Putrescine-binding periplasmic protein (P31133)

Identification
Name:Putrescine-binding periplasmic protein
Synonyms:Not Available
Gene Name:potF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Putrescine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Putrescine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01414PutrescineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b0854
Gene OrientationClockwise
Centisome Percentage:19.25
Left Sequence End893007
Right Sequence End894119
Gene Sequence:
>1113 bp
ATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAA
CTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCT
GCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTACGTGAAGTC
ACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGCTCTCCTGAAGCGG
CTGCGGAATGCCGCCGACTGGTTTGGCATACTTGCCGCACAAACACTTGCTGTACGCGCC
GCAGTTACGGGTTGTACAAGCGGAAAGAGATTGCGTCTTGTCGATGGAACAGCAATCAGT
GCGCCCGGGGGCGGCAGCGCTGAATGGCGACTACATATGGGATATGATCCTCATACCTGT
CAGTTCACTGATTTTGAGCTAACCGACAGCAGAGACGCTGAACGGCTGGACCGATTTGCG
CAAACGGCAGACGAGATACGCATTGCTGACCGGGGATTCGGTTCGCGTCCCGAATGTATC
CGCTCACTTGCTTTTGGAGAAGCTGATTATATCGTCCGGGTTCACTGGCGAGGATTGCGC
TGGTTAACTGCAGAAGGAATGCGCTTTGACATGATGGGTTTTCTGCGCGGGCTGGATTGC
GGTAAGAACGGTGAAACCACTGTAATGATAGGCAATTCAGGTAATAAAAAAGCCGGAGCT
CCCTTTCCGGCACGTCTCATTGCCGTATCACTTCCTCCCGAAAAAGCATTAATCAGTAAA
ACCCGACTGCTCAGCGAGAATCGTCGAAAAGGACGAGTAGTTCAGGCGGAAACGCTGGAA
GCAGCGGGCCATGTGCTATTGCTAACATCATTACCGGAAGATGAATATTCAGCAGAGCAA
GTGGCTGATTGTTACCGTCTGCGATGGCAAATTGAACTGGCTTTTAAGCGGCTCAAAAGT
TTGCTGCACCTGGATGCTTTGCGTGCAAAGGAACCTGAACTCGCGAAAGCGTGGATATTT
GCTAATCTACTCGCCGCATTTTTAATTGACGACATAATCCAGCCATCGCTGGATTTCCCC
CCCAGAAGTGCCGGATCCGAAAAGAAGAACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:370
Protein Molecular Weight:40839
Protein Theoretical pI:6
PDB File:1A99
Signaling Regions:
  • 1-26
Transmembrane Regions:
  • None
Protein Sequence:
>Putrescine-binding periplasmic protein
MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPDTVANFEKETGIKVVYD
VFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLDKSKLPEWKNLDPELLKLV
AKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSWDLILKPENLEKLKSCGVSFL
DAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLRPNIRYFHSSQYINDLANGDICV
AIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAFFDVFAMPADAKNKDEAYQFLNYLL
RPDVVAHISDHVFYANANKAATPLVSAEVRENPGIYPPADVRAKLFTLKVQDPKIDRVRT
RAWTKVKSGK
References
External Links:
ResourceLink
Uniprot ID:P31133
Uniprot Name:POTF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674283
PDB ID:1A99
Ecogene ID:EG11629
Ecocyc:EG11629
ColiBase:b0854
Kegg Gene:b0854
EchoBASE ID:EB1586
CCDB:POTF_ECOLI
BacMap:16128822
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pistocchi, R., Kashiwagi, K., Miyamoto, S., Nukui, E., Sadakata, Y., Kobayashi, H., Igarashi, K. (1993). "Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome." J Biol Chem 268:146-152. Pubmed: 8416922
  • Vassylyev, D. G., Kashiwagi, T., Tomitori, H., Kashiwagi, K., Igarashi, K., Morikawa, K. (1998). "Crystallization and preliminary X-ray analysis of the periplasmic receptor (PotF) of the putrescine transport system in Escherichia coli." Acta Crystallogr D Biol Crystallogr 54:132-134. Pubmed: 9761835
  • Vassylyev, D. G., Tomitori, H., Kashiwagi, K., Morikawa, K., Igarashi, K. (1998). "Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity." J Biol Chem 273:17604-17609. Pubmed: 9651355