Sulfate adenylyltransferase subunit 2 (P21156)

Identification
Name:Sulfate adenylyltransferase subunit 2
Synonyms:
  • ATP-sulfurylase small subunit
  • Sulfate adenylate transferase
  • SAT
Gene Name:cysD
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:ATP + sulfate = diphosphate + adenylyl sulfate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Sulfate ↔ 1.0Pyrophosphate + 1.0Adenosine phosphosulfate
ReactionCard
1.0Thumb+1.0Selenate ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Selenate ↔ 1.0Pyrophosphate + 1.0Adenylylselenate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Sulfate+1.0Thumb → 1.0Thumb+1.0Pyrophosphate
1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Sulfate + 1.0Sulfate → 1.0Adenosine phosphosulfate + 1.0Pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Sulfate ↔ 1.0Pyrophosphate + 1.0Adenosine phosphosulfate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Guanosine triphosphate + 1.0Water + 1.0Sulfate → 1.0Adenosine phosphosulfate + 1.0Guanosine diphosphate + 1.0Phosphate + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Sulfate → 1.0Pyrophosphate + 1.0Adenosine phosphosulfate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01003Adenosine phosphosulfateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB21003AdenylylselenateMetaboCard
ECMDB01201Guanosine diphosphateMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB23788SelenateMetaboCard
ECMDB01448SulfateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenylyltransferase activity
catalytic activity
nucleotidyltransferase activity
sulfate adenylyltransferase (ATP) activity
sulfate adenylyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular metabolic process
metabolic process
sulfate reduction
sulfur metabolic process
Gene Properties
Blattner:b2752
Gene OrientationCounterclockwise
Centisome Percentage:61.93
Left Sequence End2873443
Right Sequence End2874351
Gene Sequence:
>909 bp
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCA
GGGGAGCAGTTGTACCGCCATCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATT
GAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGACGGGGCAGCGCGGAACGGTA
GGTATGGGCATTCCTGGCTCAATTTCGCCTTACACCGGTGTGGTGAAGAATGCCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTG
CGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCG
GGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTC
AATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAA
CAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTG
AGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTA
GCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTA
TATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCG
GTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCA
CAAGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:302
Protein Molecular Weight:35188
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Sulfate adenylyltransferase subunit 2
MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVMLHLARKAFYPGTLPFPL
LHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLK
QALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWHNYNGQINKGES
IRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLERDGMLMMIDDNRIDLQPGEVIK
KRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVSTTSERQGRVIDRDQAGSMELKKRQG
YF
References
External Links:
ResourceLink
Uniprot ID:P21156
Uniprot Name:CYSD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674535
Ecogene ID:EG10186
Ecocyc:EG10186
ColiBase:b2752
Kegg Gene:b2752
EchoBASE ID:EB0183
CCDB:CYSD_ECOLI
BacMap:16130659
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Leyh, T. S., Taylor, J. C., Markham, G. D. (1988). "The sulfate activation locus of Escherichia coli K12: cloning, genetic, and enzymatic characterization." J Biol Chem 263:2409-2416. Pubmed: 2828368
  • Leyh, T. S., Vogt, T. F., Suo, Y. (1992). "The DNA sequence of the sulfate activation locus from Escherichia coli K-12." J Biol Chem 267:10405-10410. Pubmed: 1316900
  • Malo, M. S., Loughlin, R. E. (1990). "Promoter elements and regulation of expression of the cysD gene of Escherichia coli K-12." Gene 87:127-131. Pubmed: 2185135