Holo-[acyl-carrier-protein] synthase (P24224)

Identification
Name:Holo-[acyl-carrier-protein] synthase
Synonyms:
  • Holo-ACP synthase
  • 4'-phosphopantetheinyl transferase AcpS
Gene Name:acpS
Enzyme Class:
Biological Properties
General Function:Involved in magnesium ion binding
Specific Function:Transfers the 4'-phosphopantetheine moiety from coenzyme A to the 'Ser-36' of acyl-carrier-protein
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
  • Pantothenate and CoA biosynthesis ec00770
KEGG Reactions:
1.0Thumb+1.0Apo-[acyl-carrier-protein] ↔ 1.0Thumb+1.0Acyl-carrier protein+1.0Acyl-carrier protein
1.0Coenzyme A + 1.0Apo-[acyl-carrier-protein] ↔ 1.0Adenosine 3',5'-diphosphate + 1.0Acyl-carrier protein + 1.0Acyl-carrier protein
ReactionCard
Complex Reactions:
1.0apoprotein [acyl carrier protein]+1.0Thumb → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0apoprotein [acyl carrier protein] + 1.0Coenzyme A → 1.0acyl carrier protein + 1.0Hydrogen ion + 1.0Adenosine 3',5'-diphosphate
ReactionCard
1.0CoA-(4'-phosphopantetheine)+1.0apo-[acyl-carrier-protein] → 1.0Thumb+1.0holo-[acyl-carrier-protein]
1.0CoA-(4'-phosphopantetheine) + 1.0apo-[acyl-carrier-protein] → 1.0Adenosine 3',5'-diphosphate + 1.0holo-[acyl-carrier-protein]
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00061Adenosine 3',5'-diphosphateMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
holo-[acyl-carrier-protein] synthase activity
ion binding
magnesium ion binding
metal ion binding
phosphotransferase activity, for other substituted phosphate groups
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
carboxylic acid metabolic process
cellular metabolic process
fatty acid biosynthetic process
fatty acid metabolic process
macromolecule biosynthetic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
Gene Properties
Blattner:b2563
Gene OrientationCounterclockwise
Centisome Percentage:58.16
Left Sequence End2698640
Right Sequence End2699020
Gene Sequence:
>381 bp
ATGGCAATATTAGGTTTAGGCACGGATATTGTGGAGATCGCTCGCATCGAAGCGGTGATC
GCCCGATCCGGTGATCGCCTGGCACGCCGCGTATTAAGCGATAACGAATGGGCTATCTGG
AAAACGCACCACCAGCCGGTGCGTTTTCTGGCGAAGCGTTTTGCTGTGAAAGAAGCCGCA
GCAAAAGCGTTTGGCACCGGGATCCGCAATGGTCTGGCGTTTAATCAATTTGAAGTATTC
AATGATGAGCTCGGCAAACCACGGCTACGGCTATGGGGCGAGGCATTAAAACTGGCGGAA
AAGCTGGGCGTTGCAAATATGCATGTAACGCTGGCAGATGAGCGGCACTATGCTTGTGCC
ACGGTAATTATTGAAAGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:126
Protein Molecular Weight:14052
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Holo-[acyl-carrier-protein] synthase
MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHHQPVRFLAKRFAVKEAA
AKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKLGVANMHVTLADERHYACA
TVIIES
References
External Links:
ResourceLink
Uniprot ID:P24224
Uniprot Name:ACPS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675454
Ecogene ID:EG10247
Ecocyc:EG10247
ColiBase:b2563
Kegg Gene:b2563
EchoBASE ID:EB0243
CCDB:ACPS_ECOLI
BacMap:16130488
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Flugel, R. S., Hwangbo, Y., Lambalot, R. H., Cronan, J. E. Jr, Walsh, C. T. (2000). "Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli." J Biol Chem 275:959-968. Pubmed: 10625633
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lam, H. M., Tancula, E., Dempsey, W. B., Winkler, M. E. (1992). "Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations." J Bacteriol 174:1554-1567. Pubmed: 1537800
  • Lambalot, R. H., Walsh, C. T. (1995). "Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase." J Biol Chem 270:24658-24661. Pubmed: 7559576
  • Takiff, H. E., Baker, T., Copeland, T., Chen, S. M., Court, D. L. (1992). "Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon." J Bacteriol 174:1544-1553. Pubmed: 1537799