Lipopolysaccharide heptosyltransferase 1 (P24173)

Identification
Name:Lipopolysaccharide heptosyltransferase 1
Synonyms:Not Available
Gene Name:rfaC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transferase activity, transferring glycosyl groups
Specific Function:Heptose transfer to the lipopolysaccharide core. It transfers the innnermost heptose to [4'-P](3-deoxy-D-manno- octulosonic acid)2-IVA
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0heptosyl-Kdo2-lipid A+1.0Thumb+1.0Thumb
1.0(KDO)2-lipid A + 1.0ADP-L-glycero-beta-D-manno-heptose → 1.0Hydrogen ion + 1.0Adenosine diphosphate + 1.0heptosyl-Kdo2-lipid A + 1.0ADP + 1.0Heptosyl-KDO2-lipid A
ReactionCard
EcoCyc Reactions:
1.0α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA+1.0Thumb ? 1.0Thumb+1.0heptosyl-Kdo2-lipid IVA+1.0Thumb
1.0α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IVA + 1.0ADP-L-Glycero-D-manno-heptose ? 1.0Hydrogen ion + 1.0heptosyl-Kdo2-lipid IVA + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0KDO2-Lipid A + 1.0ADP-L-Glycero-D-manno-heptose → 1.0Heptosyl-KDO2-lipid A + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0heptosyl-kdo2-lipidA
1.0ADP-L-Glycero-D-manno-heptose + 1.0KDO2-Lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0heptosyl-kdo2-lipidA
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24179(KDO)2-lipid AMetaboCard
ECMDB01341ADPMetaboCard
ECMDB24180ADP-L-glycero-beta-D-manno-heptoseMetaboCard
ECMDB20113ADP-L-Glycero-D-manno-heptoseMetaboCard
ECMDB20526Heptosyl-KDO2-lipid AMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21031KDO2-Lipid AMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
Process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3621
Gene OrientationClockwise
Centisome Percentage:81.77
Left Sequence End3794002
Right Sequence End3794961
Gene Sequence:
>960 bp
ATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGAT
TCTCTGACTGCGGTTAGCCGTCAGGATGAGAAACTGGATATTAACATCGATGAAGAAGTG
CATCGTCTGCGTGAAAAAAGCGTAGAACTGACACGTAAAATCTTCGCCGATCTCGGTGCA
TGGCAGATTGCGCAACTGGCACGCCATCCACAGCGTCCTTATACCCTGGATTACGTTCGC
CTGGCATTTGATGAATTTGACGAACTGGCTGGCGACCGCGCGTATGCAGACGATAAAGCT
ATCGTCGGTGGTATCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAA
GGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTAC
CGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTT
ATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCC
ATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATC
GGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTG
CAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTGCGTCCATTCTGTGGAAGAGC
GCCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAA
CTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAA
GCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTA
AGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:319
Protein Molecular Weight:35544
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide heptosyltransferase 1
MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQIPSWHAAVERVIPVAIR
RWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAALVTRLAHGVKHGMDWQTAR
EPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQGDYAIAQHFLTNLPTDAGEY
AVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWGAPHEEERAKRLAEGFAYVEVLP
KMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNITVYGPTDPGLIGGYGKNQMVCRAP
RENLINLNSQAVLEKLSSL
References
External Links:
ResourceLink
Uniprot ID:P24173
Uniprot Name:RFAC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674373
Ecogene ID:EG11189
Ecocyc:EG11189
ColiBase:b3621
Kegg Gene:b3621
EchoBASE ID:EB1175
CCDB:RFAC_ECOLI
BacMap:16131492
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chen, L., Coleman, W. G. Jr (1993). "Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis." J Bacteriol 175:2534-2540. Pubmed: 8478319
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Klena, J. D., Pradel, E., Schnaitman, C. A. (1992). "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." J Bacteriol 174:4746-4752. Pubmed: 1624462
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620