Phosphate acyltransferase (P27247)

Identification
Name:Phosphate acyltransferase
Synonyms:
  • Acyl-ACP phosphotransacylase
  • Acyl-[acyl-carrier-protein]--phosphate acyltransferase
  • Phosphate-acyl-ACP acyltransferase
Gene Name:plsX
Enzyme Class:Not Available
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA
Cellular Location:Cytoplasm.
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Acyl-CoA+1.0Acyl-CoA ↔ 1.01-Acyl-sn-glycerol 3-phosphate+1.0Thumb+1.01-Acyl-sn-glycerol 3-phosphate
1.0Glycerol 3-phosphate + 1.0Acyl-CoA + 1.0Acyl-CoA ↔ 1.01-Acyl-sn-glycerol 3-phosphate + 1.0Coenzyme A + 1.01-Acyl-sn-glycerol 3-phosphate
ReactionCard
1.0Acyl-[acyl-carrier protein]+1.0Thumb ↔ 1.01-Acyl-sn-glycerol 3-phosphate+1.0Acyl-carrier protein
1.0Acyl-[acyl-carrier protein] + 1.0Glycerol 3-phosphate ↔ 1.01-Acyl-sn-glycerol 3-phosphate + 1.0Acyl-carrier protein
ReactionCard
Complex Reactions:
1.0Dodecanoyl-ACP (n-C12:0ACP)+1.0Thumb+1.0Thumb → 1.0acyl carrier protein+1.0Thumb
1.0Dodecanoyl-ACP (n-C12:0ACP) + 1.0Hydrogen ion + 1.0Phosphate → 1.0acyl carrier protein + 1.0Dodecanoly-phosphate (n-C12:0)
ReactionCard
1.0Thumb+1.0cis-octadec-11-enoyl-[acyl-carrier protein] (n-C18:1)+1.0Thumb → 1.0acyl carrier protein+1.0Thumb
1.0Hydrogen ion + 1.0cis-octadec-11-enoyl-[acyl-carrier protein] (n-C18:1) + 1.0Phosphate → 1.0acyl carrier protein + 1.0Octadecanoyl-phosphate (n-C18:1)
ReactionCard
1.0Thumb+1.0Myristoyl-ACP (n-C14:0ACP)+1.0Thumb → 1.0acyl carrier protein+1.0Thumb
1.0Hydrogen ion + 1.0Myristoyl-ACP (n-C14:0ACP) + 1.0Phosphate → 1.0acyl carrier protein + 1.0Tetradecanoyl-phosphate (n-C14:0)
ReactionCard
1.0Thumb+1.0Octadecanoyl-ACP (n-C18:0ACP)+1.0Thumb → 1.0acyl carrier protein+1.0Thumb
1.0Hydrogen ion + 1.0Octadecanoyl-ACP (n-C18:0ACP) + 1.0Phosphate → 1.0acyl carrier protein + 1.0Octadecanoyl-phosphate (n-C18:0)
ReactionCard
1.0Thumb+1.0Palmitoyl-ACP (n-C16:0ACP)+1.0Thumb → 1.0acyl carrier protein+1.0Thumb
1.0Hydrogen ion + 1.0Palmitoyl-ACP (n-C16:0ACP) + 1.0Phosphate → 1.0acyl carrier protein + 1.0Hexadecanoyl-phosphate (n-C16:0)
ReactionCard
1.0Acyl-[acyl-carrier-protein]+1.0Thumb → 1.0acyl-phosphate+1.0[acyl-carrier-protein]
1.0Acyl-[acyl-carrier-protein] + 1.0Inorganic phosphate → 1.0acyl-phosphate + 1.0[acyl-carrier-protein]
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01423Coenzyme AMetaboCard
ECMDB21210Dodecanoly-phosphate (n-C12:0)MetaboCard
ECMDB00126Glycerol 3-phosphateMetaboCard
ECMDB21226Hexadecanoyl-phosphate (n-C16:0)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB21258Octadecanoyl-phosphate (n-C18:0)MetaboCard
ECMDB21259Octadecanoyl-phosphate (n-C18:1)MetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB21286Tetradecanoyl-phosphate (n-C14:0)MetaboCard
GO Classification:
Function
catalytic activity
Process
carboxylic acid metabolic process
cellular metabolic process
fatty acid biosynthetic process
fatty acid metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
Gene Properties
Blattner:b1090
Gene OrientationClockwise
Centisome Percentage:24.72
Left Sequence End1146844
Right Sequence End1147914
Gene Sequence:
>1071 bp
ATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATG
CCTGAACGCAGTAGCTGTCGTTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCAC
GGTACTTTCACCGATTTACTTGATAAGCTCAACCCCGGCGATCTTCTGGTTTTTAATAAT
ACCCGCGTGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTG
CTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCG
CCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACC
GCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATGAACGCTCGGTGCTGGATATT
CTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGACCGTCCGGACGAAGACGCT
GACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCGGTTGCAGCCCCGACC
GCAGGTCTGCATTTTGACGAGCCTTTGCTGGAAAAATTGCGCGCCAAAGGCGTGGAGATG
GCGTTTGTGACGTTGCACGTTGGTGCGGGCACCTTCCAGCCGGTGCGCGTCGACACCATT
GAAGATCACATCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTA
CTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCG
CTGGAAAGCGCGGCTCAGGCAGCGAAAAACGATCTCATTGAACCGTTCTTCGACGATACC
CAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCGATGCGCTGGTGACGAACTTC
CACTTGCCAGAGTCGACGCTGATTATGCTGGTTTCGGCCTTTGCCGGTTATCAACACACC
ATGAACGCCTATAAAGCAGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCG
ATGTTTATCACGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:356
Protein Molecular Weight:38214
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphate acyltransferase
MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNSDAITPLLAKADFEQRSRL
QIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQACVSAGNTGALMGLAKLLLK
PLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQFAIMGSVLAEEVVEIPNPRV
ALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEANELLTGKTDVLVCDGFTGNVTLK
TMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKSLTRRFSHLNPDQYNGACLLGLRGT
VIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAARLESVYPAGFELLDGGKSGTLR
References
External Links:
ResourceLink
Uniprot ID:P27247
Uniprot Name:PLSX_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674545
Ecogene ID:EG11437
Ecocyc:EG11437
ColiBase:b1090
Kegg Gene:b1090
EchoBASE ID:EB1407
CCDB:PLSX_ECOLI
BacMap:90111212
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oh, W., Larson, T. J. (1992). "Physical locations of genes in the rne (ams)-rpmF-plsX-fab region of the Escherichia coli K-12 chromosome." J Bacteriol 174:7873-7874. Pubmed: 1447160
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Yoshimura, M., Oshima, T., Ogasawara, N. (2007). "Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli." BMC Microbiol 7:69. Pubmed: 17645809