Formate dehydrogenase, nitrate-inducible, major subunit (P24183)

Identification
Name:Formate dehydrogenase, nitrate-inducible, major subunit
Synonyms:
  • Anaerobic formate dehydrogenase major subunit
  • Formate dehydrogenase-N subunit alpha
  • FDH-N subunit alpha
Gene Name:fdnG
Enzyme Class:
Biological Properties
General Function:Involved in formate dehydrogenase (NAD+) activity
Specific Function:Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Formic acid + 1.0NAD ↔ 1.0Hydrogen ion + 1.0Carbon dioxide + 1.0NADH
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Formic acid + 1.0Quinone ↔ 1.0Carbon dioxide + 1.0Hydroquinone
ReactionCard
Complex Reactions:
2.0Thumb+1.0Menaquinone 8+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
2.0Hydrogen ion + 1.0Menaquinone 8 + 1.0Formic acid → 1.0Menaquinol 8 + 1.0Carbon dioxide + 1.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
2.0Hydrogen ion + 1.0Ubiquinone-8 + 1.0Formic acid → 1.0Ubiquinol-8 + 1.0Carbon dioxide + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB00142Formic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
cation binding
electron carrier activity
formate dehydrogenase activity
ion binding
metal ion binding
molybdenum ion binding
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
transition metal ion binding
Process
cellular metabolic process
cellular respiration
energy derivation by oxidation of organic compounds
generation of precursor metabolites and energy
metabolic process
oxidation reduction
Gene Properties
Blattner:b1474
Gene OrientationClockwise
Centisome Percentage:33.31
Left Sequence End1545425
Right Sequence End1548472
Gene Sequence:
>3048 bp
ATGGACGTCAGTCGCAGACAATTTTTTAAAATCTGCGCGGGCGGTATGGCTGGAACAACA
GTAGCGGCATTGGGCTTTGCCCCGAAGCAAGCACTGGCTCAGGCGCGAAACTACAAATTA
TTACGCGCTAAAGAGATCCGTAACACCTGCACATACTGTTCCGTAGGTTGCGGGCTATTG
ATGTATAGCCTGGGTGATGGCGCAAAAAACGCCAGAGAAGCGATTTATCACATTGAAGGT
GACCCGGATCATCCGGTAAGCCGTGGTGCGCTGTGCCCGAAAGGGGCCGGTTTGCTGGAT
TACGTCAACAGTGAAAACCGTCTGCGCTACCCGGAATATCGTGCGCCAGGTTCTGACAAA
TGGCAGCGCATTAGCTGGGAAGAAGCATTCTCCCGTATTGCGAAGCTGATGAAAGCTGAC
CGTGACGCTAACTTTATTGAAAAGAACGAGCAGGGCGTAACGGTAAACCGTTGGCTTTCT
ACCGGTATGCTGTGTGCCTCCGGTGCCAGCAACGAAACCGGGATGCTGACCCAGAAATTT
GCCCGCTCCCTCGGGATGCTGGCGGTAGACAACCAGGCGCGCGTCTGACACGGACCAACG
GTAGCAAGTCTTGCTCCAACATTTGGTCGCGGTGCGATGACCAACCACTGGGTGGATATC
AAAAACGCTAACGTCGTGATGGTGATGGGCGGTAACGCTGCTGAAGCGCATCCCGTCGGT
TTCCGCTGGGCGATGGAAGCGAAAAACAACAACGACGCAACCTTGATCGTTGTCGATCCC
CGTTTTACGCGTACCGCTTCTGTGGCGGATATTTACGCGCCTATTCGTTCCGGTACGGAC
ATTACGTTCCTGTCTGGCGTTTTGCGCTACCTGATCGAAAACAACAAAATCAACGCCGAA
TACGTTAAGCATTACACCAACGCCAGCCTGCTGGTGCGTGATGATTTTGCTTTCGAAGAC
GGTCTGTTCAGCGGCTACGACGCTGAAAAACGTCAATACGATAAATCGTCCTGGAACTAT
CAGCTCGATGAAAACGGCTATGCGAAACGCGATGAAACACTGACTCATCCGCGCTGTGTG
TGGAACCTGCTGAAAGAGCACGTTTCCCGCTACACGCCGGACGTCGTTGAAAACATCTGC
GGTACGCCAAAAGCCGACTTCCTGAAAGTGTGTGAAGTGCTGGCCTCCACCAGCGCACCG
GATCGCACAACCACCTTCCTGTACGCGCTGGGCTGGACGCAGCACACTGTGGGTGCGCAG
AACATCCGTACTATGGCGATGATCCAGTTGCTGCTCGGTAACATGGGTATGGCCGGTGGC
GGCGTGAACGCATTGCGTGGTCACTCCAACATTCAGGGCTTGACTGACTTAGGCCTGCTC
TCTACCAGCCTGCCAGGTTATCTGACGCTGCCGTCAGAAAAACAGGTTGATTTGCAGTCG
TATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAAC
TATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAG
AACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTAT
TTCAACATGATGGATGAAGGCAAAGTCACCGGTTATTTCTGCCAGGGCTTTAACCCGGTT
GCGTCCTTCCCGGACAAAAACAAAGTGGTGAGCTGCCTGAGCAAGCTGAAGTACATGGTG
GTTATCGATCCGCTGGTGACTGAAACCTCTACCTTCTGGCAGAACCACGGTGAGTCGAAC
GATGTCGATCCGGCGTCTATTCAGACTGAAGTATTCCGTCTGCCTTCGACCTGCTTTGCT
GAAGAAGATGGTTCTATCGCTAACTCCGGTCGCTGGTTGCAGTGGCACTGGAAAGGTCAG
