Ferrochelatase (P23871)

Identification
Name:Ferrochelatase
Synonyms:
  • Heme synthase
  • Protoheme ferro-lyase
Gene Name:hemH
Enzyme Class:
Biological Properties
General Function:Involved in ferrochelatase activity
Specific Function:Catalyzes the ferrous insertion into protoporphyrin IX
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Fe2+ ↔ 1.0Thumb+2.0Thumb+1.0Thumb
1.0Protoporphyrin IX + 1.0Fe2+ ↔ 1.0Heme + 2.0Hydrogen ion + 1.0Fe2+
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb
1.0Protoporphyrin IX + 1.0Iron → 2.0Hydrogen ion + 1.0ferroheme b
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb
1.0Iron + 1.0Protoporphyrin IX → 2.0Hydrogen ion + 1.0Heme
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Iron + 1.0Protoporphyrin IX → 1.0Heme + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+2.0Thumb → 1.0Thumb+1.0Thumb
1.0Heme + 2.0Hydrogen ion → 1.0Protoporphyrin IX + 1.0Iron
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23899Fe2+MetaboCard
ECMDB24252ferroheme bMetaboCard
ECMDB03178HemeMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00692IronMetaboCard
ECMDB00241Protoporphyrin IXMetaboCard
GO Classification:
Function
catalytic activity
ferrochelatase activity
lyase activity
Process
heme biosynthetic process
metabolic process
nitrogen compound metabolic process
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process
Gene Properties
Blattner:b0475
Gene OrientationClockwise
Centisome Percentage:10.72
Left Sequence End497279
Right Sequence End498241
Gene Sequence:
>963 bp
ATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCA
CAGGCGGTGAACGTCACCGTGGCGTATCAAACCTCAGCCGAACCGGCGAAAGTGGCTCAG
GCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGC
GGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCC
AGCCCGTTAGCGGTTGCAGCCAGCCAACAGGTGCCGATTGAAGTCTTCTTGCTGGCGTCA
AAACTGGGTAACTCCGAAGCGCTGGTGGTAAAGAAAACTATCAGCAAACCGGAAGATCTG
ATTGGCAAACGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCA
CTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCG
ATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTT
AACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGC
GCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTTGCCGAGAAACATCCTGAGGTC
GTGAAAGCGTTCGCTAAAAGCGCCATCGATGCTCAGCAACCGTACATTGCTAACCCAGAC
GTGTGGCTGAAACAGCCGGAAAACATCAGCAAACTGGCGCGTTTAAGCGGCGTGCCTGAA
GGTGACGTTCCGGGGCTGGTGAAGGGGAATACCTATCTGACGCCGCAGCAACAAACGGCA
GAACTGACCGGACCGGTGAACAAAGCGATCATCGACACCGCGCAGTTTTTGAAAGAGCAG
GGCAAGGTCCCGGCTGTAGCGAATGATTACAGCCAGTACGTTACCTCGCGCTTCGTGCAA
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:320
Protein Molecular Weight:35884
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ferrochelatase
MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLLWWPLLRGVILPLRSPR
VAKLYASVWMEGGSPLMVYSRQQQQALAQRLPEMPVALGMSYGSPSLESAVDELLAEHVD
HIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIRDYADNHDYINALANSVRASF
AKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTRELASALGMAPEKVMMTFQSRFGRE
PWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLETLEEIAEQNREVFLGAGGKKYEYIP
ALNATPEHIEMMANLVAAYR
References
External Links:
ResourceLink
Uniprot ID:P23871
Uniprot Name:HEMH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674506
Ecogene ID:EG10431
Ecocyc:EG10431
ColiBase:b0475
Kegg Gene:b0475
EchoBASE ID:EB0426
CCDB:HEMH_ECOLI
BacMap:16128459
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Frustaci, J. M., O'Brian, M. R. (1993). "The Escherichia coli visA gene encodes ferrochelatase, the final enzyme of the heme biosynthetic pathway." J Bacteriol 175:2154-2156. Pubmed: 8458858
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Miyamoto, K., Kanaya, S., Morikawa, K., Inokuchi, H. (1994). "Overproduction, purification, and characterization of ferrochelatase from Escherichia coli." J Biochem 115:545-551. Pubmed: 8056770
  • Miyamoto, K., Nakahigashi, K., Nishimura, K., Inokuchi, H. (1991). "Isolation and characterization of visible light-sensitive mutants of Escherichia coli K12." J Mol Biol 219:393-398. Pubmed: 2051480