Probable bifunctional chitinase/lysozyme (P13656)

Identification
Name:Probable bifunctional chitinase/lysozyme
Synonyms:
  • Chitinase
  • Lysozyme
Gene Name:chiA
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity, hydrolyzing O-glycosyl compounds
Specific Function:Bifunctional enzyme with lysozyme/chitinase activity
Cellular Location:Periplasm
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism I PW000886
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • Amino sugar and nucleotide sugar metabolism III PW000895
KEGG Pathways:
  • Amino sugar and nucleotide sugar metabolism ec00520
Transports:
    Transport References:Not Available
    KEGG Reactions:
    1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
    1.0Chitin + 1.0Water ↔ 1.0N-Acetyl-D-glucosamine + 1.0Chitin
    ReactionCard
    1.0Thumb+1.0Thumb ↔ 1.0N-Acetyl-D-glucosaminide+1.0Thumb
    1.0Chitin + 1.0Water ↔ 1.0N-Acetyl-D-glucosaminide + 1.0Chitin
    ReactionCard
    SMPDB Reactions:
    1.0Thumb+1.0Thumb → 1.0Chitobiose+1.0Thumb+1.0Thumb
    1.0Chitin + 1.0Water → 1.0Chitobiose + 1.0Chitin + 1.0Chitobiose
    ReactionCard
    1.0Thumb+1.0Thumb → 1.0N-Acetyl-D-glucosamine+1.0Thumb+1.0Thumb
    1.0Chitin + 1.0Water → 1.0N-Acetyl-D-glucosamine + 1.0Chitin + 1.0N-Acetylglucosamine
    ReactionCard
    Metabolites:
    ECMDB IDNameView
    ECMDB21648ChitinMetaboCard
    ECMDB03556ChitobioseMetaboCard
    ECMDB00215N-Acetyl-D-glucosamineMetaboCard
    ECMDB21561N-AcetylglucosamineMetaboCard
    ECMDB00494WaterMetaboCard
    GO Classification:
    Component
    extracellular region
    Function
    binding
    carbohydrate binding
    catalytic activity
    hydrolase activity
    hydrolase activity, acting on glycosyl bonds
    hydrolase activity, hydrolyzing O-glycosyl compounds
    Process
    carbohydrate metabolic process
    metabolic process
    primary metabolic process
    Gene Properties
    Blattner:b3338
    Gene OrientationCounterclockwise
    Centisome Percentage:74.69
    Left Sequence End3465182
    Right Sequence End3467875
    Gene Sequence:
    >2694 bp
ATGAAATTAAATATATTTACTAAATCTATGATTGGTATGGGGCTGGTGTGTTCCGCTCTG
CCAGCATTGGCAATGGAAGCATGGAATAACCAACAAGGTGGTAATAAATATCAGGTTATT
TTCGATGGCAAAATTTATGAAAATGCCTGGTGGGTTTCTTCTACAAATTGCCCGGGAAAA
GCGAAAGCAAATGATGCAACTAACCCGTGGCGTTTAAAGCGTACCGCAACAGCTGCTGAA
ATTAGTCAGTTTGGCAATACACTTTCCTGCGAAAAGAGCGGCAGCTCATCTTCTTCAAAT
TCAAATACGCCTGCATCCAATACGCCGGCTAATGGCGGTTCGGCTACACCAGCACAGGGC
ACTGTTCCGTCTAATTCTTCTGTAGTTGCCTGGAATAAACAGCAGGGCGGTCAGACCTGG
TATGTCGTCTTTAATGGTGCGGTATATAAAAATGCCTGGTGGGTAGCCTCTTCTAACTGT
CCGGGTGATGCGAAAAGCAATGATGCCAGCAACCCATGGCGTTATGTTCGTGCCGCTACG
GCAACGGAAATCTCAGAAACCAGTAATCCACAGTCCTGTACTTCAGCACCACAGCCTTCA
CCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTCAGCCAGCCCCAGCT
GATAAGTCAAACGACAACTATGCTGTAGTAGCCTGGAAAGGTCAGGAAGGTTCTTCTACA
TGGTACGTTATCTATAACGGCGGCATTTATAAGAACGCCTGGTGGGTAGGCGCGGCAAAT
TGCCCAGGCGATGCGAAAGAAAACGATGCCAGTAACCCATGGCGTTATGTTCGCGCGGCA
ACGGCAACAGAAATCAGCCAGTATGGTAACCCTGGCTCCTGTTCCGTTAAGCCGGATAAT
AATGGCGGTGCTGTGACTCCGGTTGATCCAACTCCGGAAACACCGGTGACCCCAACTCCG
GATAACAGCGAGCCATCAACACCAGCGGATAGCGTTAACGATTACTCATTGCAAGCGTGG
AGCGGCCAGGAAGGTAGCGAAATTTACCATGTTATTTTCAATGGTAATGTTTACAAGAAC
GCCTGGTGGGTTGGGTCTAAAGATTGCCCACGGGGTACCAGCGCTGAAAACTCCAATAAC
CCATGGCGTCTCGAGCGTACAGCTACCGCTGCGGAATTGAGTCAGTACGGTAACCCGACT
ACCTGTGAAATTGATAACGGCGGCGTCATTGTTGCGGATGGTTTCCAGGCCAGCAAAGCG
TACAGCGCGGACAGCATCGTAGATTATAACGATGCACATTATAAAACTTCTGTCGATCAA
GACGCATGGGGCTTTGTCCCGGGCGGCGATAACCCGTGGAAGAAATACGAACCGGCGAAA
GCATGGTCCGCATCCACTGTGTACGTGAAAGGTGATCGCGTTGTTGTTGATGGGCAGGCT
TATGAAGCGCTGTTCTGGACGCAAAGTGACAACCCTGCTCTGGTGGCGAACCAAAACGCC