GACGCGCCGGGCGAAGCGCGTAACGACGGTGAAATTCTGGCGGGTATCTACCATCATCTG
CGCGAGCTGTACCAGTCCGAAGGTGGTAAAGGCGTAGAACCGCTGATGAAGATGAGCTGG
AACTACAAGCAGCCGCACGAACCGCAATCTGACGAAGTGGCTAAAGAGAACAACGGCTAC
GCGCTGGAAGATCTCTATGACGCTAATGGCGTGCTTATTGCGAAGAAAGGTCAGTTGCTG
AGTAGCTTTGCGCATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACC
GGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGT
CTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAAC
CGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGG
AACGGCAGCAAGTGGACGGGTAACGATATTCCTGACTTCGGCAATGCCGCACCGGGTACG
CCAACCGGGCCGTTTATCATGCAGCCGGAAGGGATGGGACGCCTGTTTGCCATCAACAAA
ATGGCGGAAGGTCCGTTCCCGGAACACTACGAGCCGATTGAAACGCCGCTGGGCACTAAC
CCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTG
CGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCAC
TTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTG
GAAATCAGCGAAACGCTGGCGGCGGCGAAAGGCATTAATAATGGCGATCGTGTCACTGTC
TCCAGCAAGCGTGGCTTTATCCGCGCGGTGGCTGTGGTAACGCGTCGTCTGAAACCGCTG
AATGTAAATGGTCAGCAGGTTGAAACGGTGGGTATTCCAATCCACTGGGGCTTTGAGGGT
GTCGCGCGTAAAGGTTATATCGCTAACACTCTGACGCCGAATGTCGGTGATGCAAACTCG
CAAACGCCGGAATATAAAGCGTTCTTAGTCAACATCGAGAAGGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:1015
Protein Molecular Weight:112963
Protein Theoretical pI:7
PDB File:1KQG
Signaling Regions:
  • 1-33
Transmembrane Regions:
  • None
Protein Sequence:
>Formate dehydrogenase, nitrate-inducible, major subunit
MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAKEIRNTCTYCSVGCGLL
MYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYVNSENRLRYPEYRAPGSDK
WQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLSTGMLCASGASNETGMLTQKF
ARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWVDIKNANVVMVMGGNAAEAHPVG
FRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIRSGTDITFLSGVLRYLIENNKINAE
YVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYDKSSWNYQLDENGYAKRDETLTHPRCV
WNLLKEHVSRYTPDVVENICGTPKADFLKVCEVLASTSAPDRTTTFLYALGWTQHTVGAQ
NIRTMAMIQLLLGNMGMAGGGVNALRGHSNIQGLTDLGLLSTSLPGYLTLPSEKQVDLQS
YLEANTPKATLADQVNYWSNYPKFFVSLMKSFYGDAAQKENNWGYDWLPKWDQTYDVIKY
FNMMDEGKVTGYFCQGFNPVASFPDKNKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESN
DVDPASIQTEVFRLPSTCFAEEDGSIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHL
RELYQSEGGKGVEPLMKMSWNYKQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLL
SSFAHLRDDGTTASSCWIYTGSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYN
RASADINGKPWDPKRMLIQWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINK
MAEGPFPEHYEPIETPLGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEH
FHTWTKHALLNAIAQPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPL
NVNGQQVETVGIPIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA
References
External Links:
ResourceLink
Uniprot ID:P24183
Uniprot Name:FDNG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674982
PDB ID:1KQG
Ecogene ID:EG11227
Ecocyc:EG11227
ColiBase:b1474
Kegg Gene:b1474
EchoBASE ID:EB1209
CCDB:FDNG_ECOLI
BacMap:16129433
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Berg, B. L., Li, J., Heider, J., Stewart, V. (1991). "Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine." J Biol Chem 266:22380-22385. Pubmed: 1834669
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jormakka, M., Tornroth, S., Byrne, B., Iwata, S. (2002). "Molecular basis of proton motive force generation: structure of formate dehydrogenase-N." Science 295:1863-1868. Pubmed: 11884747
  • Tullman-Ercek, D., DeLisa, M. P., Kawarasaki, Y., Iranpour, P., Ribnicky, B., Palmer, T., Georgiou, G. (2007). "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." J Biol Chem 282:8309-8316. Pubmed: 17218314