Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (P31677)

Identification
Name:Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
Synonyms:
  • Osmoregulatory trehalose synthesis protein A
  • Trehalose-6-phosphate synthase
  • UDP-glucose-glucosephosphate glucosyltransferase
Gene Name:otsA
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor. Essential for viability of the cells at low temperatures and at elevated osmotic strength
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glucose 6-phosphate + 1.0UDP-Glucose → 1.0Hydrogen ion + 1.0Trehalose 6-phosphate + 1.0Uridine 5'-diphosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0UDP-Glucose + 1.0Glucose 6-phosphate ↔ 1.0Uridine 5'-diphosphate + 1.0Trehalose 6-phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Uridine 5'-diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0UDP-Glucose + 1.0Alpha-D-glucose 6-phosphate → 1.0Uridine 5'-diphosphate + 1.0Trehalose 6-phosphate + 1.0Hydrogen ion + 1.0Uridine 5'-diphosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0UDP-Glucose + 1.0Mannose 6-phosphate → 1.0alpha,alpha-Trehalose 6-phosphate + 1.0Uridine 5'-diphosphate + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0α-D-glucose 6-phosphate → 1.0Thumb+1.0Thumb+1.0Thumb
1.0UDP-Glucose + 1.0α-D-glucose 6-phosphate → 1.0Hydrogen ion + 1.0Uridine 5'-diphosphate + 1.0Trehalose 6-phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0UDP-Glucose + 1.0Glucose 6-phosphate → 1.0Uridine 5'-diphosphate + 1.0Trehalose 6-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04010alpha,alpha-Trehalose 6-phosphateMetaboCard
ECMDB23064Alpha-D-glucose 6-phosphateMetaboCard
ECMDB01401Glucose 6-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01078Mannose 6-phosphateMetaboCard
ECMDB01124Trehalose 6-phosphateMetaboCard
ECMDB04171UDP-GlucoseMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
GO Classification:
Function
alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
UDP-glucosyltransferase activity
UDP-glycosyltransferase activity
Process
carbohydrate metabolic process
disaccharide metabolic process
metabolic process
oligosaccharide metabolic process
primary metabolic process
trehalose biosynthetic process
trehalose metabolic process
Gene Properties
Blattner:b1896
Gene OrientationCounterclockwise
Centisome Percentage:42.64
Left Sequence End1978212
Right Sequence End1979636
Gene Sequence:
>1425 bp
ATGAGTACTGAAATCAAAACTCAGGTCGTGGTACTTGGGGCAGGCCCCGCAGGTTACTCC
GCTGCCTTCCGTTGCGCTGATTTAGGTCTGGAAACCGTAATCGTAGAACGTTACAACACC
CTTGGCGGTGTTTGCCTGAACGTCGGCTGTATCCCTTCTAAAGCACTGCTGCACGTAGCA
AAAGTTATCGAAGAAGCCAAAGCGCTGGCTGAACACGGTATCGTCTTCGGCGAACCGAAA
ACCGATATCGACAAGATTCGTACCTGGAAAGAGAAAGTGATCAATCAGCTGACCGGTGGT
CTGGCTGGTATGGCGAAAGGCCGCAAAGTCAAAGTGGTCAACGGTCTGGGTAAATTCACC
GGGGCTAACACCCTGGAAGTTGAAGGTGAGAACGGCAAAACCGTGATCAACTTCGACAAC
GCGATCATTGCAGCGGGTTCTCGCCCGATCCAACTGCCGTTTATTCCGCATGAAGATCCG
CGTATCTGGGACTCCACTGACGCGCTGGAACTGAAAGAAGTACCAGAACGCCTGCTGGTA
ATGGGTGGCGGTATCATCGGTCTGGAAATGGGCACCGTTTACCACGCGCTGGGTTCACAG
ATTGACGTGGTTGAAATGTTCGACCAGGTTATCCCGGCAGCTGACAAAGACATCGTTAAA
GTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCC
GTTGAAGCGAAAGAAGACGGCATTTATGTGACGATGGAAGGCAAAAAAGCACCCGCTGAA
CCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTC
GACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCGTTGACAAACAG
CTGCGTACCAACGTACCGCACATCTTTGCTATCGGCGATATCGTCGGTCAACCGATGCTG
GCACACAAAGGTGTTCACGAAGGTCACGTTGCCGCTGAAGTTATCGCCGGTAAGAAACAC
TACTTCGATCCGAAAGTTATCCCGTCCATCGCCTATACCGAACCAGAAGTTGCATGGGTG
GGTCTGACTGAGAAAGAAGCGAAAGAGAAAGGCATCAGCTATGAAACCGCCACCTTCCCG
TGGGCTGCTTCTGGTCGTGCTATCGCTTCCGACTGCGCAGACGGTATGACCAAGCTGATT
TTCGACAAAGAATCTCACCGTGTGATCGGTGGTGCGATTGTCGGTACTAACGGCGGCGAG
CTGCTGGGTGAAATCGGCCTGGCAATCGAAATGGGTTGTGATGCTGAAGACATCGCACTG
ACCATCCACGCGCACCCGACTCTGCACGAGTCTGTGGGCCTGGCGGCAGAAGTGTTCGAA
GGTAGCATTACCGACCTGCCGAACCCGAAAGCGAAGAAGAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:474
Protein Molecular Weight:53611
Protein Theoretical pI:7
PDB File:1UQU
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQPLKKVKKGN
ITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPAWDGYLRVNALLADKLLPL
LQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDY
DLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGP
LPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTS
RGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPL
RDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDRALTMS
LAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKVATFPKLA
References
External Links:
ResourceLink
Uniprot ID:P31677
Uniprot Name:OTSA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674337
PDB ID:1UQU
Ecogene ID:EG11751
Ecocyc:EG11751
ColiBase:b1896
Kegg Gene:b1896
EchoBASE ID:EB1701
CCDB:OTSA_ECOLI
BacMap:16129848
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Giaever, H. M., Styrvold, O. B., Kaasen, I., Strom, A. R. (1988). "Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli." J Bacteriol 170:2841-2849. Pubmed: 3131312
  • Gibson, R. P., Tarling, C. A., Roberts, S., Withers, S. G., Davies, G. J. (2004). "The donor subsite of trehalose-6-phosphate synthase: binary complexes with UDP-glucose and UDP-2-deoxy-2-fluoro-glucose at 2 A resolution." J Biol Chem 279:1950-1955. Pubmed: 14570926
  • Gibson, R. P., Turkenburg, J. P., Charnock, S. J., Lloyd, R., Davies, G. J. (2002). "Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA." Chem Biol 9:1337-1346. Pubmed: 12498887
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Kaasen, I., McDougall, J., Strom, A. R. (1994). "Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex." Gene 145:9-15. Pubmed: 8045430
  • Kandror, O., DeLeon, A., Goldberg, A. L. (2002). "Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures." Proc Natl Acad Sci U S A 99:9727-9732. Pubmed: 12105274