ECMDB: Sulfate adenylyltransferase subunit 2 (P21156)

Identification

Name:

Sulfate adenylyltransferase subunit 2

Synonyms:

ATP-sulfurylase small subunit
Sulfate adenylate transferase
SAT

Gene Name:

cysD

Enzyme Class:

2.7.7.4

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

ATP + sulfate = diphosphate + adenylyl sulfate

Cellular Location:

Not Available

SMPDB Pathways:

Sulfur metabolism PW000922
cysteine biosynthesis PW000800
sulfur metabolism (butanesulfonate) PW000923
sulfur metabolism (ethanesulfonate) PW000925
sulfur metabolism (isethionate) PW000926
sulfur metabolism (methanesulfonate) PW000927
sulfur metabolism (propanesulfonate) PW000924

KEGG Pathways:

Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Monobactam biosynthesis eco00261
Purine metabolism ec00230
Selenoamino acid metabolism ec00450
Sulfur metabolism ec00920

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Sulfate ↔ 1.0Pyrophosphate + 1.0Adenosine phosphosulfate
ReactionCard

1.0

1.0Selenate

↔

1.0

1.0Adenosine triphosphate + 1.0Selenate ↔ 1.0Pyrophosphate + 1.0Adenylylselenate
ReactionCard

SMPDB Reactions:

1.0

1.0Sulfate

1.0

→

1.0

1.0Pyrophosphate

1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Sulfate + 1.0Sulfate → 1.0Adenosine phosphosulfate + 1.0Pyrophosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Sulfate ↔ 1.0Pyrophosphate + 1.0Adenosine phosphosulfate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Guanosine triphosphate + 1.0Water + 1.0Sulfate → 1.0Adenosine phosphosulfate + 1.0Guanosine diphosphate + 1.0Phosphate + 1.0Pyrophosphate
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Sulfate → 1.0Pyrophosphate + 1.0Adenosine phosphosulfate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01003	Adenosine phosphosulfate	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB21003	Adenylylselenate	MetaboCard
ECMDB01201	Guanosine diphosphate	MetaboCard
ECMDB01273	Guanosine triphosphate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard
ECMDB23788	Selenate	MetaboCard
ECMDB01448	Sulfate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
adenylyltransferase activity
catalytic activity
nucleotidyltransferase activity
sulfate adenylyltransferase (ATP) activity
sulfate adenylyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular metabolic process
metabolic process
sulfate reduction
sulfur metabolic process

Gene Properties

Blattner:

b2752

Gene Orientation

Counterclockwise

Centisome Percentage:

61.93

Left Sequence End

2873443

Right Sequence End

2874351

Gene Sequence:

>909 bp
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCA
GGGGAGCAGTTGTACCGCCATCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATT
GAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGACGGGGCAGCGCGGAACGGTA
GGTATGGGCATTCCTGGCTCAATTTCGCCTTACACCGGTGTGGTGAAGAATGCCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTG
CGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCG
GGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTC
AATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAA
CAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTG
AGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTA
GCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTA
TATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCG
GTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCA
CAAGAGTAA

Protein Properties

Pfam Domain Function:

PAPS_reduct (PF01507 )

Protein Residues:

302

Protein Molecular Weight:

35188

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Sulfate adenylyltransferase subunit 2
MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVMLHLARKAFYPGTLPFPL
LHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINPFVHGSAKHTDIMKTEGLK
QALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDPKNQRPELWHNYNGQINKGES
IRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLERDGMLMMIDDNRIDLQPGEVIK
KRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVSTTSERQGRVIDRDQAGSMELKKRQG
YF

References

External Links:

Resource	Link
Uniprot ID:	P21156
Uniprot Name:	CYSD_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674535
Ecogene ID:	EG10186
Ecocyc:	EG10186
ColiBase:	b2752
Kegg Gene:	b2752
EchoBASE ID:	EB0183
CCDB:	CYSD_ECOLI
BacMap:	16130659

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Leyh, T. S., Taylor, J. C., Markham, G. D. (1988). "The sulfate activation locus of Escherichia coli K12: cloning, genetic, and enzymatic characterization." J Biol Chem 263:2409-2416. Pubmed: 2828368
Leyh, T. S., Vogt, T. F., Suo, Y. (1992). "The DNA sequence of the sulfate activation locus from Escherichia coli K-12." J Biol Chem 267:10405-10410. Pubmed: 1316900
Malo, M. S., Loughlin, R. E. (1990). "Promoter elements and regulation of expression of the cysD gene of Escherichia coli K-12." Gene 87:127-131. Pubmed: 2185135