Lipopolysaccharide biosynthesis protein rffC (P27832)

Identification
Name:Lipopolysaccharide biosynthesis protein rffC
Synonyms:Not Available
Gene Name:rffC
Enzyme Class:
Biological Properties
General Function:Involved in protein binding
Specific Function:Transaminase converting thymidine diphosphate(TDP)-4- keto-6-deoxy-D-glucose to TDP-4-amino-4,6-dideoxy-D-galactose, the immediate precursor of the ECA sugar TDP-Fuc4NAc
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary Metabolites: enterobacterial common antigen biosynthesis PW000959
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 2 PW002045
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 3 PW002046
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0dTDP-D-Fucosamine ↔ 1.0Coenzyme A + 1.0dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0 dTDP-thomosamine → 1.0TDP-Fuc4NAc + 1.0Coenzyme A + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0dTDP-D-Fucosamine → 1.0Coenzyme A + 1.0dTDP-4-Acetamido-4,6-dideoxy-D-galactose + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24258 dTDP-thomosamineMetaboCard
ECMDB01206Acetyl-CoAMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB20613dTDP-4-Acetamido-4,6-dideoxy-D-galactoseMetaboCard
ECMDB20612dTDP-D-FucosamineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB23181TDP-Fuc4NAcMetaboCard
GO Classification:
Function
acetyltransferase activity
acyltransferase activity
catalytic activity
N-acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
metabolic process
Gene Properties
Blattner:b3790
Gene OrientationClockwise
Centisome Percentage:85.62
Left Sequence End3972490
Right Sequence End3973164
Gene Sequence:
>675 bp
ATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATGAATACCTTAGCCACA
GGGATGGTTCCTGAAACATCAGTATTGCTGGTTGATGAAAAGCGTGGTGAAGCTAGTATT
AATATTAAGAATACAGATGATCATCCTTCATTGCTTTACACCACCATTGTTGATTTGCCT
GAAAGCAATAAATCAATTCGTTTAATCCCGACTCAGCCCGTTATTCGTGTTGAAGCCGGT
CAAGTACAACAGGTTCGATTTTTACTTCAGGCGACTGTTCCTCTGCAATCAGAAGAGCTG
AAACGCGTAACGTTCGAAGGTATTCCACCAAAAGATGACAAGAGCAGCAGAGTTACTGTT
TCAATTCGTCAGGATCTACCTGTACTGATCCACCCGGCTTCCCTGCCGGAAGAGCGAGAG
ACCTGGAAATTCCTGGAATGGCGTAAAAATGGCGACCAAATTGAGATTAGCAACCCCAGT
AATTATGTCGTTCGTATGACCTTACAATTTAAAACGCTCCCTTCAGGAAAAACGGGGGCT
ATAAATAAGACCTATTTTCTACCACACACCAGTACAACTACTGCATTAACAAACGCTACG
GATACAAAAGTCGAGTTTTACCCAGCCAGCCGATATGGTTATCGCGGTAATAAATATGTC
ACTGACTTAAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:224
Protein Molecular Weight:24220
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide biosynthesis protein rffC
MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRVQAKIAASNTGELDALQ
QLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASAAFAQSRFRAPWYAPDASS
RFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNATDARIGLLAGRGAGAELMQT
ALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVESTAYWLYR
References
External Links:
ResourceLink
Uniprot ID:P27832
Uniprot Name:RFFC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676009
Ecogene ID:EG11455
Ecocyc:EG11455
ColiBase:b3790
Kegg Gene:b3790
EchoBASE ID:EB1424
CCDB:RFFC_ECOLI
BacMap:145698332
General Reference:
  • Barr, K., Rick, P. D. (1993). "Physical map location of the rffC and rffA genes of Escherichia coli." J Bacteriol 175:5738-5739. Pubmed: 8366065
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293