ACCGGTAGCAATAGCCGCCCGTGGAAGCCGTTAGGTAAGGCTCAGAGCTATAGCAACGAA
GAGCTGAATAATGCGCCGCAGTTTAATCCAGAAACGCTTTATGCCAGCGATACGCTGATT
CGCTTTAACGGTGTGAACTACATTTCTCAGAGTAAAGTGCAGAAAGTTTCTCCTTCTGAC
AGCAACCCGTGGCGTGTTTTTGTTGACTGGACCGGAACCAAAGAGCGCGTAGGTACGCCG
AAGAAAGCGTGGCCGAAACACGTTTATGCACCGTATGTCGACTTTACGCTGAATACGATC
CCGGATCTGGCTGCGCTGGCTAAGAATCATAACGTCAACCACTTCACGCTGGCGTTTGTG
GTGAGTAAAGATGCGAACACCTGTCTGCCGACATGGGGTACCGCTTACGGTATGCAGAAT
TACGCTCAGTACAGCAAAATCAAAGCTCTGCGTGAGGCTGGCGGCGATGTGATGCTGTCT
ATCGGTGGTGCTAACAACGCTCCGCTGGCTGCTTCCTGTAAGAACGTAGACGATCTGATG
CAGCATTATTATGACATCGTTGATAACCTGAACCTCAAAGTCCTGGACTTCGATATCGAA
GGCACCTGGGTTGCGGATCAGGCATCTATTGAACGTCGTAACCTTGCTGTGAAGAAAGTG
CAGGATAAATGGAAGTCAGAAGGCAAAGATATTGCTATCTGGTACACCTTGCCAATTCTG
CCGACTGGCCTGACGCCGGAAGGGATGAATGTCCTGAGCGATGCCAAAGCGAAAGGTGTT
GAGCTGGCGGGTGTGAACGTGATGACAATGGACTACGGTAACGCGATTTGTCAGTCTGCA
AATACCGAAGGCCAGAACATTCACGGTAAGTGTGCAACGTCTGCGATTGCCAACCTGCAT
TCACAATTGAAAGGCCTCCATCCCAATAAGAGCGATGCAGAAATTGACGCTATGATGGGT
ACCACGCCGATGGTTGGCGTGAACGACGTTCAGGGCGAGGTGTTCTATCTCTCTGATGCT
CGTCTGGTCATGCAGGATGCGCAGAAGCGTAATCTCGGTATGGTTGGTATCTGGTCAATC
GCGCGCGACCTGCCGGGCGGCACTAACCTGTCTCCGGAATTCCACGGCCTGACTAAAGAA
CAGGCACCGAAGTACGCATTTAGCGAAATCTTCGCGCCGTTTACTAAGCAATAA
    Protein Properties
    Pfam Domain Function:
    Protein Residues:897
    Protein Molecular Weight:97057
    Protein Theoretical pI:5
    Signaling Regions:
    • 1-24
    Transmembrane Regions:
    • None
    Protein Sequence:
    >Probable bifunctional chitinase/lysozyme
MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGK
AKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQG
TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKSNDASNPWRYVRAAT
ATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQEGSST
WYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQYGNPGSCSVKPDN
NGGAVTPVDPTPETPVTPTPDNSEPSTPADSVNDYSLQAWSGQEGSEIYHVIFNGNVYKN
AWWVGSKDCPRGTSAENSNNPWRLERTATAAELSQYGNPTTCEIDNGGVIVADGFQASKA
YSADSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQA
YEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNEELNNAPQFNPETLYASDTLI
RFNGVNYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTI
PDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLS
IGGANNAPLAASCKNVDDLMQHYYDIVDNLNLKVLDFDIEGTWVADQASIERRNLAVKKV
QDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSA
NTEGQNIHGKCATSAIANLHSQLKGLHPNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDA
RLVMQDAQKRNLGMVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ
    References
    External Links:
    ResourceLink
    Uniprot ID:P13656
    Uniprot Name:CHIA_ECOLI
    GenBank Gene ID:AP009048
    Genebank Protein ID:85676703
    Ecogene ID:EG11237
    Ecocyc:EG11237
    ColiBase:b3338
    Kegg Gene:b3338
    EchoBASE ID:EB1219
    CCDB:CHIA_ECOLI
    BacMap:16131217
    General Reference:
    • Andrews, S. C., Harrison, P. M., Guest, J. R. (1989). "Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12." J Bacteriol 171:3940-3947. Pubmed: 2661540
    • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
    • Francetic, O., Badaut, C., Rimsky, S., Pugsley, A. P. (2000). "The ChiA (YheB) protein of Escherichia coli K-12 is an endochitinase whose gene is negatively controlled by the nucleoid-structuring protein H-NS." Mol Microbiol 35:1506-1517. Pubmed: 10760150
    • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
    • Simpson, H. D., Barras, F. (1999). "Functional analysis of the carbohydrate-binding domains of Erwinia chrysanthemi Cel5 (Endoglucanase Z) and an Escherichia coli putative chitinase." J Bacteriol 181:4611-4616. Pubmed: 10419961