ECMDB: Thiazole synthase (P30139)

Identification

Name:

Thiazole synthase

Synonyms:

Not Available

Gene Name:

thiG

Enzyme Class:

2.8.1.10

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

Cellular Location:

Cytoplasm

SMPDB Pathways:

Thiazole Biosynthesis I PW002041

KEGG Pathways:

Metabolic pathways eco01100
Thiamine metabolism ec00730

KEGG Reactions:

1.0C15815

1.0

1.0Iminoglycine

↔

1.0

1.0C15815 + 1.0L-Tyrosine + 1.0Iminoglycine ↔ 1.04-Methyl-5-(2-phosphoethyl)-thiazole
ReactionCard

1.0

↔

1.0

2.0

1.01-Deoxy-D-xylulose 5-phosphate + 1.02-iminoacetate ↔ 1.02-[(2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2.0Water
ReactionCard

SMPDB Reactions:

1.0

1.0a thiocarboxy-[ThiS-Protein]

→

1.0

1.0a ThiS sulfur-carrier protein

2.0

1.01-Deoxy-D-xylulose 5-phosphate + 1.0Dehydroglycine + 1.0a thiocarboxy-[ThiS-Protein] → 1.02-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + 1.0a ThiS sulfur-carrier protein + 2.0Water
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0S-Adenosylmethionine + 1.0NADPH + 1.0L-Tyrosine → 1.0p-Cresol + 1.05'-Deoxyadenosine + 1.0Dehydroglycine + 1.0Hydrogen ion + 1.0L-Methionine + 1.0NADP
ReactionCard

1.0

1.0thiocarboxy-adenylate-[sulfur-carrier protein ThiS]

→

1.0

1.0[sulfur-carrier protein ThiS]

2.0

1.01-Deoxy-D-xylulose 5-phosphate + 1.02-iminoacetate + 1.0thiocarboxy-adenylate-[sulfur-carrier protein ThiS] → 1.02-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + 1.0[sulfur-carrier protein ThiS] + 2.0Water
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01213	1-Deoxy-D-xylulose 5-phosphate	MetaboCard
ECMDB23053	2-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate	MetaboCard
ECMDB23155	2-iminoacetate	MetaboCard
ECMDB23825	2-[(2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate	MetaboCard
ECMDB20094	4-Methyl-5-(2-phosphoethyl)-thiazole	MetaboCard
ECMDB21174	5'-Deoxyadenosine	MetaboCard
ECMDB21328	Dehydroglycine	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00696	L-Methionine	MetaboCard
ECMDB00158	L-Tyrosine	MetaboCard
ECMDB00217	NADP	MetaboCard
ECMDB04111	NADPH	MetaboCard
ECMDB21349	p-Cresol	MetaboCard
ECMDB01185	S-Adenosylmethionine	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process

Gene Properties

Blattner:

b3991

Gene Orientation

Counterclockwise

Centisome Percentage:

90.31

Left Sequence End

4189888

Right Sequence End

4190658

Gene Sequence:

>771 bp
ATGAAGATTATTACTTGCTATAAGTGCGTGCCTGATGAACAGGATATTGCGGTCAATAAT
GCTGATGGTTCATTAGACTTCAGCAAAGCCGATGCCAAAATAAGCCAATACGATCTCAAC
GCTATTGAAGCGGCTTGCCAGCTAAAGCAACAGGCAGCAGAGGCGCAGGTGACAGCCTTA
AGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGC
CCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACG
GCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGGCGAT
GGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATT
CCGGCAGTTAACGGCGTCAGCAAAATTATCTCCCTGACGGCAGATACCCTCACCGTTGAG
CGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTT
TCCACTGATATCAACTCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAA
AAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTAACGCAGAGGCAGCCTGGTCA
GAACAACAGGTTGCCGCGCCGAAACAGCGCGAACGTCAGCGCATCGTGATTGAAGGCGAC
GGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAA

Protein Properties

Pfam Domain Function:

ThiG (PF05690 )

Protein Residues:

256

Protein Molecular Weight:

26896

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Thiazole synthase
MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIA
AGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLV
QQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVV
DAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSY
FAHATSPLTGFLEASA

References

External Links:

Resource	Link
Uniprot ID:	P30139
Uniprot Name:	THIG_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674296
Ecogene ID:	EG11589
Ecocyc:	EG11589
ColiBase:	b3991
Kegg Gene:	b3991
EchoBASE ID:	EB1547
CCDB:	THIG_ECOLI
BacMap:	33347812

General Reference:

Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Kelleher, N. L., Taylor, S. V., Grannis, D., Kinsland, C., Chiu, H. J., Begley, T. P., McLafferty, F. W. (1998). "Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry." Protein Sci 7:1796-1801. Pubmed: 10082377
Leonardi, R., Fairhurst, S. A., Kriek, M., Lowe, D. J., Roach, P. L. (2003). "Thiamine biosynthesis in Escherichia coli: isolation and initial characterisation of the ThiGH complex." FEBS Lett 539:95-99. Pubmed: 12650933
Vander Horn, P. B., Backstrom, A. D., Stewart, V., Begley, T. P. (1993). "Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12." J Bacteriol 175:982-992. Pubmed: 8432721