ECMDB: Ferrochelatase (P23871)

Identification

Name:

Ferrochelatase

Synonyms:

Heme synthase
Protoheme ferro-lyase

Gene Name:

hemH

Enzyme Class:

4.99.1.1

Biological Properties

General Function:

Involved in ferrochelatase activity

Specific Function:

Catalyzes the ferrous insertion into protoporphyrin IX

Cellular Location:

Cytoplasm

SMPDB Pathways:

Porphyrin metabolism PW000936

KEGG Pathways:

Metabolic pathways eco01100
Porphyrin and chlorophyll metabolism ec00860

KEGG Reactions:

1.0

1.0Fe2+

↔

1.0

2.0

1.0

1.0Protoporphyrin IX + 1.0Fe2+ ↔ 1.0Heme + 2.0Hydrogen ion + 1.0Fe2+
ReactionCard

SMPDB Reactions:

1.0

→

2.0

1.0

1.0Protoporphyrin IX + 1.0Iron → 2.0Hydrogen ion + 1.0ferroheme b
ReactionCard

EcoCyc Reactions:

1.0

→

2.0

1.0

1.0Iron + 1.0Protoporphyrin IX → 2.0Hydrogen ion + 1.0Heme
ReactionCard

1.0

→

1.0

1.0Iron + 1.0Protoporphyrin IX → 1.0Heme + 1.0Hydrogen ion
ReactionCard

Complex Reactions:

1.0

2.0

→

1.0

1.0Heme + 2.0Hydrogen ion → 1.0Protoporphyrin IX + 1.0Iron
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB23899	Fe2+	MetaboCard
ECMDB24252	ferroheme b	MetaboCard
ECMDB03178	Heme	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00692	Iron	MetaboCard
ECMDB00241	Protoporphyrin IX	MetaboCard

GO Classification:

Function
catalytic activity
ferrochelatase activity
lyase activity
Process
heme biosynthetic process
metabolic process
nitrogen compound metabolic process
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process

Gene Properties

Blattner:

b0475

Gene Orientation

Clockwise

Centisome Percentage:

10.72

Left Sequence End

497279

Right Sequence End

498241

Gene Sequence:

>963 bp
ATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCA
CAGGCGGTGAACGTCACCGTGGCGTATCAAACCTCAGCCGAACCGGCGAAAGTGGCTCAG
GCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGC
GGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCC
AGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCA
AAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCAGCAAACCGGAAGATCTG
ATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCA
CTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCG
ATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTT
AACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGC
GCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTC
GTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGAC
GTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAA
GGTGACGTTCCGGGGCTGGTGAAGGGGAATACCTATCTGACGCCGCAGCAACAAACGGCA
GAACTGACCGGACCGGTGAACAAAGCGATCATCGACACCGCGCAGTTTTTGAAAGAGCAG
GGCAAGGTCCCGGCTGTAGCGAATGATTACAGCCAGTACGTTACCTCGCGCTTCGTGCAA
TAA

Protein Properties

Pfam Domain Function:

Ferrochelatase (PF00762 )

Protein Residues:

320

Protein Molecular Weight:

35884

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Ferrochelatase
MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLLWWPLLRGVILPLRSPR
VAKLYASVWMEGGSPLMVYSRQQQQALAQRLPEMPVALGMSYGSPSLESAVDELLAEHVD
HIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIRDYADNHDYINALANSVRASF
AKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTRELASALGMAPEKVMMTFQSRFGRE
PWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLETLEEIAEQNREVFLGAGGKKYEYIP
ALNATPEHIEMMANLVAAYR

References

External Links:

Resource	Link
Uniprot ID:	P23871
Uniprot Name:	HEMH_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674506
Ecogene ID:	EG10431
Ecocyc:	EG10431
ColiBase:	b0475
Kegg Gene:	b0475
EchoBASE ID:	EB0426
CCDB:	HEMH_ECOLI
BacMap:	16128459

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Frustaci, J. M., O'Brian, M. R. (1993). "The Escherichia coli visA gene encodes ferrochelatase, the final enzyme of the heme biosynthetic pathway." J Bacteriol 175:2154-2156. Pubmed: 8458858
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Miyamoto, K., Kanaya, S., Morikawa, K., Inokuchi, H. (1994). "Overproduction, purification, and characterization of ferrochelatase from Escherichia coli." J Biochem 115:545-551. Pubmed: 8056770
Miyamoto, K., Nakahigashi, K., Nishimura, K., Inokuchi, H. (1991). "Isolation and characterization of visible light-sensitive mutants of Escherichia coli K12." J Mol Biol 219:393-398. Pubmed: 2051480