6-phospho-beta-glucosidase BglB (P11988)

Identification
Name:6-phospho-beta-glucosidase BglB
Synonyms:Not Available
Gene Name:bglB
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity, hydrolyzing O-glycosyl compounds
Specific Function:Can hydrolyze salicin and arbutin
Cellular Location:Cytoplasmic
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism III PW000895
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Salicyl alcohol+1.0Thumb
1.0Salicin 6-phosphate + 1.0Water ↔ 1.0Salicyl alcohol + 1.0beta-D-Glucose 6-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Cellobiose-6-phosphate + 1.0Water ↔ 1.0D-Glucose + 1.0Glucose 6-phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Salicyl alcohol
1.0Salicin 6-phosphate + 1.0Water → 1.0β-D-glucose 1-phosphate + 1.0Salicyl alcohol
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Arbutin 6-phosphate + 1.0Water → 1.0Glucose 6-phosphate + 1.0Hydroquinone
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Cellobiose-6-phosphate + 1.0Water → 1.0Glucose 6-phosphate + 1.0b-D-Glucose
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0salicyl alcohol
1.0Salicin 6-phosphate + 1.0Water → 1.0Glucose 6-phosphate + 1.0salicyl alcohol
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB215046-Phospho-beta-D-glucosyl-(1,4)-D-glucoseMetaboCard
ECMDB20117Arbutin 6-phosphateMetaboCard
ECMDB00516b-D-GlucoseMetaboCard
ECMDB03498beta-D-Glucose 6-phosphateMetaboCard
ECMDB20267Cellobiose-6-phosphateMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB01401Glucose 6-phosphateMetaboCard
ECMDB02434HydroquinoneMetaboCard
ECMDB20191Salicin 6-phosphateMetaboCard
ECMDB00494WaterMetaboCard
ECMDB24239β-D-glucose 1-phosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
ion binding
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3721
Gene OrientationCounterclockwise
Centisome Percentage:84.06
Left Sequence End3900312
Right Sequence End3901724
Gene Sequence:
>1413 bp
ATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTA
GCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAT
GCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACC
GCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGT
AACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATC
GGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGC
AACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAAC
AAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTG
CCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTT
GGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTT
ATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAA
ATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATC
ATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAG
AAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATG
CTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAAC
GCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATAC
CCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAAC
AGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGT
GGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGC
GGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACC
ACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTT
AAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGC
GGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGC
ACTACTAACACCGCATCTGTTCACGTCCTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:470
Protein Molecular Weight:53161
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>6-phospho-beta-glucosidase BglB
MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKMEPRILGKENIKDVAID
FYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAGLAFYDRLFDEMAQAGIKP
LVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRYQHKVALWLTFNEINMSLHAP
FTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLPEAKIGNMLLGGLVYPLTCQPQD
MLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNITIEMTESDAEDLKHTVDFISFSYY
MTGCVSHDESINKNAQGNILNMIPNPHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIV
ENGLGAKDSVEADGSIQDDYRIAYLNDHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQ
MSKRYGFIYVDRDDNGEGSLTRTRKKSFGWYAEVIKTRGLSLKKITIKAP
References
External Links:
ResourceLink
Uniprot ID:P11988
Uniprot Name:BGLB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675072
Ecogene ID:EG10114
Ecocyc:EG10114
ColiBase:b3721
Kegg Gene:b3721
EchoBASE ID:EB0112
CCDB:BGLB_ECOLI
BacMap:16131589
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bramley, H. F., Kornberg, H. L. (1987). "Nucleotide sequence of bglC, the gene specifying enzymeIIbgl of the PEP:sugar phosphotransferase system in Escherichia coli K12, and overexpression of the gene product." J Gen Microbiol 133:563-573. Pubmed: 3309161
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Schnetz, K., Toloczyki, C., Rak, B. (1987). "Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes." J Bacteriol 169:2579-2590. Pubmed: 3034860