Mannose-1-phosphate guanylyltransferase (P24174)

Identification
Name:Mannose-1-phosphate guanylyltransferase
Synonyms:
  • GDP-mannose pyrophosphorylase
  • GMP
  • GMPP
Gene Name:manC
Enzyme Class:
Biological Properties
General Function:Involved in nucleotidyltransferase activity
Specific Function:Involved in the biosynthesis of the capsular polysaccharide colanic acid
Cellular Location:Not Available
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • Mannose Metabolism PW000822
  • colanic acid building blocks biosynthesis PW000951
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Guanosine triphosphate + 1.0D-Mannose 1-phosphate ↔ 1.0Pyrophosphate + 1.0Guanosine diphosphate mannose
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0D-Mannose 1-phosphate + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0Pyrophosphate + 1.0Guanosine diphosphate mannose
ReactionCard
1.0α-D-mannose 1-phosphate+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Pyrophosphate
1.0α-D-mannose 1-phosphate + 1.0Guanosine triphosphate + 1.0Hydrogen ion → 1.0Guanosine diphosphate mannose + 1.0Pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Guanosine triphosphate + 1.0D-Mannose 1-phosphate ↔ 1.0Pyrophosphate + 1.0Guanosine diphosphate mannose
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0D-Mannose 1-phosphate + 1.0Guanosine triphosphate → 1.0Guanosine diphosphate mannose + 1.0Pyrophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanosine diphosphate + 1.0Hydrogen ion + 1.0D-Mannose 1-phosphate → 1.0Guanosine diphosphate mannose + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01436D-Mannose 1-phosphateMetaboCard
ECMDB01201Guanosine diphosphateMetaboCard
ECMDB01163Guanosine diphosphate mannoseMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
catalytic activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
carbohydrate metabolic process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b2049
Gene OrientationCounterclockwise
Centisome Percentage:45.72
Left Sequence End2121108
Right Sequence End2122544
Gene Sequence:
>1437 bp
ATGGCGCAGTCGAAACTCTATCCAGTTGTGATGGCAGGTGGCTCCGGTAGCCGCTTATGG
CCGCTTTCCCGCGTACTTTATCCCAAGCAGTTTTTATGCCTGAAAGGCGATCTCACCATG
CTGCAAACCACCATCTGCCGCCTGAACGGCGTGGAGTGCGAAAGCCCGGTGGTGATTTGC
AATGAGCAGCACCGCTTTATTGTCGCGGAACAGCTGCGTCAACTGAACAAACTTACCGAG
AACATTATTCTCGAACCGGCAGGGCGAAACACGGCACCTGCCATTGCGCTGGCGGCGCTG
GCGGCAAAACGTCATAGCCCGGAGAGCGACCCGTTAATGCTGGTATTGGCGGCGGATCAT
GTGATTGCCGATGAAGACGCGTTCCGTGCCGCCGTGCGTAATGCCATGCCATATGCCGAA
GCGGGCAAGCTGGTGACCTTCGGCATTGTGCCGGATCTACCAGAAACCGGTTATGGCTAT
ATTCGTCGCGGTGAAGTGTCTGCGGGTGAGCAGGATATGGTGGCCTTTGAAGTGGCGCAG
TTTGTCGAAAAACCGAATCTGGAAACCGCTCAGGCCTATGTGGCAAGCGGCGAATATTAC
TGGAACAGCGGTATGTTCCTGTTCCGCGCCGGACGCTATCTCGAAGAACTGAAAAAATAT
CGCCCGGATATCCTCGATGCCTGTGAAAAAGCGATGAGCGCCGTCGATCCGGATCTCAAT
TTTATTCGCGTGGATGAAGAAGCGTTTCTCGCCTGCCCGGAAGAGTCGGTGGATTACGCG
GTCATGGAACGTACGGCAGATGCTGTTGTGGTGCCGATGGATGCGGGCTGGAGCGATGTT
GGCTCCTGGTCTTCATTATGGGAGATCAGCGCCCACACCGCCGAGGGCAACGTTTGCCAC
GGCGATGTGATTAATCACAAAACTGAAAACAGCTATGTGTATGCTGAATCTGGCCTGGTC
ACCACCGTCGGGGTGAAAGATCTGGTAGTGGTGCAGACCAAAGATGCGGTGCTGATTGCC
GACCGTAACGCGGTACAGGATGTGAAAAAAGTGGTCGAGCAGATCAAAGCCGATGGTCGC
CATGAGCATCGGGTGCATCGCGAAGTGTATCGTCCGTGGGGCAAATATGACTCTATCGAC
GCGGGCGACCGCTACCAGGTGAAACGCATCACCGTGAAACCGGGCGAGGGCTTGTCGGTA
CAGATGCACCATCACCGCGCGGAACACTGGGTGGTTGTCGCGGGAACGGCAAAAGTCACC
ATTGATGGTGATATCAAACTGCTTGGTGAAAACGAGTCCATTTATATTCCGCTGGGGGCG
ACGCATTGCCTGGAAAACCCGGGGAAAATTCCGCTCGATTTAATTGAAGTGCGCTCCGGC
TCTTATCTCGAAGAGGATGATGTGGTGCGTTTCGCGGATCGCTACGGACGGGTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:478
Protein Molecular Weight:53016
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Mannose-1-phosphate guanylyltransferase
MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVIC
NEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADH
VIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRRGEVSAGEQDMVAFEVAQ
FVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLN
FIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCH
GDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGR
HEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQMHHHRAEHWVVVAGTAKVT
IDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
References
External Links:
ResourceLink
Uniprot ID:P24174
Uniprot Name:MANC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736755
Ecogene ID:EG10161
Ecocyc:EG10161
ColiBase:b2049
Kegg Gene:b2049
EchoBASE ID:EB0159
CCDB:MANC_ECOLI
BacMap:16129989
General Reference:
  • Aoyama, K., Haase, A. M., Reeves, P. R. (1994). "Evidence for effect of random genetic drift on G+C content after lateral transfer of fucose pathway genes to Escherichia coli K-12." Mol Biol Evol 11:829-838. Pubmed: 7815923
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Stevenson, G., Andrianopoulos, K., Hobbs, M., Reeves, P. R. (1996). "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid." J Bacteriol 178:4885-4893. Pubmed: 8759852