ECMDB: Lipopolysaccharide heptosyltransferase 1 (P24173)

Identification

Name:

Lipopolysaccharide heptosyltransferase 1

Synonyms:

Not Available

Gene Name:

rfaC

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in transferase activity, transferring glycosyl groups

Specific Function:

Heptose transfer to the lipopolysaccharide core. It transfers the innnermost heptose to [4'-P](3-deoxy-D-manno- octulosonic acid)2-IVA

Cellular Location:

Not Available

SMPDB Pathways:

Lipopolysaccharide biosynthesis PW000831
lipopolysaccharide biosynthesis II PW001905
lipopolysaccharide biosynthesis III PW002059

KEGG Pathways:

Lipopolysaccharide biosynthesis ec00540

SMPDB Reactions:

1.0

→

1.0

1.0Adenosine diphosphate

1.0heptosyl-Kdo2-lipid A

1.0

1.0(KDO)2-lipid A + 1.0ADP-L-glycero-beta-D-manno-heptose → 1.0Hydrogen ion + 1.0Adenosine diphosphate + 1.0heptosyl-Kdo2-lipid A + 1.0ADP + 1.0Heptosyl-KDO2-lipid A
ReactionCard

EcoCyc Reactions:

1.0α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IV_A

1.0

1.0heptosyl-Kdo₂-lipid IV_A

1.0

1.0α-Kdo-(2->4)-α-Kdo-(2->6)-lipid IV_A + 1.0ADP-L-Glycero-D-manno-heptose ? 1.0Hydrogen ion + 1.0heptosyl-Kdo₂-lipid IV_A + 1.0ADP
ReactionCard

1.0

→

1.0

1.0Hydrogen ion + 1.0KDO2-Lipid A + 1.0ADP-L-Glycero-D-manno-heptose → 1.0Heptosyl-KDO2-lipid A + 1.0ADP
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0heptosyl-kdo2-lipidA

1.0ADP-L-Glycero-D-manno-heptose + 1.0KDO2-Lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0heptosyl-kdo2-lipidA
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB24179	(KDO)2-lipid A	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB24180	ADP-L-glycero-beta-D-manno-heptose	MetaboCard
ECMDB20113	ADP-L-Glycero-D-manno-heptose	MetaboCard
ECMDB20526	Heptosyl-KDO2-lipid A	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21031	KDO2-Lipid A	MetaboCard

GO Classification:

Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
Process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
primary metabolic process

Gene Properties

Blattner:

b3621

Gene Orientation

Clockwise

Centisome Percentage:

81.77

Left Sequence End

3794002

Right Sequence End

3794961

Gene Sequence:

>960 bp
ATGAGTCTGAATTTCCTTGATTTTGAACAGCCGATTGCAGAGCTGGAAGCGAAAATCGAT
TCTCTGACTGCGGTTAGCCGTCAGGATGAGAAACTGGATATTAACATCGATGAAGAAGTG
CATCGTCTGCGTGAAAAAAGCGTAGAACTGACACGTAAAATCTTCGCCGATCTCGGTGCA
TGGCAGATTGCGCAACTGGCACGCCATCCACAGCGTCCTTATACCCTGGATTACGTTCGC
CTGGCATTTGATGAATTTGACGAACTGGCTGGCGACCGCGCGTATGCAGACGATAAAGCT
ATCGTCGGTGGTATCGCCCGTCTCGATGGTCGTCCGGTGATGATCATTGGTCATCAAAAA
GGTCGTGAAACCAAAGAAAAAATTCGCCGTAACTTTGGTATGCCAGCGCCAGAAGGTTAC
CGCAAAGCACTGCGTCTGATGCAAATGGCTGAACGCTTTAAGATGCCTATCATCACCTTT
ATCGACACCCCGGGGGCTTATCCTGGCGTGGGCGCAGAAGAGCGTGGTCAGTCTGAAGCC
ATTGCACGCAACCTGCGTGAAATGTCTCGCCTCGGCGTACCGGTAGTTTGTACGGTTATC
GGTGAAGGTGGTTCTGGCGGTGCGCTGGCGATTGGCGTGGGCGATAAAGTGAATATGCTG
CAATACAGCACCTATTCCGTTATCTCGCCGGAAGGTTGTGCGTCCATTCTGTGGAAGAGC
GCCGACAAAGCGCCGCTGGCGGCTGAAGCGATGGGTATCATTGCTCCGCGTCTGAAAGAA
CTGAAACTGATCGACTCCATCATCCCGGAACCACTGGGTGGTGCTCACCGTAACCCGGAA
GCGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTA
AGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAA

Protein Properties

Pfam Domain Function:

Glyco_transf_9 (PF01075 )

Protein Residues:

319

Protein Molecular Weight:

35544

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Lipopolysaccharide heptosyltransferase 1
MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQIPSWHAAVERVIPVAIR
RWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAALVTRLAHGVKHGMDWQTAR
EPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQGDYAIAQHFLTNLPTDAGEY
AVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWGAPHEEERAKRLAEGFAYVEVLP
KMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNITVYGPTDPGLIGGYGKNQMVCRAP
RENLINLNSQAVLEKLSSL

References

External Links:

Resource	Link
Uniprot ID:	P24173
Uniprot Name:	RFAC_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674373
Ecogene ID:	EG11189
Ecocyc:	EG11189
ColiBase:	b3621
Kegg Gene:	b3621
EchoBASE ID:	EB1175
CCDB:	RFAC_ECOLI
BacMap:	16131492

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Chen, L., Coleman, W. G. Jr (1993). "Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis." J Bacteriol 175:2534-2540. Pubmed: 8478319
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Klena, J. D., Pradel, E., Schnaitman, C. A. (1992). "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." J Bacteriol 174:4746-4752. Pubmed: 1624462
Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620