Uridine phosphorylase (P12758)

Identification
Name:Uridine phosphorylase
Synonyms:
  • UPase
  • UrdPase
Gene Name:udp
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring pentosyl groups
Specific Function:Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1- phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0alpha-D-Ribose 1-phosphate+1.0Thumb
1.0Uridine + 1.0Phosphate ↔ 1.0Uracil + 1.0alpha-D-Ribose 1-phosphate + 1.0Ribose-1-phosphate
ReactionCard
1.05-FU+1.0alpha-D-Ribose 1-phosphate ↔ 1.05-Fluorouridine+1.0Thumb
1.05-FU + 1.0alpha-D-Ribose 1-phosphate ↔ 1.05-Fluorouridine + 1.0Phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Uridine + 1.0Phosphate → 1.0Uracil + 1.0Ribose-1-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphate + 1.0Uridine ↔ 1.0Ribose-1-phosphate + 1.0Uracil
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Uridine + 1.0Inorganic phosphate → 1.0Uracil + 1.0Ribose-1-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01351Deoxyribose 1-phosphateMetaboCard
ECMDB00012DeoxyuridineMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB04147Ribose-1-phosphateMetaboCard
ECMDB00300UracilMetaboCard
ECMDB00296UridineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
uridine phosphorylase activity
Process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process
nucleoside phosphate metabolic process
nucleotide catabolic process
nucleotide metabolic process
Gene Properties
Blattner:b3831
Gene OrientationClockwise
Centisome Percentage:86.52
Left Sequence End4014454
Right Sequence End4015215
Gene Sequence:
>762 bp
GTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCAT
GTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCC
TTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAAC
GGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAG
GAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTG
CACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAGTCGCTTTAACTCGCGTTTG
CAAGAACGTATTCGTAAATCTGAAGCGCTAACAGCCGGGAATACCGGTCTGACGCTGAAT
ATTGCGGCGAACTACGGTGGACGTTGGGATATAGTCCAGGGAGTCAGGCAACTGGCTGAA
AAGGTGCAGCAAGGAAACCTGCAACCAGATCAGATAGATGAAGAGATGCTAAACCAGCAT
GTCTGTATGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGAGCATCGC
ATTAGTAACTTTTTGCTTTGGCAAATTGCCTATGCCGAACTTTACTTTACAGATGTTCTC
TGGCCCGATTTCGATGAACAAGACTTTGAAGGGGCGTTAAATGCCTTTGCTAATCGAGAG
CGTCGTTTCGGCGGCACCGAGCCCGGTGATGAAACAGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:253
Protein Molecular Weight:27159
Protein Theoretical pI:6
PDB File:1T0U
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Uridine phosphorylase
MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKLASHREFTTWRAELDGK
PVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHINVGDVLVTTASVRLDGAS
LHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSDTFYPGQERYDTYSGRVVRHF
KGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAGVIVNRTQQEIPNAETMKQTESH
AVKIVVEAARRLL
References
External Links:
ResourceLink
Uniprot ID:P12758
Uniprot Name:UDP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674367
PDB ID:1T0U
Ecogene ID:EG11045
Ecocyc:EG11045
ColiBase:b3831
Kegg Gene:b3831
EchoBASE ID:EB1038
CCDB:UDP_ECOLI
BacMap:16131680
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burling, F. T., Kniewel, R., Buglino, J. A., Chadha, T., Beckwith, A., Lima, C. D. (2003). "Structure of Escherichia coli uridine phosphorylase at 2.0 A." Acta Crystallogr D Biol Crystallogr 59:73-76. Pubmed: 12499542
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Gonzalez-Gil, G., Bringmann, P., Kahmann, R. (1996). "FIS is a regulator of metabolism in Escherichia coli." Mol Microbiol 22:21-29. Pubmed: 8899705
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Henzel, W. J., Billeci, T. M., Stults, J. T., Wong, S. C., Grimley, C., Watanabe, C. (1993). "Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases." Proc Natl Acad Sci U S A 90:5011-5015. Pubmed: 8506346
  • Morgunova, E. Y. u., Mikhailov, A. M., Popov, A. N., Blagova, E. V., Smirnova, E. A., Vainshtein, B. K., Mao, C., Armstrong, S. h. R., Ealick, S. E., Komissarov, A. A., et, a. l. .. (1995). "Atomic structure at 2.5 A resolution of uridine phosphorylase from E. coli as refined in the monoclinic crystal lattice." FEBS Lett 367:183-187. Pubmed: 7796917
  • Veiko, V. P., Chebotaev, D. V., Ovcharova, I. V., Gul'ko, L. B. (1998). "[Protein engineering of uridine phosphorylase from Escherichia coli K-12. I. Cloning and expression of uridine phosphorylase genes from Klebsiella aerogenes and Salmonella typhimurium in E. coli]." Bioorg Khim 24:381-387. Pubmed: 9661793
  • Walton, L., Richards, C. A., Elwell, L. P. (1989). "Nucleotide sequence of the Escherichia coli uridine phosphorylase (udp) gene." Nucleic Acids Res 17:6741. Pubmed: 2674901