTCCAATGGCT
CTGCCTGCGGCGAATTTTTCAACCGGTCAGGCGTTACTGGTAGCATTAATTTCTGCGGCA
ATGTATGGCGTATTTTTGCTGATCCAGACCAAAACGCATCAAAGTTTGTTTGTCTACGAG
CACGAAGATGACAGTGATGATGACGACCCGCATCACGGTAAACCGTCTGCCCATAGCAGC
CTGTGGCATGCTATCTGGTTGATTATCCATCTGATTGCCGTTATTGCGGTGACCAAAATG
AACGCCAGCTCGCTGGAGACATTGCTCGACAGCATGAATGCCCCTGTCGCCTTTACTGGC
TTCCTGGTGGCACTGTTGATTCTGTCGCCGGAAGGTTTAGGTGCATTAAAAGCAGTGTTG
AACAACCAGGTTCAGCGCGCGATGAATCTGTTCTTTGGTTCAGTGTTAGCAACCATTTCG
CTTACCGTACCTGTCGTCACGCTAATTGCCTTTATGACGGGTAACGAATTGCAGTTTGCA
CTTGGTGCGCCAGAAATGGTGGTGATGGTGGCCTCTTTAGTGCTGTGCCATATCTCCTTC
TCCACCGGACGTACTAACGTGCTCAATGGCGCAGCGCATCTGGCACTGTTTGCCGCCTAT
TTGATGACGATATTTGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:366
Protein Molecular Weight:39168
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 17-37
  • 38-58
  • 75-95
  • 107-127
  • 144-164
  • 168-188
  • 217-237
  • 256-276
  • 291-311
  • 319-339
  • 346-366
Protein Sequence:
>Calcium/proton antiporter
MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINLLALIGILSSAFSVVRH
ADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTLMRDTLYSIIMIVTGGLVG
FSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMALPAANFSTGQALLVALISAA
MYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAHSSLWHAIWLIIHLIAVIAVTKM
NASSLETLLDSMNAPVAFTGFLVALLILSPEGLGALKAVLNNQVQRAMNLFFGSVLATIS
LTVPVVTLIAFMTGNELQFALGAPEMVVMVASLVLCHISFSTGRTNVLNGAAHLALFAAY
LMTIFA
References
External Links:
ResourceLink
Uniprot ID:P31801
Uniprot Name:CHAA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651604
Ecogene ID:EG11753
Ecocyc:EG11753
ColiBase:b1216
Kegg Gene:b1216
EchoBASE ID:EB1703
CCDB:CHAA_ECOLI
BacMap:16129179
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ivey, D. M., Guffanti, A. A., Zemsky, J., Pinner, E., Karpel, R., Padan, E., Schuldiner, S., Krulwich, T. A. (1993). "Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene." J Biol Chem 268:11296-11303. Pubmed: 8496184
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232