Lipopolysaccharide 1,2-N-acetylglucosaminetransferase (P27242)

Identification
Name:Lipopolysaccharide 1,2-N-acetylglucosaminetransferase
Synonyms:Not Available
Gene Name:waaU
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring glycosyl groups
Specific Function:Adds the terminal N-acetyl-D-glucosamine group on the glucose(II) group of LPS
Cellular Location:Membrane; Peripheral membrane protein
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine + 1.0LPS (1-O-antigen) ↔ 1.0Uridine 5'-diphosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0galactosyl-(glucosyl)3-(heptosyl)3-Kdo2-lipid A-bisphosphate + 1.0ADP-L-glycero-beta-D-manno-heptose → 1.0Hydrogen ion + 1.0Adenosine diphosphate + 1.0Lipid A-core + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0a lipopolysaccharide+1.0Thumb ? 1.0a N-acetyl-D-glucosaminyl-lipopolysaccharide+1.0Thumb
1.0a lipopolysaccharide + 1.0Uridine diphosphate-N-acetylglucosamine ? 1.0a N-acetyl-D-glucosaminyl-lipopolysaccharide + 1.0Uridine 5'-diphosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Galactosyl-glucosyl3-heptosyl3-KDO2-lipid A-bisphosphate + 1.0ADP-L-Glycero-D-manno-heptose → 1.0Hydrogen ion + 1.0Lipid A-core + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0glucosyl-glucosyl-galactosyl-glucosyl-inner core oligosaccharide lipid A → 1.0Thumb+1.0core oligosaccharide lipid A+1.0Thumb
1.0ADP-L-Glycero-D-manno-heptose + 1.0glucosyl-glucosyl-galactosyl-glucosyl-inner core oligosaccharide lipid A → 1.0ADP + 1.0core oligosaccharide lipid A + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0N-acetyl-D-glucosaminyllipopolysaccharide
1.0Uridine diphosphate-N-acetylglucosamine + 1.0LPS (1-O-antigen) → 1.0Uridine 5'-diphosphate + 1.0N-acetyl-D-glucosaminyllipopolysaccharide
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01341ADPMetaboCard
ECMDB24180ADP-L-glycero-beta-D-manno-heptoseMetaboCard
ECMDB20113ADP-L-Glycero-D-manno-heptoseMetaboCard
ECMDB24227galactosyl-(glucosyl)3-(heptosyl)3-Kdo2-lipid A-bisphosphateMetaboCard
ECMDB20534Galactosyl-glucosyl3-heptosyl3-KDO2-lipid A-bisphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20535Lipid A-coreMetaboCard
ECMDB21088LPS (1-O-antigen)MetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
ECMDB00290Uridine diphosphate-N-acetylglucosamineMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
Process
metabolic process
Gene Properties
Blattner:b3623
Gene OrientationCounterclockwise
Centisome Percentage:81.82
Left Sequence End3796262
Right Sequence End3797335
Gene Sequence:
>1074 bp
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCC
AGACAATTCATTGAAAATAAAAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCA
AGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTAACTTGGCAACTGTGGACATT
TTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTA
AATATGGTACTGCAAGGATTTGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCA
GATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACGCTCATACCGTAGTGTGGAAA
ATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCC
CTGCTTGATTTACAGGGTTTGGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAAT
ATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTGTAATGGCCGATGCTTACCCG
CATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTC
TTAAAAAGTAAGATGAGTGGGTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACT
TACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGCAATTTGCAGCTACTCTTAGA
CCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTG
ATGGGTAAAGCCACTAGAGCGTGGGGAATAATCAAGGACTTATATATTGTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:357
Protein Molecular Weight:41729
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide 1,2-N-acetylglucosaminetransferase
MRLGTFHKKKRFYINKIKINFLSFLFRNKINNQITDPAQVKSCLIIHDNNKLGDLIVLSS
IYRELYSKGVKITLLTNRKGGEFLSNNKNIFEFCIKESTGFLEMLTLCKHLRDLQFDIVL
DPFETMPSFKHSLILSSLKDSYILGFDHWYKRYYSFYHPHDECLKEHMSTRAIEILKHIY
GEGKFSTNYDLHLPVDVEDKIKEFIGDTRIVIINPLGAKKICRLTFEQIKVIYQEVKTHF
ENYRIIFTGLPQDLLTIPILEIETLPFDEFIYTVALTKYSDFVISVDTALVHIAAAYHKP
TLAFYPNSRTPEYPSHLIWSPNHHKSIQIVSPTYTVKDIDTETLTNSVKRLSCIDKK
References
External Links:
ResourceLink
Uniprot ID:P27242
Uniprot Name:WAAU_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675493
Ecogene ID:EG11423
Ecocyc:EG11423
ColiBase:b3623
Kegg Gene:b3623
EchoBASE ID:EB1393
CCDB:WAAU_ECOLI
BacMap:16131494
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Klena, J. D., Pradel, E., Schnaitman, C. A. (1992). "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." J Bacteriol 174:4746-4752. Pubmed: 1624462
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620