Identification
Name:Periplasmic dipeptide transport protein
Synonyms:
  • Dipeptide-binding protein
  • DBP
Gene Name:dppA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0Homocarnosine+1.0Thumb+1.0Thumb+1.0Thumb1.0Homocarnosine+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0Homocarnosine + 1.0Adenosine triphosphate + 1.0Water + 1.0Homocarnosine → 1.0Homocarnosine + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00078CysteinylglycineMetaboCard
ECMDB03459D-Alanyl-D-alanineMetaboCard
ECMDB00745HomocarnosineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21237L-ProlinylglycineMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b3544
Gene OrientationCounterclockwise
Centisome Percentage:79.84
Left Sequence End3704121
Right Sequence End3705728
Gene Sequence:
>1608 bp
ATGCGTATTTCCTTGAAAAAGTCAGGGATGCTGAAGCTTGGTCTCAGCCTGGTGGCTATG
ACCGTCGCAGCAAGTGTTCAGGCTAAAACTCTGGTTTATTGCTCAGAAGGATCTCCGGAA
GGGTTTAACCCGCAGCTGTTTACCTCCGGCACCACCTATGACGCCTCTTCCGTCCCGCTT
TATAACCGTCTGGTTGAATTTAAAATCGGCACCACCGAAGTGATCCCGGGCCTCGCTGAA
AAGTGGGAAGTCAGCGAAGACGGTAAAACCTATACCTTCCATCTGCGTAAAGGTGTGAAG
TGGCACGACAATAAAGAATTCAAACCGACGCGTGAACTGAACGCCGATGATGTGGTGTTC
TCGTTCGATCGTCAGAAAAACGCGCAAAACCCGTACCATAAAGTTTCTGGCGGCAGCTAC
GAATACTTCGAAGGCATGGGCTTGCCAGAGCTGATCAGTGAAGTGAAAAAGGTGGACGAC
AACACCGTTCAGTTTGTGCTGACTCGCCCGGAAGCGCCGTTCCTCGCTGACCTGGCAATG
GACTTCGCCTCTATTCTGTCAAAAGAATATGCTGATGCGATGATGAAAGCCGGTACACCG
GAAAAACTGGACCTCAACCCAATCGGAACCGGTCCGTTCCAGTTACAGCAGTATCAAAAA
GATTCCCGTATCCGCTACAAAGCGTTTGATGGCTACTGGGGCACCAAACCGCAGATCGAT
ACGCTGGTTTTCTCTATTACCCCTGACGCTTCCGTGCGTTACGCGAAATTGCAGAAGAAT
GAATGCCAGGTGATGCCGTACCCGAACCCGGCAGATATCGCTCGCATGAAGCAGGATAAA
TCCATCAATCTGATGGAAATGCCGGGGCTGAACGTCGGTTATCTCTCGTATAACGTGCAG
AAAAAACCACTCGATGACGTGAAAGTTCGCCAGGCTCTGACCTACGCGGTGAACAAAGAC
GCGATCATCAAAGCGGTTTATCAGGGCGCGGGCGTATCAGCGAAAAACCTGATCCCGCCA
ACCATGTGGGGCTATAACGACGACGTTCAGGACTACACCTACGATCCTGAAAAAGCGAAA
GCCTTGCTGAAAGAAGCGGGTCTGGAAAAAGGTTTCTCCATCGACCTGTGGGCGATGCCG
GTACAACGTCCGTATAACCCGAACGCTCGCCGCATGGCGGAGATGATTCAGGCAGACTGG
GCGAAAGTCGGCGTGCAGGCCAAAATTGTCACCTACGAATGGGGTGAGTACCTCAAGCGT
GCGAAAGATGGCGAGCACCAGACGGTAATGATGGGCTGGACTGGCGATAACGGGGATCCG
GATAACTTCTTCGCCACCCTGTTCAGCTGCGCCGCCTCTGAACAAGGCTCCAACTACTCA
AAATGGTGCTACAAACCGTTTGAAGATCTGATTCAACCGGCGCGTGCTACCGACGACCAC
AATAAACGCGTTGAACTGTACAAACAAGCGCAGGTGGTGATGCACGATCAGGCTCCGGCA
CTGATCATCGCTCACTCCACCGTGTTTGAACCGGTACGTAAAGAAGTTAAAGGCTATGTG
GTTGATCCATTAGGCAAACATCACTTCGAAAACGTCTCTATCGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:535
Protein Molecular Weight:60293
Protein Theoretical pI:7
PDB File:1DPP
Signaling Regions:
  • 1-28
Transmembrane Regions:
  • None
Protein Sequence:
>Periplasmic dipeptide transport protein
MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNPQLFTSGTTYDASSVPL
YNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWHDNKEFKPTRELNADDVVF
SFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDDNTVQFVLTRPEAPFLADLAM
DFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQYQKDSRIRYKAFDGYWGTKPQID
TLVFSITPDASVRYAKLQKNECQVMPYPNPADIARMKQDKSINLMEMPGLNVGYLSYNVQ
KKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSAKNLIPPTMWGYNDDVQDYTYDPEKAK
ALLKEAGLEKGFSIDLWAMPVQRPYNPNARRMAEMIQADWAKVGVQAKIVTYEWGEYLKR
AKDGEHQTVMMGWTGDNGDPDNFFATLFSCAASEQGSNYSKWCYKPFEDLIQPARATDDH
NKRVELYKQAQVVMHDQAPALIIAHSTVFEPVRKEVKGYVVDPLGKHHFENVSIE
References
External Links:
ResourceLink
Uniprot ID:P23847
Uniprot Name:DPPA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676500
PDB ID:1DPP
Ecogene ID:EG10248
Ecocyc:EG10248
ColiBase:b3544
Kegg Gene:b3544
EchoBASE ID:EB0244
CCDB:DPPA_ECOLI
BacMap:16131416
General Reference:
  • Abouhamad, W. N., Manson, M. D. (1994). "The dipeptide permease of Escherichia coli closely resembles other bacterial transport systems and shows growth-phase-dependent expression." Mol Microbiol 14:1077-1092. Pubmed: 7536291
  • Abouhamad, W. N., Manson, M., Gibson, M. M., Higgins, C. F. (1991). "Peptide transport and chemotaxis in Escherichia coli and Salmonella typhimurium: characterization of the dipeptide permease (Dpp) and the dipeptide-binding protein." Mol Microbiol 5:1035-1047. Pubmed: 1956284
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Dunten, P., Mowbray, S. L. (1995). "Crystal structure of the dipeptide binding protein from Escherichia coli involved in active transport and chemotaxis." Protein Sci 4:2327-2334. Pubmed: 8563629
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Nickitenko, A. V., Trakhanov, S., Quiocho, F. A. (1995). "2 A resolution structure of DppA, a periplasmic dipeptide transport/chemosensory receptor." Biochemistry 34:16585-16595. Pubmed: 8527431
  • Olson, E. R., Dunyak, D. S., Jurss, L. M., Poorman, R. A. (1991). "Identification and characterization of dppA, an Escherichia coli gene encoding a periplasmic dipeptide transport protein." J Bacteriol 173:234-244. Pubmed: 1702779
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841