Glycerophosphoryl diester phosphodiesterase_ (P10908)

Identification
Name:Glycerophosphoryl diester phosphodiesterase_
Synonyms:
  • Glycerophosphodiester phosphodiesterase
Gene Name:ugpQ
Enzyme Class:
Biological Properties
General Function:Involved in glycerophosphodiester phosphodiesterase activity
Specific Function:Glycerophosphoryl diester phosphodiesterase hydrolyzes deacylated phospholipids to G3P and the corresponding alcohols
Cellular Location:Cytoplasm
SMPDB Pathways:
  • glycerol metabolism PW000914
  • glycerol metabolism II PW000915
  • glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
  • glycerol metabolism IV (glycerophosphoglycerol) PW000917
  • glycerol metabolism V (glycerophosphoserine) PW000918
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glycerophosphocholine + 1.0Water ↔ 1.0Choline + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glycerylphosphorylethanolamine + 1.0Water ↔ 1.0Ethanolamine + 1.0Glycerol 3-phosphate
ReactionCard
1.0Glycerophosphodiester+1.0Thumb ↔ 1.0Alcohol+1.0Thumb
1.0Glycerophosphodiester + 1.0Water ↔ 1.0Alcohol + 1.0Glycerol 3-phosphate
ReactionCard
SMPDB Reactions:
1.0a glycerophosphodiester+1.0Thumb → 1.0Thumb+1.0Alcohol+1.0Thumb
1.0a glycerophosphodiester + 1.0Water → 1.0Hydrogen ion + 1.0Alcohol + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0sn-glycero-3-phosphocholine + 1.0Water → 1.0Hydrogen ion + 1.0Benzyl alcohol + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0sn-glycero-3-phosphoethanolamine + 1.0Water → 1.0Benzyl alcohol + 1.0Hydrogen ion + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerophosphoglycerol + 1.0Water → 1.0Benzyl alcohol + 1.0Hydrogen ion + 1.0Glycerol 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0 glycerophosphoserine + 1.0Water → 1.0Hydrogen ion + 1.0Benzyl alcohol + 1.0Glycerol 3-phosphate
ReactionCard
EcoCyc Reactions:
1.0a glycerophosphodiester+1.0Thumb → 1.0an alcohol+1.0Thumb+1.0Thumb
1.0a glycerophosphodiester + 1.0Water → 1.0an alcohol + 1.0Glycerol 3-phosphate + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0A glycerophosphodiester+1.0Thumb → 1.0an alcohol+1.0Thumb
1.0A glycerophosphodiester + 1.0Water → 1.0an alcohol + 1.0Glycerol 3-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24250 glycerophosphoserineMetaboCard
ECMDB24002Benzyl alcoholMetaboCard
ECMDB00097CholineMetaboCard
ECMDB00149EthanolamineMetaboCard
ECMDB00131GlycerolMetaboCard
ECMDB00126Glycerol 3-phosphateMetaboCard
ECMDB00086GlycerophosphocholineMetaboCard
ECMDB21223GlycerophosphoglycerolMetaboCard
ECMDB21224GlycerophosphoserineMetaboCard
ECMDB00114GlycerylphosphorylethanolamineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00160InositolMetaboCard
ECMDB00187L-SerineMetaboCard
ECMDB21357Sn-Glycero-3-phospho-1-inositolMetaboCard
ECMDB24248sn-glycero-3-phosphocholineMetaboCard
ECMDB24249sn-glycero-3-phosphoethanolamineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
glycerophosphodiester phosphodiesterase activity
hydrolase activity
hydrolase activity, acting on ester bonds
phosphoric diester hydrolase activity
phosphoric ester hydrolase activity
Process
alcohol metabolic process
alditol metabolic process
glycerol metabolic process
lipid metabolic process
metabolic process
polyol metabolic process
primary metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3449
Gene OrientationCounterclockwise
Centisome Percentage:77.28
Left Sequence End3585393
Right Sequence End3586136
Gene Sequence:
>744 bp
ATGAAAAACACCGAACTGGAACAACTGATTAACGAAAAACTGAACAGCGCGGCGATTAGT
GATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACC
GGTGTCACCGCCAGCCAGGCTTTGCTCGATGAGGCAGTGCGTCTGGGCGCGGATGCGGTC
ATTGTGCATCATGGCTACTTCTGGAAAGGAGAGTCTCCGGTCATTCGCGGAATGAAGCGT
AACCGTTTAAAAACGTTGCTGGCGAATGATATCAACCTGTATGGCTGGCATTTGCCGCTT
GACGCACATCCTGAGCTGGGCAATAACGCACAGCTGGCGGCGTTACTGGGGATCACGGTC
ATGGGCGAAATTGAGCCGTTGGTGCCGTGGGGCGAACTGACCATGCCTGTGCCGGGACTG
GAGCTGGCTTCCTGGATTGAAGCGCGTCTGGGACGTAAGCCGTTATGGTGTGGCGATACC
GGACCTGAGGTGGTCCAGCGCGTCGCCTGGTGCACGGGCGGCGGGCAAAGTTTTATCGAT
AGCGCCGCGCGTTTTGGCGTGGATGCTTTTATTACTGGCGAAGTTTCTGAACAGACCATT
CATTCAGCCCGCGAGCAGGGATTGCATTTTTATGCTGCAGGTCACCATGCCACTGAACGT
GGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTT
ATTGATATTCCTAATCCTGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:247
Protein Molecular Weight:27409
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glycerophosphoryl diester phosphodiesterase
MSNWPYPRIVAHRGGGKLAPENTLASIDVGAKYGHKMIEFDAKLSKDGEIFLLHDDNLER
TSNGWGVAGELNWQDLLRVDAGSWYSKMFKGEPLPLLSQVAERCREHGMMANIEIKPTTG
TGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAAPELPRGLLLDEWRDDWRELT
ARLGCVSIHLNHKLLNKARVMQLKDAGLRILVYTVNKPQRAAELLRWGVDCICTDAIDVI
GPNFTAQ
References
External Links:
ResourceLink
Uniprot ID:P10908
Uniprot Name:UGPQ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062306
Ecogene ID:EG11050
Ecocyc:EG11050
ColiBase:b3449
Kegg Gene:b3449
EchoBASE ID:EB1043
CCDB:UGPQ_ECOLI
BacMap:16131321
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kasahara, M., Makino, K., Amemura, M., Nakata, A. (1989). "Nucleotide sequence of the ugpQ gene encoding glycerophosphoryl diester phosphodiesterase of Escherichia coli K-12." Nucleic Acids Res 17:2854. Pubmed: 2541415
  • Ohshima, N., Yamashita, S., Takahashi, N., Kuroishi, C., Shiro, Y., Takio, K. (2008). "Escherichia coli cytosolic glycerophosphodiester phosphodiesterase (UgpQ) requires Mg2+, Co2+, or Mn2+ for its enzyme activity." J Bacteriol 190:1219-1223. Pubmed: 18083802
  • Overduin, P., Boos, W., Tommassen, J. (1988). "Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system." Mol Microbiol 2:767-775. Pubmed: 3062310
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Tommassen, J., Eiglmeier, K., Cole, S. T., Overduin, P., Larson, T. J., Boos, W. (1991). "Characterization of two genes, glpQ and ugpQ, encoding glycerophosphoryl diester phosphodiesterases of Escherichia coli." Mol Gen Genet 226:321-327. Pubmed: 1851953