Phosphonates import ATP-binding protein PhnC (P16677)

Identification
Name:Phosphonates import ATP-binding protein PhnC
Synonyms:Not Available
Gene Name:phnC
Enzyme Class:
Biological Properties
General Function:Inorganic ion transport and metabolism
Specific Function:Part of the ABC transporter complex PhnCDE involved in phosphonates, phosphate esters, phosphite and phosphate import. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0DL-O-Phosphoserine → 1.0Hydrogen ion + 1.0Phosphate + 1.0Adenosine diphosphate + 1.0DL-O-Phosphoserine + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.03-Aminopropylphosphonate + 1.0Adenosine triphosphate + 1.0Water → 1.0ADP + 1.0Phosphate + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0phosphonate(Out) → 1.0Thumb+1.0Thumb+1.0phosphonate(In)
1.0Adenosine triphosphate + 1.0Water + 1.0phosphonate(Out) → 1.0ADP + 1.0Inorganic phosphate + 1.0phosphonate(In)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB203293-AminopropylphosphonateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB24073DL-O-PhosphoserineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
anion transmembrane transporter activity
ATP binding
ATPase activity
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
ion transmembrane transporter activity
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
organic anion transmembrane transporter activity
phosphonate transmembrane transporter activity
phosphonate transmembrane-transporting ATPase activity
purine nucleoside binding
pyrophosphatase activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
ion transport
organic anion transport
phosphonate transport
transport
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>789 bp
ATGCAAACGATTATCCGTGTCGAGAAGCTCGCCAAAACCTTCAATCAGCATCAGGCGCTG
CATGCGGTTGATCTGAACATTCATCACGGTGAAATGGTGGCTCTGCTTGGGCCGTCGGGT
TCCGGAAAATCCACCCTTTTACGTCACTTAAGCGGTTTGATTACCGGCGATAAATCTGTC
GGTAGCCATATCGAGCTGCTGGGCCGCACAGTCCAGCGCGAAGGCCGCCTGGCCCGCGAT
ATCCGCAAAAGCCGCGCCCATACCGGCTACATATTCCAACAATTCAACCTGGTGAACCGC
CTGAGCGTACTGGAGAACGTGCTGATTGGCGCGCTCGGCAGCACGCCGTTCTGGCGCACC
TGTTTTAGCTGGTTCACCGGCGAGCAGAAACAGCGCGCGTTACAGGCGCTGACCCGCGTT
GGCATGGTGCATTTTGCCCATCAGCGCGTTTCCACCCTCTCCGGCGGCCAGCAGCAACGT
GTGGCGATTGCCCGTGCGCTGATGCAGCAGGCGAAAGTGATTCTGGCCGATGAACCCATC
GCCTCGCTGGACCCAGAATCAGCGCGCATCGTGATGGACACCCTGCGCGACATCAACCAG
AACGACGGCATCACCGTGGTCGTCACGCTGCATCAGGTGGATTACGCCCTGCGCTACTGC
GAACGCATCGTCGCCCTGCGCCAGGGGCACGTCTTCTACGACGGCAGCAGCCAACAGTTT
GATAACGAACGTTTTGACCATCTCTACCGCAGCATTAACCGCGTCGAAGAGAACGCGAAA
GCTGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:262
Protein Molecular Weight:29430
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphonates import ATP-binding protein PhnC
MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKSTLLRHLSGLITGDKSV
GSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLSVLENVLIGALGSTPFWRT
CFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQRVAIARALMQQAKVILADEPI
ASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALRYCERIVALRQGHVFYDGSSQQF
DNERFDHLYRSINRVEENAKAA
References
External Links:
ResourceLink
Uniprot ID:P16677
Uniprot Name:PHNC_ECOLI
GenBank Gene ID:D90227
Genebank Protein ID:216591
CCDB:PHNC_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Chen, C. M., Ye, Q. Z., Zhu, Z. M., Wanner, B. L., Walsh, C. T. (1990). "Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B." J Biol Chem 265:4461-4471. Pubmed: 2155230
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Makino, K., Kim, S. K., Shinagawa, H., Amemura, M., Nakata, A. (1991). "Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12." J Bacteriol 173:2665-2672. Pubmed: 1840580
  • Metcalf, W. W., Wanner, B. L. (1991). "Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi." J Bacteriol 173:587-600. Pubmed: 1846145
  • Metcalf, W. W., Wanner, B. L. (1993). "Mutational analysis of an Escherichia coli fourteen-gene operon for phosphonate degradation, using TnphoA' elements." J Bacteriol 175:3430-3442. Pubmed: 8388873