Thiazole synthase (P30139)

Identification
Name:Thiazole synthase
Synonyms:Not Available
Gene Name:thiG
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0C15815+1.0Thumb+1.0Iminoglycine ↔ 1.0Thumb
1.0C15815 + 1.0L-Tyrosine + 1.0Iminoglycine ↔ 1.04-Methyl-5-(2-phosphoethyl)-thiazole
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+2.0Thumb
1.01-Deoxy-D-xylulose 5-phosphate + 1.02-iminoacetate ↔ 1.02-[(2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 2.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0a thiocarboxy-[ThiS-Protein] → 1.0Thumb+1.0a ThiS sulfur-carrier protein+2.0Thumb
1.01-Deoxy-D-xylulose 5-phosphate + 1.0Dehydroglycine + 1.0a thiocarboxy-[ThiS-Protein] → 1.02-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + 1.0a ThiS sulfur-carrier protein + 2.0Water
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0S-Adenosylmethionine + 1.0NADPH + 1.0L-Tyrosine → 1.0p-Cresol + 1.05'-Deoxyadenosine + 1.0Dehydroglycine + 1.0Hydrogen ion + 1.0L-Methionine + 1.0NADP
ReactionCard
1.0Thumb+1.0Thumb+1.0thiocarboxy-adenylate-[sulfur-carrier protein ThiS] → 1.0Thumb+1.0[sulfur-carrier protein ThiS]+2.0Thumb
1.01-Deoxy-D-xylulose 5-phosphate + 1.02-iminoacetate + 1.0thiocarboxy-adenylate-[sulfur-carrier protein ThiS] → 1.02-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + 1.0[sulfur-carrier protein ThiS] + 2.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB012131-Deoxy-D-xylulose 5-phosphateMetaboCard
ECMDB230532-((2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphateMetaboCard
ECMDB231552-iminoacetateMetaboCard
ECMDB238252-[(2R,5Z)-2-Carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphateMetaboCard
ECMDB200944-Methyl-5-(2-phosphoethyl)-thiazoleMetaboCard
ECMDB211745'-DeoxyadenosineMetaboCard
ECMDB21328DehydroglycineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00696L-MethionineMetaboCard
ECMDB00158L-TyrosineMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB21349p-CresolMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process
Gene Properties
Blattner:b3991
Gene OrientationCounterclockwise
Centisome Percentage:90.31
Left Sequence End4189888
Right Sequence End4190658
Gene Sequence:
>771 bp
ATGAAGATTATTACTTGCTATAAGTGCGTGCCTGATGAACAGGATATTGCGGTCAATAAT
GCTGATGGTTCATTAGACTTCAGCAAAGCCGATGCCAAAATAAGCCAATACGATCTCAAC
GCTATTGAAGCGGCTTGCCAGCTAAAGCAACAGGCAGCAGAGGCGCAGGTGACAGCCTTA
AGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGC
CCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACG
GCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGGCGAT
GGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATT
CCGGCAGTTAACGGCGTCAGCAAAATTATCTCCCTGACGGCAGATACCCTCACCGTTGAG
CGCGAACTGGAAGATGAAACCGAAACCTTAAGCATTCCGCTGCCTGCGGTTGTTGCTGTT
TCCACTGATATCAACTCCCCACAAATTCCTTCGATGAAAGCCATTCTCGGCGCGGCGAAA
AAGCCCGTCCAGGTATGGTCGGCGGCGGATATTGGTTTTAACGCAGAGGCAGCCTGGTCA
GAACAACAGGTTGCCGCGCCGAAACAGCGCGAACGTCAGCGCATCGTGATTGAAGGCGAC
GGCGAAGAACAGATCGCCGCATTTGCTGAAAATCTTCGCAAAGTCATTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:256
Protein Molecular Weight:26896
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Thiazole synthase
MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIA
AGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLV
QQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVV
DAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSY
FAHATSPLTGFLEASA
References
External Links:
ResourceLink
Uniprot ID:P30139
Uniprot Name:THIG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674296
Ecogene ID:EG11589
Ecocyc:EG11589
ColiBase:b3991
Kegg Gene:b3991
EchoBASE ID:EB1547
CCDB:THIG_ECOLI
BacMap:33347812
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kelleher, N. L., Taylor, S. V., Grannis, D., Kinsland, C., Chiu, H. J., Begley, T. P., McLafferty, F. W. (1998). "Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry." Protein Sci 7:1796-1801. Pubmed: 10082377
  • Leonardi, R., Fairhurst, S. A., Kriek, M., Lowe, D. J., Roach, P. L. (2003). "Thiamine biosynthesis in Escherichia coli: isolation and initial characterisation of the ThiGH complex." FEBS Lett 539:95-99. Pubmed: 12650933
  • Vander Horn, P. B., Backstrom, A. D., Stewart, V., Begley, T. P. (1993). "Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12." J Bacteriol 175:982-992. Pubmed: 8432721