Fe(3+) dicitrate-binding periplasmic protein (P15028)

Identification
Name:Fe(3+) dicitrate-binding periplasmic protein
Synonyms:
  • Iron(III) dicitrate-binding periplasmic protein
Gene Name:fecB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in binding
Specific Function:Binds citrate-dependent Fe(3+); part of the binding- protein-dependent transport system for uptake of citrate-dependent Fe(3+)
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Fe(III)dicitrate → 1.0ADP + 2.0Citric acid + 1.0Fe3+ + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00094Citric acidMetaboCard
ECMDB21213Fe(III)dicitrateMetaboCard
ECMDB21395Fe3+MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
cation transmembrane transporter activity
di-, tri-valent inorganic cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
ion transmembrane transporter activity
iron ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
high-affinity iron ion transport
ion transport
iron ion transmembrane transport
iron ion transport
metal ion transport
transition metal ion transport
transport
Gene Properties
Blattner:b4290
Gene OrientationCounterclockwise
Centisome Percentage:97.24
Left Sequence End4511429
Right Sequence End4512331
Gene Sequence:
>903 bp
ATGGATGCACCGAGTACCACACCGCATGACGCGGTATTTAAACAATTTTTAATGCATGCG
GAGACGGCTCGCGACTTTCTGGAGATACATTTGCCAGTGGAATTACGCGAACTTTGTGAC
CTCAACACGCTTCATTTAGAGTCGGGGAGTTTCATTGAAGAGAGCCTGAAAGGACACAGC
ACGGACGTGCTCTATTCCGTGCAAATGCAGGGCAATCCCGGTTATCTGCATGTTGTGATT
GAACACCAAAGCAAGCCGGATAAGAAAATGGCCTTTCGCATGATGCGTTATTCTATAGCC
GCCATGCACCGGCATCTGGAGGCTGACCACGATAAGCTGCCGCTGGTGGTGCCGATACTG
TTTTATCAGGGCGAGGCCACACCTTATCCGCTATCAATGTGCTGGTTTGATATGTTTTAC
TCGCCGGAGCTGGCGCGACGCGTCTATAACAGTCCTTTCCCGCTGGTGGATATCACCATC
ACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAA
CATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAA
GGGTACACTAGCGGAAGTCAGTTAGTTGCCATGCAAAACTATATGCTGCAACGCGGTCAT
ACTGAACAAGCGGATTTGTTTTACGGTGTGTTGAGAGACAGGGAAACGGGAGGGGAGTCT
ATGATGACGCTGGCGCAGTGGTTTGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGA
AGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAA
GACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATT
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:300
Protein Molecular Weight:33146
Protein Theoretical pI:9
Signaling Regions:
  • 1-21
Transmembrane Regions:
  • None
Protein Sequence:
>Fe(3+) dicitrate-binding periplasmic protein
MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLELSFADALAAVDVIPIG
IADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDLIIADSSRHAGVYIALQQI
APVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQHKERMAQWASQLPKGTRVAFG
TSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPSIGLEQLLAVNPAWLLVAHYREE
SIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRGIFAAERIAADTVKIFHHQPLTVVK
References
External Links:
ResourceLink
Uniprot ID:P15028
Uniprot Name:FECB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674347
Ecogene ID:EG10287
Ecocyc:EG10287
ColiBase:b4290
Kegg Gene:b4290
EchoBASE ID:EB0283
CCDB:FECB_ECOLI
BacMap:162135917
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Staudenmaier, H., Van Hove, B., Yaraghi, Z., Braun, V. (1989). "Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli." J Bacteriol 171:2626-2633. Pubmed: 2651410